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HPLC Frit

HPLC Frit for HPLC Inline Filter

Specification :

Sintered Metal Disc Filter with OEM Pore Size Peek or PCTFE Circle

1.)  316L Stainless Steel for UHPLC, Pore Size: 0.2μ, 0.5μ, 2μ, 5μ, 10μ, 20μ OEM Option 

2.)  Peek or PCTFE Circle Board 

3.) Diameter OEM Size

OD :  6.4 / 6.5 / 14.4 / 19.1 / 22.4 / 38.0mm

 ID : 1.6 / 2.4 / 3.2 / 12.0 / 15.8 / 19.0 / 33.0 mm 

H: 1.6 / 2.0mm

Peek HPLC Frit and Disc

2.1# HPLC Column Cartridge & Cap Frit

Specification
1.) 316L Stainless Steel for UHPLC, Pore Size: 0.2μ, 0.5μ, 2μ
2.) Peek for option for Pore Size: 2μ

2.1 Stainless Steel Cap Frit HPLC Filter

2.1 ultra High Pressure HPLC Column Cartridge

2.1 Peek Cap Frit HPLC Filter

2.1 Peek Normal End-Cap Frit HPLC Column Hardware

3.0# HPLC COLUMN CAP FRITS

Specification :
1.) 316L Stainless Steel for UHPLC, Pore Size: 0.2μ, 0.5μ, 2μ
2. ) Peek for option for Pore Size:  2μ

3.0 Stainless Steel Cap Frit HPLC Filter

3.0 Stainless Steel Frit for HPLC Column

3.0 Peek Cap Frit HPLC Filter

3.0 Peek HPLC Frit for HPLC Column with Small Hole

4.0# HPLC COLUMN Peek CAP FRITS

Specification :
Peek Pore Size:                                            PEEK Big Hole:  0.5μ, 2μ, 5μ, 10μ, 15μ, 20μ 

4.0 Peek Cap Frit Small HPLC Filter

4.0 Peek HPLC Frit with Small HPLC Hole Filter

4.0 Peek Cap Frit Wide HPLC Filter

4.0 Peek Frit with Big Hole Filter for HPLC Colum

4.6# HPLC COLUMN CAP FRITS

Materials
1. 316L Stainless Steel , PEEK

2. Pore Size:                              0.5μ, 2μ, 5μ, 10μ, 15μ, 20μ  

4.6 Peek Cap Frit Big HPLC Filter

4.6 Peek Cap Frit Big HPLC Filter

PEEK Big Hole: 0.5μ, 2μ, 5μ, 10μ, 15μ, 20μ

4.6 Peek Cap Frit Small HPLC Filter

4.6 Peek Cap Frit Small HPLC Filter

4.6 Peek Filter with Stainless Cap Frit for HPLC

4.6 Peek Filter with Stainless Cap Frit for HPLC

20#,30#,50# HPLC COLUMN PEEK CAP FRITS

Specification :
1. PEEK Frit:                                     0.5μ, 2μ, 5μ, 10μ, 15μ, 20μ 
2. PEEK Filter:                            0.5μ, 2μ, 5μ, 10μ, 15μ, 20μ 

10.0 Peek Cap Frit HPLC Filter

#10 Peek Cap Frit HPLC Filter

PEEK Big Hole: 0.5μ, 2μ, 5μ, 10μ, 15μ

20.0 Peek Frit HPLC Filter

#20 Peek Frit HPLC Filter

20.0 Peek Cap Frit HPLC Filter

#20 Peek Cap Frit HPLC Filter

30.0 Peek Frit HPLC Filter

#30 Peek Frit HPLC Filter

30.0 Peek Cap Frit HPLC Filter

#30 Peek Cap Frit HPLC Filter

50mm Peek Frit HPLC Filter

#50mm Peek Frit HPLC Filter

50mm Peek Cap HPLC Frit Filter

#50mm Peek Cap HPLC Frit Filter

DAC HPLC COLUMN Filter

Specification :
1. 316L Stainless Steel for UHPLC, Pore Size:                         0.5μ, 2μ, 5μ, 10μ, 15μ, 20μ, 30μ, 40μ,
2. Pore Size OEM Available

DAC Stainless Steel HPLC Frit Filter

DAC Stainless Steel HPLC Frit Filter

HPLC Frit OEM Supplier

  As a trusted professional OEM supplier of HPLC Column Frit, we, uHPLCs, are committed to providing high-quality HPLC frit and services that exceed our customers’ expectations. Our focus on precision and accuracy for laboratory applications is why we use only the best materials and advanced manufacturing techniques to produce our Ultra High Performance Liquid Chromatography column frits. 

   We understand the challenges scientists and researchers face when seeking to accurately analyze data. That’s why we’ve developed different size frits designed to deliver the highest levels of performance and reliability if Lab Applications. 

   Our column frits are fully tested and proven to provide excellent efficiency, resolution, and peak symmetry, making them ideal for complex sample matrices. We offer a wide range of column frits in various pore sizes and materials to suit different applications. 

UPLC Stainless Stel Frit and Peek HPLC Cartridge
  • Our experts collaborate closely with clients to fully understand their specific needs and provide tailored solutions that align with their individual requirements.

  • We prioritize timely delivery, fast turnaround times, and competitive pricing, establishing us as the preferred HPLC Frit professional OEM supplier for customers globally.

  • uHPLCs is recognized as a dependable and professional OEM supplier of HPLC frits, offering high-quality column frit HPLC designed for outstanding performance and precision in laboratory settings.

  • Partner with us to enhance your research with our superior products and exceptional service, and elevate your research outcomes to the next level.

Improve Your HPLC System by Quality HPLC Column Frit Today

Upgrade your HPLC system with our top-quality Column Frits.

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Precision Masters

The Elite Team Behind HPLC Column Hardware Production

Crafting Excellence

The Pioneers of HPLC Column Hardware Manufacturing

Innovators in Flow

The Leading Professionals of HPLC Column Hardware Design

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High Quality HPLC Column 316L, 316 Stainless Steel Powder Materials

Design New HPLC Columns 100% As Clients Request

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High Quality HPLC Column 316L, 316 Stainless Steel Tube Materials

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HPLC Column FAQ

What is a Frit in HPLC ?

In High-Performance Liquid Chromatography (HPLC), a frit is a porous filter or barrier located at the entrance and/or exit of the chromatographic column. Its main purpose is to prevent particles and debris from entering the column, which could potentially cause clogging and negatively impact the separation process.

The frit is usually made of a porous material, such as stainless steel or PEEK (Polyether Ether Ketone), and acts as a physical barrier while still allowing the mobile phase (solvent and sample) to pass through smoothly. It ensures that only the dissolved analytes in the sample are introduced into the column for separation and analysis, while particulates and larger contaminants are retained outside the column.

The use of a frit is essential to maintain the optimal performance and longevity of the HPLC column, as well as to obtain reliable and accurate analytical results. Regular maintenance and replacement of the frit are necessary to prevent any potential blockages that could affect the chromatographic performance.

Main Features of HPLC Column Frit

As We Know, The HPLC column frit is a crucial component that plays a significant role in the chromatographic process. Here are the main features of an HPLC column frit:

1.Porosity:

The frit is designed to have controlled porosity, allowing the mobile phase (solvent and sample) to pass through while preventing particles and contaminants from entering the column. It acts as a filter, ensuring only the dissolved analytes enter the separation process.

2. Material:

Frits are typically made of materials that are chemically inert and compatible with a wide range of solvents and samples. Stainless steel and PEEK (Polyether Ether Ketone) are commonly used materials due to their stability and resistance to corrosion.

3. Particle Retention:

The frit is designed to effectively retain and prevent particles from passing into the column. This feature ensures the column remains free from blockages, which could otherwise affect the chromatographic performance.

4. Frit Size:

The size of the frit’s pores is carefully chosen based on the specific requirements of the HPLC column and the type of analysis being performed. The frit’s size affects the flow rate and backpressure of the system, making it important to select the appropriate frit dimensions for optimal performance.

5. Compatibility:

The frit material must be compatible with the mobile phase, sample, and the stationary phase of the HPLC column. Compatibility ensures there are no unwanted interactions that could interfere with the separation process.

6. Uniformity:

The frit should be manufactured with high precision to maintain uniformity in porosity and particle retention across the entire surface. A consistent and well-made frit ensures reliable and reproducible chromatographic results.

7. Longevity:

The frit is subject to wear and tear over time due to its continuous use. To ensure the longevity of the column and maintain consistent performance, it is essential to inspect and replace the frit regularly.

8. Easy Replacement:

uHPLCs’ HPLC column frits designed for easy replacement. This allows chromatographers to perform routine maintenance efficiently and minimize downtime in the analysis process.

Overall, the HPLC column frit is a critical component that contributes to the effectiveness and reliability of the chromatographic separation process. Its proper design and maintenance are essential for obtaining accurate and reproducible analytical results in high-performance liquid chromatography.

Types of Frit in HPLC ?

In High-Performance Liquid Chromatography (HPLC), there are primarily two types of frits used at the entrance and/or exit of the chromatographic column:

Stainless Steel Frits:

These frits are made of stainless steel, a durable and chemically resistant material. Stainless steel frits are commonly used in HPLC columns, particularly in analytical and preparative HPLC systems. They come in various porosities and can withstand high pressures and aggressive solvents, making them suitable for a wide range of applications. 

DAC Stainless Steel HPLC Frit Filter

2. PEEK (Polyether Ether Ketone) Frits:

PEEK frits are made from a high-performance polymer known for its excellent chemical resistance and mechanical properties. These frits are particularly popular in modern HPLC systems due to their biocompatibility and compatibility with a broader range of solvents and pH conditions. PEEK frits are often used in HPLC columns designed for biomolecule analysis.

quality 50mm HPLC Pre Inline Filter Holder

Both types of frits serve the same fundamental purpose of retaining particles and contaminants while allowing the mobile phase and sample analytes to pass through for chromatographic separation. The choice between stainless steel and PEEK frits depends on factors such as the type of analysis, solvent compatibility, and the specific needs of the HPLC system. Regular maintenance and replacement of the frits are essential to ensure optimal performance and reliable results in HPLC analyses.

Why Column Frit HPLC is So Important ?

The column frit in HPLC (High-Performance Liquid Chromatography) is of utmost importance due to the following reasons:

  1. Particle Retention: The frit acts as a physical barrier that prevents particles and debris from entering the HPLC column. If these particles were to pass into the column, they could cause blockages, leading to chromatographic issues and affecting the quality of analytical results.

  2. Column Protection: By keeping contaminants away from the column packing material, the frit helps protect the integrity and longevity of the HPLC column. The column is a critical and often expensive component, so preserving its performance and lifespan is crucial for cost-effective and reliable analyses.

  3. Enhanced Efficiency: A properly functioning frit ensures a smooth flow of the mobile phase through the column while retaining the sample’s dissolved analytes. This efficient separation process allows for accurate identification and quantification of the compounds in the sample.

  4. Consistency and Reproducibility: A clean and well-maintained frit ensures consistent flow rates and pressure within the system, leading to reproducible results between different runs. Reproducibility is a fundamental requirement in analytical chemistry to validate the accuracy of the analysis.

  5. Minimized Downtime: Regularly replacing and maintaining the frit can help prevent unexpected column blockages during analysis, reducing downtime and the need for troubleshooting or column replacement.

  6. Sample Purity: The frit keeps larger particles and contaminants away from the column, ensuring that only the desired components of the sample are analyzed. This feature is especially crucial when dealing with complex samples that may contain impurities or other unwanted components.

  7. Sensitivity and Sensibility: A clean frit reduces the chance of interference with the analytes of interest, improving the sensitivity of the HPLC system to detect and quantify low-level compounds accurately.

  8. Analytical Accuracy: By preventing unwanted contaminants from entering the column, the frit helps maintain the accuracy of the analysis. This is essential in various applications, such as pharmaceutical quality control, environmental monitoring, and food safety analysis.

In summary, the HPLC column frit plays a vital role in maintaining the column’s performance, preserving sample integrity, and ensuring the accuracy and reproducibility of analytical results. By acting as a protective barrier and allowing only the desired analytes to pass through, the frit is a critical component in achieving reliable and high-quality chromatographic separations.

How to Change HPLC Frit Agilent ?

After You Get the New HPLC Frit, And Changing the frit in an Agilent HPLC column is a routine maintenance task that helps ensure the column’s optimal performance. Here We List a step-by-step guide on how to change the frit in an Agilent HPLC column:

Materials Needed:

  1. New frit (compatible with your Agilent HPLC column)
  2. PEEK tubing cutter or sharp scissors
  3. Tweezers or needle-nose pliers
  4. Analytical balance or ruler (to measure the correct length of the tubing)
 

Step-by-Step Procedure:

1.Prepare the Column:

* Remove the HPLC column from the system and set it on a clean and flat surface.

* Ensure that the column is empty and free from any solvent or sample.

2.Remove Old Frit:

* Using tweezers or needle-nose pliers, carefully grip the old frit at the column’s exit or entrance.

* Gently pull the old frit out of the column. Be cautious not to damage the column’s packing material while doing this.

3.Cut New PEEK Tubing:

Take the new PEEK frit and cut a small section of PEEK tubing with a PEEK tubing cutter or sharp scissors.

The length of the PEEK tubing should be slightly longer than the inner diameter of the column.

4.Insert New Frit:

Insert one end of the PEEK tubing into the column, ensuring that it goes all the way to the bottom.

Use tweezers or pliers to gently press the frit further into the column until it is securely seated.

5.Trim Excess Tubing:

If there is any excess PEEK tubing sticking out of the column, use the PEEK tubing cutter or sharp scissors to trim it so that it aligns with the column’s exit or entrance.

6.Reinstall the Column:

Once the new frit is securely in place, reinstall the column back into the HPLC system.

Ensure that all connections are tight and secure.

7.Flush the Column:

Before using the column for analysis, flush it with an appropriate solvent to remove any air bubbles and to condition the column.

It’s important to follow proper laboratory safety protocols while handling HPLC columns and equipment. Additionally, refer to the manufacturer’s instructions or user manual for your specific Agilent HPLC column model for any additional guidelines or recommendations regarding frit replacement.

Porous Metal Frits in Liquid Chromatography

HPLC frits are commonly used in the chromatography industry for a wide range of applications, including sample filtration and injection, column protection, and flow control. 

These small porous blocks, typically made from stainless steel or ceramic, serve as a filter for any unwanted particles or debris in the sample, ensuring optimal separation of the sample components during the chromatography process. Here are some common product applications for HPLC frit:

1. Sample filtration and injection – HPLC frits help remove particulate matter from samples before they are injected into the system.

2. Column protection – HPLC frits are used to prevent damage to the chromatography column by trapping unwanted debris.

3. Drug Discovery: HPLC is an essential tool for drug discovery research, as it provides a means for separating and analyzing complex mixtures of compounds. HPLC frits are used in a variety of drug discovery applications

4. Flow control – Frits are used to regulate the flow rate of the mobile phase and ensure optimal performance of the column.

5. Environmental Monitoring:  HPLC frits play a critical role in environmental monitoring, as they are used to separate and analyze a wide range of environmental contaminants. This helps to ensure that water and soil are safe for human consumption and other uses.

6. Sample preparation – HPLC frits are used in sample preparation to remove unwanted contaminants and ensure accuracy in subsequent testing.

7. Mass spectrometry – Frits are also used in mass spectrometry applications to prevent ions from entering the detector and interfering with results.

8. QA/QC Laboratories: HPLC frits are widely used in QA/QC laboratories to ensure the quality and safety of products. They are used to analyze a variety of samples, including pharmaceuticals, food, and cosmetics.

9. In-Vitro Diagnostics:  HPLC frits are also used in in-vitro diagnostic applications, where they are used to analyze blood, urine, and other biological samples. This helps to diagnose and monitor a variety of medical conditions, including cancer and infectious diseases.

Frequently Asked Questions

some questions about HPLC Frit you and others like to know

HPLC frits are small, porous disks that are used to filter and trap particles in chromatographic columns. They are typically made of stainless steel or ceramic materials and are used to protect the column from particulate matter that could damage the stationary phase.

Image of HPLC frit

* Functions of HPLC Frits:

  • Retain the packing material: The frit prevents the packing material from being swept away by the mobile phase during high-pressure chromatography. This ensures a stable and consistent separation bed within the column.
  • Prevent contamination: The frit filters out impurities and particles present in the sample or mobile phase, protecting the column and sensitive components of the HPLC system from damage.
  • Ensure even flow distribution: The porous structure of the frit promotes a uniform flow of the mobile phase through the column, leading to improved separation efficiency and resolution.
  • Maintain pressure: The frit helps to maintain a constant pressure within the column, ensuring optimal chromatographic performance.
  •  

* Materials used for HPLC Frits:

  • Stainless steel: The most common material for HPLC frits, offering high strength and chemical resistance.
  • PEEK (Polyether ether ketone): A good choice for high-pressure applications and when chemical resistance is crucial.
  • PTFE (Polytetrafluoroethylene): Inert and resistant to a wide range of chemicals, but not suitable for high pressures.
  • Porous metal: Provides excellent filtration efficiency and high flow rates.
 
 

* Selection of HPLC Frits:

Pore size: The pore size of the frit must be smaller than the particle size of the packing material to prevent particles from passing through.

  • Material: Choose a material compatible with the mobile phase and sample composition.
  • Pressure rating: Ensure the frit can withstand the pressures used in your HPLC system.
  • Size: Select the appropriate size frit for your column or filter.
  •  

* Installation and Maintenance of HPLC Frits:

  • Follow the manufacturer’s instructions for proper installation of the frit in the column or filter.
  • Clean the frit regularly to remove any accumulated contaminants that may affect the performance of the HPLC system.
  • Replace the frit when it becomes clogged or damaged.

In summary, HPLC frits are essential components of HPLC columns and filters, playing a vital role in achieving optimal chromatographic performance. By understanding their functions, materials, and selection criteria, you can ensure the efficient and reliable operation of your HPLC system.

HPLC frits are used in drug discovery to separate and analyze complex mixtures of compounds. By using HPLC frits, researchers can identify and isolate specific compounds of interest, which can then be further studied for potential therapeutic applications. and as we know, there are 3 main purpose, please check as following: 

HPLC frits fulfill several crucial purposes within the drug discovery process:

1. Separation and Analysis of Complex Mixtures:

Drug discovery involves identifying and isolating promising drug candidates from a vast pool of potential compounds. HPLC, with its high resolution and sensitivity, allows researchers to separate these complex mixtures and analyze individual components effectively. Frits play a vital role in this process by:

  • Ensuring consistent flow: They provide a stable platform for the packing material within the column, guaranteeing a uniform flow of the mobile phase and optimal separation of compounds.
  • Filtering impurities: They capture any contaminants or particles present in the sample or mobile phase, preventing them from interfering with the separation process and affecting the purity of isolated compounds.
  • Maintaining column integrity: They prevent the packing material from being swept away by the high-pressure mobile phase, protecting the column from damage and ensuring its longevity.
  •  

2. Identification and Characterization of Drug Candidates:

Once promising drug candidates are separated, frits help in their further characterization and evaluation:

  • Fraction collection: They facilitate the collection of specific fractions containing the desired compounds, enabling researchers to study their properties and activities in detail.
  • Purification: They can be used in purification steps to further refine and isolate the target compounds from other components present in the mixture.
  • Pharmacokinetic studies: Frits play a role in analyzing the behavior and efficacy of drug candidates within biological systems, aiding in identifying potential lead compounds for further development
  •  

3. Quality Control and Monitoring:

HPLC frits are crucial for maintaining quality control and monitoring throughout the drug discovery process:

  • Verification of purity: They help ensure the purity of drug candidates and other reagents used in the process, preventing contamination and ensuring the validity of experimental results.
  • Monitoring stability: They facilitate the analysis of drug candidates and their stability under various conditions, informing decisions regarding formulation and storage.
  • Troubleshooting and optimization: They can be used to identify and troubleshoot issues with the HPLC system or chromatographic separation process, allowing for optimization and improvement.

Overall, HPLC frits are invaluable tools in drug discovery, contributing to efficient compound separation, characterization, quality control, and advancement of promising drug candidates for further research and development.

HPLC frits are used in environmental monitoring to separate and analyze a wide range of environmental contaminants. By using HPLC frits, researchers can identify and quantify contaminants in water and soil samples, which helps to ensure that these resources are safe for human consumption and other uses.

HPLC frits are used in QA/QC laboratories to ensure the quality and safety of products. They are used to analyze a variety of samples, including pharmaceuticals, food, and cosmetics, to ensure that they meet specific quality standards and regulatory requirements.

HPLC frits are used in in-vitro diagnostic applications to analyze biological samples, such as blood and urine, for the presence of specific biomarkers. By using HPLC frits, researchers can diagnose and monitor a variety of medical conditions, including cancer and infectious diseases.

HPLC frits should be replaced on a regular basis to ensure the integrity of the column and the accuracy of the results. The frequency of replacement will depend on the specific application and the type of frit used, but in general, frits should be replaced every 50-100 injections.

Choosing the right HPLC frit depends on a variety of factors, including the sample matrix, the flow rate, and the column dimensions. It is important to consult with an expert in chromatography to determine the best frit for your specific application.

With some experience from our HPLC expert, The frequency of replacing HPLC frits varies depending on several factors, including:

* Type of frit: Different materials have different lifespans. Stainless steel frits typically last longer than PEEK or PTFE frits.

* Pressure used: High pressure can degrade frits faster than low pressure.

* Nature of samples and mobile phases: Samples with high particle content or aggressive mobile phases can clog frits quickly.

* Frequency of use: Columns used more frequently may require more frequent frit replacement.

General guidelines for frit replacement:

* Stainless steel frits: Every 6-12 months or when the system pressure increases significantly.

* PEEK frits: Every 3-6 months or when the system pressure increases significantly.

* PTFE frits: Every 1-3 months or when the system pressure increases significantly.

Here are some signs that your HPLC frit might need replacing:

* Increased system pressure: This is the most common sign of a clogged frit.

* Decreased peak resolution: A clogged frit can cause peaks to broaden and merge together.

* Change in peak shape: A distorted peak shape can indicate a partially blocked frit.

* Increased baseline noise: This can be caused by contamination from a damaged frit.

Here are some additional tips for extending the lifespan of HPLC frits:

* Use high-quality filters to remove particles from your samples and mobile phases.

* Flush your columns regularly with appropriate solvents.

* Avoid using harsh chemicals on your frits.

* Inspect your frits regularly for signs of damage.

Remember, replacing a frit is a relatively inexpensive procedure. It is better to replace a frit too often than to risk damaging your column or compromising your results.

Need Help ?

Contact uHPLCs Today for Any Questions for HPLC / UHPLC 

+(86) 0755-28502380

sales@uhplcs.com

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