Improve separations with precise columns.
HPLC Column Packing Material
Silica gel is the most widely uesd subdrate for Liquid Chromatography stationary phases. Its physical and chemical properties make it suitable for a wide variety of analytical and preparative separations.
Average particle size
1.8um、2um, 3um、5um、10um 20um、30umand more larger particle
HE Standard SealUn-Sealed
Average Pore Size
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Features & Benefits
> SPS purity SiO2 is higher than 99.99%
> Selection of particle sizes to optimize efficiency
> Tight distribution of particle sizes and spherical particle shape to reducr back-pressure
> Lot-to-Lot consistency for reproducible performance
> Superior pressure strength
> Consistent and reproducible retention times to allow direct scale-up from laboratory through process applications
> Large bulk quantities for all applications available, secutrd lots offered for process use
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HPLC Column FAQ
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HPLC Column Types: A Beginner’s Guide
Types of Columns Used in HPLC Why use HPLC Columns ? High-Performance Liquid Chromatography (HPLC)
Applications of HPLC Column Packing Material
HPLC column packing material is used in a wide range of applications in various industries, including pharmaceuticals, biotechnology, environmental testing, food and beverage analysis, and more. Here are some specific applications of HPLC column packing material:
Pharmaceutical industry: HPLC column packing material is used to separate and purify active pharmaceutical ingredients (APIs) and their impurities. This is crucial for ensuring the quality, safety, and efficacy of drugs.
Biotechnology industry: HPLC column packing material is used for protein and peptide purification. It can separate proteins and peptides based on their size, charge, and hydrophobicity.
Environmental testing: HPLC column packing material is used to analyze environmental samples such as water, soil, and air for the presence of pollutants and contaminants. It can separate and quantify a wide range of compounds, including pesticides, herbicides, and heavy metals.
Food and beverage analysis: HPLC column packing material is used to analyze food and beverage samples for the presence of additives, preservatives, and contaminants. It can also be used to quantify nutrients such as vitamins and amino acids.
Forensic science: HPLC column packing material is used for drug testing and toxicology analysis. It can separate and quantify drugs and their metabolites in biological samples such as blood, urine, and saliva.
Overall, HPLC column packing material plays a critical role in various analytical applications by providing high separation efficiency, reproducibility, and selectivity.
How It Works ?
Frequently Asked Questions
Here are some questions about HPLC Column Packing and Materials, Do you Have Any Other Questions for HPLC system? You are Welcome to Contact us by email for more details .
HPLC column packing material is a stationary phase material that is packed into a chromatography column to separate and purify different compounds from a mixture. The packing material is typically made up of small particles that are packed tightly into the column to maximize the separation efficiency.
There are several types of HPLC column packing materials available, including silica-based, polymer-based, and metal-based materials. Silica-based materials are the most commonly used and offer high separation efficiency, while polymer-based materials offer good chemical stability and selectivity. Metal-based materials are less commonly used but offer unique selectivity for certain compounds.
The choice of HPLC column packing material depends on the specific application and the compounds that need to be separated. Factors such as polarity, size, and chemical structure of the compounds should be considered when selecting the packing material.
HPLC column packing materials are designed to withstand high pressures and typically have a maximum pressure rating of up to 400 bar (5800 psi).
To pack an HPLC column with packing material, the column is first filled with a slurry of the packing material in a suitable solvent. The slurry is then allowed to settle, and the excess solvent is drained off. The column is then packed by applying pressure to the slurry to compact the packing material.
The frequency of packing material replacement depends on the specific application and the amount of sample processed. In general, it is recommended to replace the packing material every 6-12 months to ensure consistent and reliable performance.
Common problems associated with HPLC column packing material include poor column efficiency, loss of resolution, and column blockage. These problems can be caused by factors such as poor packing, incorrect particle size or composition, and column overloading. Regular column maintenance and proper packing techniques can help prevent these issues.
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