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USHD C18-Pro 5 µm 4.6 x 250 mm

Unlock unparalleled robustness with the USHD C18-Pro HPLC column, where superior chemical resistance ensures consistent, high-quality performance.

  • Exceptional pH Stability: Withstands prolonged exposure to 1 M NaOH and 1 M H2SO4, maintaining peak performance without measurable decrease.
  • Chemical Durability: Resistant to stripping, bleeding, or dissolving under any pH condition, for reliable and reproducible outcomes.
  • Temperature Resistant: Outperforms conventional silica C18 columns with an ability to operate at temperatures above 100°C.
  • Extended Column Life: Engineered for longevity, providing dependable results throughout its extended usage.

Embrace the strength and consistency of the USHD C18-Pro column, your ally in achieving precise analytical results.


Here’s the table details specification about the USHD C18-Pro 5 µm 4.6 x 250 mm HPLC column:

Separation ModeReversed-Phase
Stationary PhasePRP-C18
FormatHPLC Column
Hardware Inner Diameter4.6 mm
Hardware Length250 mm
Particle Size5 µm
USP ClassificationUSP L21
Additional specifications: 
Packing Material TypePS-DVB functionalized with C18
Pore Size100 Å
Particle Surface Area (m2/cm3)368
Pore Volume (cm3/g)0.624
Hardware MaterialStainless Steel
Max Pressure5,000 psi
Mobile Phase LimitspH 1-13, 0-100% aqueous, organic modifier
Buffer Strength0.0-0.5N
Temp Limits5-85°C
RestorationRun a gradient of 100% water to 100% acetonitrile. Repeat three times
Compound ClassificationOrganics
Stainless Steel Preparative HPLC Empty Column For Chemistry Lab Equipment

Column body

This is a measure of the distribution of particles packed in a column. In HPLC, it is preferable to have narrow particle size distribution as a narrow particle size distribution can lead to better HPLC column efficiency.


In LC chromatography the reaction of a silylating agent with unreacted accessible silanols remaining on the silica gel surface after the initial bonding reaction of the HPLC column stationary phase. HPLC column endcapping eliminates unwanted secondary silanol interactions between compounds and the stationary phase.

PEEK frit

Longer filtration life
Consistent frit-to-frit swept volumes
Biocompatible and inert to most solvents
Suitable for low and high pressure applications.
Enhanced sealing and excellent chemical resistance.
PEEK frits can be used up to 80 ºC.

Specification( Customizable)/ Size



USHD C18-Pro ×30mm ;  2.1×50mm ;  2.1×75mm  ; 2.1×100mm  ; 2.1×125mm  ; 2.1×150mm;  2.1×200mm 2.1×250mm 2.1×300mm

316L Stainless SteelAnalytical

3.0 ×30mm ; 3.0×50mm ;  3.0×75mm ; 3.0×100mm ; 3.0×125mm ; 3.0×150mm ; 3.0×200mm ;  3.0×250mm ;  3.0×300mm ;

316L Stainless SteelAnalytical

4.0×20mm ; 4.0×30mm ; 4.0×50mm ; 4.0×100mm

4.0×150mm ;   ;  4.0×200mm; 4.0×250mm ;  4.0×300mm

316L Stainless SteelAnalytical

4.6×20mm ; 4.6×30mm  ; 4.6×50mm ; 4.6×100mm

4.6×150mm   ; 4.6×200mm ; 4.6×250mm ; 4.6×300mm 

316L Stainless SteelAnalytical

7.8×150mm ; 7.8×250mm ;  7.8×300mm

316L Stainless SteelSemi-prep

10×50mm ; 10×100mm ;  10×150mm

10×200mm ; 10×250mm ; 10×300mm

316L Stainless SteelSemi-prep

20×50mm  ; 20×100mm ; 20×150mm  ;  20×200mm

20×250mm  ; 20×300mm

316L Stainless SteelSemi-prep

21.2×150mm ;  21.2× 250mm

316L Stainless SteelSemi-prep



Email: sales@uhplcs.com
Phone: +86 0755 28502380
51-3, Fu 'an West Road, Pinghu Street, Longgang District, Shenzhen City, 518111 China
We Will Send Back Your Inquiry Within 48-Hours

Collaborate with uHPLCs for Tailored Chromatography Solutions

Take your analytical capabilities to the next level by working hand-in-hand with uHPLCs’ dedicated R&D engineers to customize chromatography components for your specific needs.

Our “Custom Engineered” service connects you with the expertise of a uHPLCs R&D Engineer, who will meticulously assess your application requirements. Whether your analysis demands a standard approach or a bespoke solution, our team is equipped to provide a personalized answer to your chromatographic challenges.

  • Direct Engineer Engagement: Discuss your specific needs and challenges with a professional who understands the intricacies of chromatography.
  • Customized Solutions: Benefit from customized modifications tailored just for your applications, whether for unique separations or complex matrices.
  • Standard Solutions with a Twist: Optimize even our standard offerings to better suit your laboratory workflows and analytical objectives.

uHPLCs is committed to delivering not just products, but also solutions that enhance the precision and efficiency of your chromatographic analyses.

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