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C8 HPLC Column Wholesale

C8 HPLC Column Professional Supplier

uHPLCs is a premier supplier of C8 HPLC columns, renowned for delivering high-quality products with exceptional performance. As experts in HPLC columns, uHPLCs specializes in providing advanced chromatographic solutions tailored to meet the diverse needs of researchers and analysts. Our C8 columns are designed to offer superior selectivity, robustness, and efficiency, making them ideal for a wide range of analytical tasks. With a strong commitment to innovation and customer satisfaction, uHPLCs is your trusted partner for all your HPLC column needs.

Key Features:

  • Superior Selectivity: Designed for precise and accurate separations, ensuring reliable results.
  • High Efficiency: Provides excellent peak resolution and sharpness for thorough analysis.
  • Robust Performance: Durable columns that deliver consistent performance over extended use.
  • Versatility: Suitable for various applications, from pharmaceuticals to environmental analysis.
  • Advanced Technology: Utilizes state-of-the-art materials and manufacturing techniques for optimal performance.

Choose uHPLCs for your C8 HPLC column needs and experience the excellence in chromatographic solutions that sets us apart.

C8 Column Specification

Item USP Pore Size Option Surface area (m²/g) Carbon Load (%) PH Tolerance Range Column Specifications Features and Application
5μm, 150A
3.5μm, 150A
4.6X250mm; 4.6X150mm; 3.0X100mm; 3.0X50mm; 2.1X100mm; 2.1X50mm
1.) Unique bonding process, excellent batch reproducibility.
2.) Lower carbon content, higher efficiency separation capability.
3.) Double tail-capping process, strong stability, good performance in detecting acidic and alkaline compounds Good peak shape.
4.) Lower specific surface area, slightly larger pore size, enhanced anti-pollution, and higher The C8 column has significantly longer column life and better performance.
5.) Especially suitable for material separation applications where retention time is desired to be reduced.
USHD C8 Column
5μm, 120A
3μm, 120A
1.8μm, 120A
4.6X250mm; 4.6X150mm; 3.0X100mm; 3.0X50mm; 2.1X100mm; 2.1X50mm
1.) Unique bonding process, excellent batch reproducibility.
2.) Higher carbon content, higher column efficiency and selectivity, and stronger retention capacity.
3.) Double tail sealing process, strong stability.
HPLC Colun in the HPLC System Connect Diagram by uhplcs

In addition to custom columns, uHPLCs also offers a wide range of standard columns for various applications such as reversed-phase, normal-phase, ion exchange, size exclusion, and HILIC. We also offers prepacked columns and accessories, such as frits, end-fittings, and Guards Columns.

uHPLCs uses state-of-the-art manufacturing techniques to produce high-quality columns that are consistent, reliable, and provide excellent performance. we have a strict quality control program to ensure that all columns meet the highest CE , SGS and UL standards for performance and reproducibility.

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Experience the Precision and Reliability of Our High-Quality C18 HPLC Columns Today

WHY uHPLCs' C8 HPLC Column

uHPLCs is a company that specializes in high-performance liquid chromatography (HPLC) columns, and their C8 HPLC column is one of their most popular products. There are several reasons why you might choose a uHPLCs C8 HPLC column for your separations:

  • Superior selectivity: uHPLCs C8 columns are designed for precise and accurate separations, ensuring reliable results. This is important for a variety of applications, such as pharmaceutical analysis, environmental testing, and food science.
  • High efficiency: These columns provide excellent peak resolution and sharpness, which allows you to see even very closely spaced components in your sample. This is important for complex samples that contain many different compounds.
  • Robust performance: uHPLCs C8 columns are durable and can withstand high pressures and temperatures. This means that you can use them for a wide range of mobile phases and analytical conditions.
  • Versatility: C8 columns are a good all-purpose option for a wide variety of analytes, from hydrophobic to moderately polar. This makes them a good choice for laboratories that do a lot of different types of HPLC analysis.
  • Advanced technology: uHPLCs uses state-of-the-art materials and manufacturing techniques to create their columns. This ensures that you are getting a high-quality product that will perform well for many years.

Here are some additional things to consider when choosing a C8 HPLC column:

  • Particle size: Smaller particle sizes will typically provide higher resolution, but they will also result in higher back pressures. You will need to choose a particle size that is compatible with your HPLC instrument.
  • Pore size: The pore size of the column will affect how large the molecules can be that can be separated. You will need to choose a pore size that is appropriate for the size of the molecules in your sample.
  • Carbon load: The carbon load is the percentage of carbon chains that are bonded to the silica gel surface. A higher carbon load will result in a more hydrophobic column. You will need to choose a carbon load that is appropriate for the polarity of your analytes.

If you are not sure which C8 HPLC column is right for you, you can contact uHPLCs or another HPLC column supplier for assistance.

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C8 HPLC Column FAQ

Main Features of C8 HPLC Column ?

C8 HPLC columns are a type of reversed-phase high-performance liquid chromatography (HPLC) column that is commonly used for the separation of a wide range of analytes. They are known for their versatility and are a good starting point for method development. Here are some of the main features of C8 HPLC columns:

Significance of Endcapping in Columns HPLC
  • Moderately hydrophobic stationary phase:C8 columns have a stationary phase that is bonded with octylsilane groups. These groups are eight carbon atoms long, which makes them more hydrophobic than C18 columns (which have 18 carbon atoms) but less hydrophobic than C4 columns (which have four carbon atoms). This makes C8 columns a good choice for separating analytes with a wide range of hydrophobicities.

  • Good selectivity: C8 columns can provide good selectivity for a variety of analytes, including pharmaceuticals, biochemicals, and environmental samples. This is due to the interaction between the hydrophobic stationary phase and the analytes.

  • Compatibility with a wide range of mobile phases: C8 columns can be used with a wide range of mobile phases, including water, acetonitrile, and methanol. This makes them a versatile choice for a variety of HPLC applications.

  • Fast analysis times: Because C8 columns are moderately hydrophobic, they can provide faster analysis times than more hydrophobic columns, such as C18 columns.

  • High efficiency: C8 columns can be packed with very small particles (such as 3 or 5 microns), which can lead to high efficiency separations.

  • Relatively low cost: C8 columns are generally less expensive than some other types of HPLC columns, such as specialty columns.

Overall, C8 HPLC columns are a versatile and widely used type of HPLC column that can be used for a variety of applications. They are a good choice for analysts who are looking for a column that can provide good performance for a wide range of analytes.

Types of C8 HPLC Column and How Choose ?

There are various types of C8 HPLC columns available, each with slightly different properties that can affect the separation of your analytes.

Here are 3 popular C8 HPLC Column in the market :

1. Universal C8 columns :

These are the most general-purpose type of C8 column and are a good starting point for method development.

They have a balanced combination of hydrophobic and hydrophilic character, making them suitable for separating a wide range of analytes.

Image of Universal C8 HPLC Column

2.C8 bonded with end capped groups:

These columns have a special type of bonding group that reduces the activity of the silanol groups on the silica surface.

This can improve peak shape and reduce silanol interactions with some analytes.

Image of C8 bonded with endcapped groups HPLC Column
  • ** superficially porous C8 columns ( superficially ported particles (SPP)):These columns have a solid core with a porous outer layer. This design provides a high surface area for analyte interaction while reducing backpressure. This can be beneficial for high-throughput applications or when using long columns for high resolution separations.
    Image of superficially porous C8 HPLC Column 


3.Hydrophilic interaction liquid chromatography (HILIC) C8 columns :

These columns are a type of mixed-mode column that combines reversed-phase and hydrophilic interaction chromatography (HILIC) properties.

They can be useful for separating polar analytes that are difficult to separate with traditional reversed-phase columns.

Image of Hydrophilic interaction liquid chromatography (HILIC) C8 columns

Choosing the right type of C8 HPLC column for your application will depend on several factors, including:

*The properties of your analytes:

Consider the polarity, size, and charge of your analytes. C8 columns are generally a good choice for moderately hydrophobic analytes.

*The desired separation selectivity:

If you need to separate analytes based on specific functional groups, you may need to choose a C8 column with a specific bonded phase or endcapped group.

*The required resolution:

For high-resolution separations, you may need to use a column with a smaller particle size or a superficially porous particle (SPP) design.

*The desired analysis time:

Columns with larger particle sizes will generally have faster analysis times, but may not provide as high resolution.

*Compatibility with your mobile phase:

Make sure that the C8 column you choose is compatible with the mobile phase you plan to use.


It is always a good idea to consult with a chromatography expert or the manufacturer of the column to get recommendations for your specific application.


Applications of C8 HPLC Column

Till now , C8 HPLC columns shine in various analytical tasks due to their moderately hydrophobic nature.

Here are some of their key applications:

1.Separating moderately hydrophobic analytes:

C8 columns are the go-to choice for separating a wide range of analytes with moderate hydrophobicity.

This includes:

* Pharmaceuticals: Drugs, drug metabolites, and other pharmaceutical compounds.

*Biochemicals: Proteins, peptides, amino acids, and other biomolecules.

*Environmental samples: Pesticides, herbicides, and other environmental contaminants.

*Food and beverages: Analysis of food additives, flavors, and other components.


2.General-purpose separations:

As universal C8 columns offer a balanced hydrophobic and hydrophilic character,

they serve as a good starting point for method development in various analytical tasks.


3.Faster analysis times:

Compared to more hydrophobic columns like C18, C8 columns allow for faster analysis due to weaker interactions

between the stationary phase and analytes. This is beneficial for high-throughput workflows.


4.Biomolecule separations:

Some C8 columns, particularly those with wider pores and endcapped groups, are well-suited for separating

biomolecules like proteins and peptides. These features minimize unwanted interactions and improve peak shapes.


5.Complementary technique to C18:

If a C18 column retains your analytes too strongly, a C8 column can be a good alternative for achieving

better separation and faster elution times.


Here are some additional points to consider:

*HILIC applications:

Specific types of C8 columns, like HILIC C8, offer mixed-mode functionality. They combine reversed-phase and hydrophilic

interaction properties, making them useful for separating highly polar analytes that are challenging with traditional reversed-phase columns.


*Choice depends on specific needs:

The most suitable C8 column type for your application depends on factors like analyte properties, desired resolution,

and compatibility with your mobile phase. Consulting a chromatography expert or the column manufacturer is recommended

for tailored recommendations.


Frequently Asked Questions

As Follow is Some Frequently Questions People Asked

A: C8 columns offer several advantages, including:

  • Versatility for separating a wide range of analytes.
  • Good selectivity for various compounds.
  • Compatibility with diverse mobile phases.
  • Potential for faster analysis times compared to more hydrophobic columns.
  • Generally lower cost compared to some specialty columns.

A: Common types include:

  • Universal C8 (good starting point for method development).
  • Endcapped C8 (reduced silanol activity for improved peak shapes).
  • Superficially porous C8 (SPP) for high surface area and reduced backpressure.
  • HILIC C8 (mixed-mode for separating highly polar analytes).
  • A: The lifespan of a C8 HPLC column depends on various factors, including:
    • Frequency of use.
    • Mobile phase conditions (pH, harsh solvents).
    • Sample cleanliness.
    • Storage conditions.

Generally, C8 columns can last for hundreds to thousands of injections with proper care. However, performance can gradually degrade over time, leading to increased backpressure, reduced efficiency, and poor peak shapes. It’s crucial to monitor column performance and replace it when necessary.

A: Here are some tips to extend the life of your C8 HPLC column:

  • Use filtered mobile phases to minimize contamination.
  • Avoid harsh mobile phase conditions (extreme pH, strong solvents) whenever possible.
  • Inject clean samples free of particulates.
  • Store the column properly according to manufacturer’s recommendations (usually capped with plugs and in a cool, dry place).
  • Regularly flush the column with appropriate solvents to remove residual analytes.

A: In some cases, yes. You can attempt to regenerate a C8 column by washing it with solvents to remove strongly bound contaminants. However, the effectiveness of regeneration depends on the severity of the contamination. If the column performance doesn’t improve significantly, replacement might be necessary.

A: Most chromatography column manufacturers provide detailed information on their websites, including product specifications, recommended applications, and compatibility data. You can also consult chromatography textbooks or online resources for broader information about C8 columns and HPLC techniques.

Need Help ?

Contact uHPLCs Today for Any Questions for HPLC / UHPLC 

+(86) 0755-28502380


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