C8 HPLC Columns
USHD C8 HPLC Columns
USHD C8 is a reversed-phase octyl (C8) family, USP L7. The shorter chain retains less strongly than C18, which helps when a C18 method holds analytes too long.
C8-Pro is 150 Å, 7% carbon, in 5 μm and 3.5 μm. USHD C8 is 120 Å, 12% carbon, in 5 μm, 3 μm, 1.8 μm and 1.7 μm. Both are listed for pH 2–8.
Analytical HPLC column
Features
Two USP L7 octyl phases. C8-Pro is the lower-carbon, 150 Å option. USHD C8 is the higher-carbon, 120 Å option.
- USHD C8-Pro — 7% carbon, 200 m²/g, 150 Å, pH 2–8. Lower carbon and a larger pore than USHD C8. Listed for methods that need shorter retention.
- Double end-capping — Both phases are described with a double end-capping process for peak shape of acidic and basic compounds.
- USHD C8 — 12% carbon, 320 m²/g, 120 Å, pH 2–8. Higher carbon load, stronger retention and higher selectivity than C8-Pro.
- Particle sizes on the catalog — C8-Pro: 5 μm and 3.5 μm. USHD C8: 5 μm, 3 μm, 1.8 μm and 1.7 μm.
Product Selection
Choose C8-Pro or USHD C8, then confirm the catalog number in the selection table.
Specifications
Phase specifications for USHD C8-Pro and USHD C8.
| Phase | USP | Particle Size | Pore Size | Surface Area | Carbon Load | pH |
|---|---|---|---|---|---|---|
| USHD C8-Pro | L7 | 5 μm, 3.5 μm | 150 Å | 200 m²/g | 7% | 2–8 |
| USHD C8 | L7 | 5 μm, 3 μm, 1.8 μm, 1.7 μm | 120 Å | 320 m²/g | 12% | 2–8 |
USHD C8-Pro catalog
15 standard sizes. 4.6×250 mm is listed for 5 μm only.
| Product No. | Dimensions | Particle Size | Pore Size |
|---|---|---|---|
| P1F0B-00469 | 2.1×50 mm | 5 μm | 150 Å |
| P1F0B-00470 | 2.1×100 mm | 5 μm | 150 Å |
| P1F0B-00471 | 3.0×50 mm | 5 μm | 150 Å |
| P1F0B-00472 | 3.0×100 mm | 5 μm | 150 Å |
| P1F0B-00473 | 4.6×50 mm | 5 μm | 150 Å |
| P1F0B-00474 | 4.6×100 mm | 5 μm | 150 Å |
| P1F0B-00349 | 4.6×150 mm | 5 μm | 150 Å |
| P1F0B-00348 | 4.6×250 mm | 5 μm | 150 Å |
| P1F0B-00339 | 2.1×50 mm | 3.5 μm | 150 Å |
| P1F0B-00462 | 2.1×100 mm | 3.5 μm | 150 Å |
| P1F0B-00463 | 3.0×50 mm | 3.5 μm | 150 Å |
| P1F0B-00464 | 3.0×100 mm | 3.5 μm | 150 Å |
| P1F0B-00465 | 4.6×50 mm | 3.5 μm | 150 Å |
| P1F0B-00466 | 4.6×100 mm | 3.5 μm | 150 Å |
| P1F0B-00467 | 4.6×150 mm | 3.5 μm | 150 Å |
USHD C8 catalog
28 standard sizes, including 1.0 mm ID at 1.7 μm and 3.0×150 / 2.1×150 mm where shown.
| Product No. | Dimensions | Particle Size | Pore Size |
|---|---|---|---|
| P1F0B-00524 | 2.1×50 mm | 5 μm | 120 Å |
| P1F0B-00525 | 2.1×100 mm | 5 μm | 120 Å |
| P1F0B-00526 | 3.0×50 mm | 5 μm | 120 Å |
| P1F0B-00527 | 3.0×100 mm | 5 μm | 120 Å |
| P1F0B-00813 | 3.0×150 mm | 5 μm | 120 Å |
| P1F0B-00528 | 4.6×50 mm | 5 μm | 120 Å |
| P1F0B-00529 | 4.6×100 mm | 5 μm | 120 Å |
| P1F0B-00354 | 4.6×150 mm | 5 μm | 120 Å |
| P1F0B-00332 | 4.6×250 mm | 5 μm | 120 Å |
| P1F0B-00516 | 2.1×50 mm | 3 μm | 120 Å |
| P1F0B-00517 | 2.1×100 mm | 3 μm | 120 Å |
| P1F0B-00748 | 2.1×150 mm | 3 μm | 120 Å |
| P1F0B-00518 | 3.0×50 mm | 3 μm | 120 Å |
| P1F0B-00519 | 3.0×100 mm | 3 μm | 120 Å |
| P1F0B-00749 | 3.0×150 mm | 3 μm | 120 Å |
| P1F0B-00520 | 4.6×50 mm | 3 μm | 120 Å |
| P1F0B-00521 | 4.6×100 mm | 3 μm | 120 Å |
| P1F0B-00522 | 4.6×150 mm | 3 μm | 120 Å |
| P1F0B-00684 | 1.0×50 mm | 1.7 μm | 120 Å |
| P1F0B-00682 | 1.0×100 mm | 1.7 μm | 120 Å |
| P1F0B-00475 | 2.1×50 mm | 1.8 μm | 120 Å |
| P1F0B-00509 | 2.1×100 mm | 1.8 μm | 120 Å |
| P1F0B-00750 | 2.1×150 mm | 1.8 μm | 120 Å |
| P1F0B-00510 | 3.0×50 mm | 1.8 μm | 120 Å |
| P1F0B-00511 | 3.0×100 mm | 1.8 μm | 120 Å |
| P1F0B-00751 | 3.0×150 mm | 1.8 μm | 120 Å |
| P1F0B-00512 | 4.6×50 mm | 1.8 μm | 120 Å |
| P1F0B-00563 | 4.6×100 mm | 1.8 μm | 120 Å |
Applications
C8 is the intermediate reversed-phase choice between C18 and shorter-chain phases.
Shorter retention than C18
Octyl bonding is less hydrophobic than octadecyl bonding, so many analytes elute earlier. Useful when a C18 method holds peaks too long.
Moderately hydrophobic analytes
Pharmaceuticals, biochemicals and environmental samples that do not need the full retention of a high-carbon C18.
C8-Pro, 150 Å
Lower surface area and a larger pore. The published description is for methods that want reduced retention and better resistance to contamination.
USHD C8, 120 Å
Higher carbon load and surface area for stronger retention and selectivity within the C8 family, including 1.8 μm and 1.7 μm formats.
Related: USHD C18 HPLC columns · All HPLC columns.
Support
Include the catalog number if you already have it. Otherwise send phase, particle size and dimensions.
Catalog matching
We confirm P1F0B numbers against phase, dimensions and particle size before quoting.
C18 method conversion
If your current C18 method retains too strongly, send the column size and mobile phase for a C8 suggestion.
Custom dimensions
Sizes that are not in the catalog table can be requested as a custom packed column.
FAQ
Answers from the USHD C8 specifications and the current catalog list.
01 What is the difference between USHD C8-Pro and USHD C8?
USHD C8-Pro is 150 Å silica, 200 m²/g, 7% carbon, in 5 μm and 3.5 μm. USHD C8 is 120 Å silica, 320 m²/g, 12% carbon, in 5 μm, 3 μm, 1.8 μm and 1.7 μm. Both are USP L7 and listed for pH 2–8.
02 When should I use C8 instead of C18?
C8 is less hydrophobic than C18, so analytes usually elute earlier. Use it when a C18 column retains the sample too strongly or when a shorter run is required.
03 Which catalog number is the 4.6×250 mm C8 column?
USHD C8-Pro, 5 μm, 150 Å, 4.6×250 mm is P1F0B-00348. USHD C8, 5 μm, 120 Å, 4.6×250 mm is P1F0B-00332. The 3.5 μm C8-Pro and 3 μm C8 lists do not include a 4.6×250 mm size.
04 What is the 1.7 μm size?
Two USHD C8 catalog rows are 1.7 μm, 120 Å: P1F0B-00684 (1.0×50 mm) and P1F0B-00682 (1.0×100 mm). The other sub-2 μm sizes in this list are 1.8 μm.
05 Can other dimensions be made?
The table is the standard catalog. Other internal diameters and lengths can be requested as a custom column.
Need a size matched to your method?
Request a USHD C8 Quote
Send the catalog number, or the phase, dimensions and particle size. We will confirm availability and pricing.
Helpful to include:
- Catalog number, or phase and dimensions
- Particle size (5 / 3.5 / 3 / 1.8 / 1.7 μm)
- Current C18 or C8 brand, if replacing one
- Quantity and destination
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