Size Exclusion HPLC Columns
USHD SEC HPLC Columns
USHD SEC columns separate macromolecules by size: proteins, antibodies, nucleic acids, peptides, polymers and biological drugs. The packing is high-purity silica, with the pore size chosen for the molecular-weight range.
USHD SEC-Bio is the size-exclusion phase, USP L33 / L59, pH 2–8, including a 1.8 μm option. USHD C8-Bio and USHD C4-Bio are hybrid phases for large molecules at pH 1–11.
USHD SEC-Bio
Features
Size-exclusion columns for molecular-weight and biomolecule work, plus two wide-pH hybrid phases for large molecules.
- USHD SEC-U — For water-soluble polymers, PEG, polysaccharides and other hydrophilic macromolecules. The current page also lists globular proteins from 100 Da to 3,500,000 Da. Particle size, pore size, dimensions and catalog numbers are not in the specification table.
- USHD SEC-Bio — USP L33 / L59, pH 2–8. Listed at 5 μm / 120 Å, 5 μm / 300 Å and 3 μm / 300 Å, in 4.6×300 mm and 7.8×300 mm.
- Low adsorption — The published SEC-Bio note is a bonding process that reduces dead adsorption, with stable batch-to-batch reproducibility.
- USHD SEC-Bio 1.8 μm — Same USP class and pH 2–8, at 1.8 μm and 300 Å. Listed in 4.6×150 mm and 4.6×300 mm for higher-resolution biological work.
- USHD C8-Bio and C4-Bio — Hybrid silica, 5 μm, 300 Å, 110 m²/g, pH 1–11. C8-Bio is 6% carbon (USP L7). C4-Bio is 4% carbon (USP L26).
Product Selection
SEC-U is the polymer phase. SEC-Bio is the biomolecule size-exclusion phase. C8-Bio and C4-Bio are the wide-pH hybrid phases.
USHD SEC-U
Polymers and hydrophilic samples
Water-soluble polymers, PEG and polysaccharides
View notes →
USHD SEC-Bio
Size exclusion, 120 Å and 300 Å
5 μm and 3 μm · pH 2–8 · 4.6×300 and 7.8×300 mm
View catalog →
Specifications
Figures from the current SEC column specification table. Surface area and carbon load are published only for C8-Bio and C4-Bio.
| Phase | USP | Particle size | Pore size | Surface area | Carbon load | pH | Dimensions |
|---|---|---|---|---|---|---|---|
| USHD SEC-Bio | L33 / L59 | 5 μm; 3 μm | 120 Å (5 μm); 300 Å (5 μm and 3 μm) | — | — | 2–8 | 4.6×300 mm; 7.8×300 mm |
| USHD SEC-Bio 1.8 μm | L33 / L59 | 1.8 μm | 300 Å | — | — | 2–8 | 4.6×150 mm; 4.6×300 mm |
| USHD C8-Bio | L7 | 5 μm | 300 Å | 110 m²/g | 6% | 1–11 | 4.6×150 mm; 4.6×250 mm |
| USHD C4-Bio | L26 | 5 μm | 300 Å | 110 m²/g | 4% | 1–11 | 4.6×150 mm; 4.6×250 mm |
USHD SEC-U
Listed on the current page for routine testing of water-soluble polymers, PEG, polysaccharides and other hydrophilic macromolecules, and for globular proteins from 100 Da to 3,500,000 Da. The specification table and the selection sheet do not give a particle size, pore size, dimension or catalog number for SEC-U. Include the sample molecular-weight range when you request a quote.
USHD SEC-Bio catalog
Six standard sizes from the selection sheet. 120 Å is listed at 5 μm only. 300 Å is listed at 5 μm and 3 μm.
| Catalog No. | Dimension | Particle size | Pore size |
|---|---|---|---|
| P1F0B-00487 | 4.6×300 mm | 5 μm | 120 Å |
| P1F0B-00307 | 7.8×300 mm | 5 μm | 120 Å |
| P1F0B-00489 | 4.6×300 mm | 5 μm | 300 Å |
| P1F0B-00340 | 7.8×300 mm | 5 μm | 300 Å |
| P1F0B-00484 | 4.6×300 mm | 3 μm | 300 Å |
| P1F0B-00485 | 7.8×300 mm | 3 μm | 300 Å |
USHD SEC-Bio 1.8 μm
1.8 μm, 300 Å, pH 2–8, in 4.6×150 mm and 4.6×300 mm. A catalog number is not on the selection sheet. Name the phase and dimensions when you request a quote.
USHD C8-Bio
USP L7. Hybrid silica bonded as a C8 phase, 5 μm, 300 Å, 110 m²/g, 6% carbon, pH 1–11. Dimensions: 4.6×150 mm and 4.6×250 mm. Published globular-protein range: 5,000–1,250,000.
USHD C4-Bio
USP L26. Hybrid silica bonded as a C4 phase, 5 μm, 300 Å, 110 m²/g, 4% carbon, pH 1–11. Dimensions: 4.6×150 mm and 4.6×250 mm. Published globular-protein range: 5,000–1,250,000.
Applications
Uses already described on this page: size-based analysis of biomolecules, and large-molecule work on the hybrid phases.
Molecular weight
Molecular-weight distribution of macromolecules, including proteins and water-soluble polymers.
Antibodies and ADCs
Antibody and ADC quality control, including the 1.8 μm SEC-Bio option for mAb purity and aggregate detection.
Biopharmaceutical R&D
Proteins, nucleic acids, enzymes, peptides and monoclonal antibody development on USHD SEC-Bio.
Protein aggregation
Aggregation and size-distribution checks, where larger species elute ahead of the monomer.
Polymers
Water-soluble polymer testing, listed among the typical uses on the current page.
Wide-pH samples
C8-Bio and C4-Bio when the method needs pH 1–11 and a 300 Å hybrid phase.
Related reversed-phase pages: USHD C18 HPLC columns · USHD C8 HPLC columns · All HPLC columns.
Support
Send the phase, particle size, pore size and dimensions. If you already have a catalog number, include it.
Phase selection
SEC-Bio for size exclusion at pH 2–8. C8-Bio or C4-Bio when the method needs the pH 1–11 hybrid phases.
Catalog confirmation
USHD SEC-Bio catalog numbers are in the table above. 1.8 μm, C8-Bio and C4-Bio sizes are confirmed with the quote.
Custom sizes
The listed dimensions are the standard set. Other internal diameters and lengths can be requested as a custom column.
FAQ
Answers from the current specification table and the USHD SEC-Bio selection sheet.
01 What is the difference between HPLC and SEC?
HPLC is the instrument method. SEC is one HPLC mode: molecules separate by size in a porous packing. Larger species enter fewer pores and elute earlier. Reversed phase, normal phase and ion exchange separate by interaction with the stationary phase, not by size alone.
02 Which phases are on this page?
USHD SEC-U, for water-soluble polymers and a published globular-protein range of 100–3,500,000 Da. USHD SEC-Bio and USHD SEC-Bio 1.8 μm, USP L33 / L59, pH 2–8. USHD C8-Bio (USP L7) and USHD C4-Bio (USP L26), both pH 1–11. Only SEC-Bio has catalog numbers on the selection sheet.
03 Which catalog numbers are listed for USHD SEC-Bio?
5 μm, 120 Å: P1F0B-00487 (4.6×300 mm) and P1F0B-00307 (7.8×300 mm). 5 μm, 300 Å: P1F0B-00489 (4.6×300 mm) and P1F0B-00340 (7.8×300 mm). 3 μm, 300 Å: P1F0B-00484 (4.6×300 mm) and P1F0B-00485 (7.8×300 mm).
04 What particle sizes and pore sizes are listed?
USHD SEC-Bio: 5 μm at 120 Å, 5 μm at 300 Å, and 3 μm at 300 Å. USHD SEC-Bio 1.8 μm: 1.8 μm, 300 Å. USHD C8-Bio and USHD C4-Bio: 5 μm, 300 Å. Surface area and carbon load are not published for the SEC-Bio phases.
05 What is the pH range?
USHD SEC-Bio and USHD SEC-Bio 1.8 μm are listed for pH 2–8. USHD C8-Bio and USHD C4-Bio are listed for pH 1–11.
06 What is USHD SEC-Bio used for?
Macromolecular biological samples: proteins, nucleic acids, antibodies, enzymes, peptides and ADC drugs, including monoclonal antibody development. The published notes are extremely low adsorption, stable batch reproducibility, and a bonding process intended to reduce dead adsorption.
07 When is the 1.8 μm SEC-Bio column used?
It is the higher-resolution SEC-Bio option, listed at 1.8 μm and 300 Å, in 4.6×150 mm and 4.6×300 mm. The current page points it at high-precision biological work such as mAb purity and aggregate detection. A catalog number is not on the selection sheet; include the dimensions in the quote request.
08 How do C8-Bio and C4-Bio differ?
Both use ultra-high-purity organic-inorganic hybrid macroporous silica, 5 μm, 300 Å, 110 m²/g, pH 1–11, in 4.6×150 mm and 4.6×250 mm. The published globular-protein range is 5,000–1,250,000. C8-Bio is USP L7 with 6% carbon. C4-Bio is USP L26 with 4% carbon.
09 When should an SEC column be replaced?
Replace it when resolution drops, backpressure rises, or peak shape no longer matches a known sample. Lifetime depends on sample cleanliness, mobile phase and storage. The published SEC-Bio note is that the bonding process is intended to reduce dead adsorption and extend column life.
Need a size matched to your method?
Request a USHD SEC Quote
Tell us the phase, pore size, particle size and dimensions. For USHD SEC-Bio, the catalog number is enough.
Helpful to include:
- Phase (SEC-U, SEC-Bio, SEC-Bio 1.8 μm, C8-Bio, C4-Bio)
- Catalog number, if it is a listed SEC-Bio size
- Particle size, pore size and dimensions
- Quantity and destination
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