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PFP Column

HPLC PFP Column Professional Supplier

uHPLCs is a leading supplier of HPLC PFP (Pentafluorophenyl) columns, known for delivering high-quality products and exceptional performance. 

As an HPLC Column expert, uHPLCs specializes in advanced chromatographic solutions tailored to the diverse needs of researchers and analysts. Our PFP columns provide superior selectivity, robustness, and efficiency, making them perfect for complex separations and challenging analytical tasks. With a strong commitment to innovation and customer satisfaction, uHPLCs is a trusted partner for all your HPLC column needs.

Key Features:

  • Superior Selectivity: Designed for complex separations, providing precise and accurate results.
  • High Efficiency: Offers excellent peak resolution and sharpness, ensuring reliable analysis.
  • Robust Performance: Durable columns that maintain performance over extended use.
  • Versatility: Suitable for a wide range of applications, from pharmaceuticals to environmental analysis.
  • Advanced Technology: Utilizes cutting-edge materials and manufacturing techniques for optimal performance.

Reverse Phase PFP HPLC Column

uHPLCS High Performance Liquid Chromatography Column


PFP Column Specification

Item USP Pore Size Option Surface area (m²/g) Carbon Load (%) PH Tolerance Range Column Specifications Features and Application
5μm, 120A
3μm, 120A
1. Ultra-pure silica matrix, bonded pentahelium phenyl stationary phase.
5μm, 120A
3μm, 120A
2. There are multiple functions of hydrophobicity, ion exchange, π-π, and Hilic during the analysis and detection process. model.
5μm, 120A
3μm, 120A
3. Suitable for separating aromatic hydrocarbons, halogen-containing compounds, and protonated amine compounds Quaternary ammonium compounds and polar metabolites, positively charged groups, including various isomers body, nitrogen-containing structural compounds in biopharmaceuticals, natural products, and environmental analysis use.
5μm, 120A
3μm, 120A
HPLC Colun in the HPLC System Connect Diagram by uhplcs

In addition to custom columns, uHPLCs also offers a wide range of standard columns for various applications such as reversed-phase, normal-phase, ion exchange, size exclusion, and HILIC. We also offers prepacked columns and accessories, such as frits, end-fittings, and Guards Columns.

uHPLCs uses state-of-the-art manufacturing techniques to produce high-quality columns that are consistent, reliable, and provide excellent performance. we have a strict quality control program to ensure that all columns meet the highest CE , SGS and UL standards for performance and reproducibility.

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uHPLCs understand that choosing the right HPLC column is crucial for achieving optimal results in your lab. That’s why we offer not just high-quality products, but also exceptional customer service.

Expert Guidance Every Step of the Way:

Our knowledgeable and experienced technical support team is always available to answer your questions and provide expert advice. They’ll guide you through the selection process, ensuring you find the perfect column for your specific application.

More Than Just Products:

uHPLCs is a professional and reliable HPLC column OEM factory located in China. We take pride in our commitment to quality, offering a wide range of standard products alongside the ability to customize columns to your exact needs. This flexibility allows us to cater to a diverse clientele within the HPLC field.

Some Reasons Why Choose uHPLCs’ PFP HPLC Columns: 

  • 1. Unique separation: PFP columns offer different selectivity than common C18 columns, which can be useful when C18 isn’t giving the separation you need .
  • 2. Water compatible: They can be used with 100% water mobile phases, making them a good option for analyzing water-soluble compounds.
  • 3. Strong retention for specific types of molecules: PFP columns can be good for separating halogenated compounds, isomers, and basic compounds.
  • 4. Durable: PFP columns are known for their good stability and long column lifetime.

Let uHPLCs be your partner in achieving superior HPLC results. Contact us today!

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C18 HPLC Column FAQ

Why Use C18 PFP HPLC Column ?

C18 PFP (Pentafluorophenyl) HPLC columns offer a unique combination of characteristics that make them highly effective for a wide range of analytical applications. Here are some key reasons to use C18 PFP HPLC columns:

  1. Enhanced Selectivity:

    • The combination of C18 and PFP functionalities provides a distinct selectivity profile. This dual interaction mechanism allows for better separation of complex mixtures, especially those containing aromatic compounds and halogenated molecules.
  2. Versatility:

    • C18 PFP columns are versatile and can handle a broad spectrum of compounds, including polar, non-polar, and moderately polar analytes. This makes them suitable for diverse applications across pharmaceuticals, environmental testing, and food analysis.
  3. Improved Peak Shape:

    • The unique interaction properties of the C18 and PFP groups lead to improved peak shapes and reduced tailing for both polar and non-polar compounds. This results in more accurate and reliable quantification.
  4. Strong Retention of Polar Compounds:

    • PFP groups enhance the retention of polar compounds, which can be challenging to separate using traditional C18 columns alone. This feature is particularly useful for analyzing complex samples with a mix of polar and non-polar substances.
  5. Stability and Durability:

    • C18 PFP columns are known for their robustness and longevity. They maintain performance over extended use and are resistant to a wide range of mobile phase conditions, ensuring consistent and reliable results.
  6. Compatibility with Diverse Mobile Phases:

    • These columns are compatible with a variety of mobile phases, including aqueous and organic solvents. This flexibility allows for optimized method development and tailored separation conditions.
  7. Reduction of Matrix Effects:

    • The unique selectivity of C18 PFP columns helps in minimizing matrix effects, leading to cleaner baselines and more accurate identification and quantification of target analytes.

So When choosing C18 PFP HPLC columns, analysts benefit from enhanced separation capabilities, improved analytical performance, and the versatility needed to tackle a wide array of challenging applications.

How Choose PFP HPLC Column

Some different factors you should consider when choosing PFP HPLC Column:

  • Particle size: Smaller particles (like sub-2 micron) can provide higher resolution but also result in higher back pressure. A larger particle size (like 5 micron) will be lower pressure but may not give as good of a separation.
  • Carbon load: The amount of bonded phase on the silica particle. A higher carbon load will generally result in more retention, but also can cause increased peak tailing.
  • Pore size: The size of the pores in the silica particles. A larger pore size will allow for the separation of larger molecules.

But these are just some of the things to consider when choosing a PFP HPLC column. The best column for your particular application will depend on the specific needs of your separation.

Some Applications of C18 HPLC Column

High-Performance Liquid Chromatography (HPLC) is a versatile analytical technique with a wide range of applications in various industries. The choice of HPLC column depends on the specific analytes and separation requirements of each application. Here are some common applications and the recommended HPLC column types for each:

  1. Pharmaceutical Analysis:

    • Application: Analyzing drugs, active pharmaceutical ingredients (APIs), impurities, and formulation components.
    • Recommended HPLC Column: C18 column (Reverse Phase). C18 is the most commonly used column for pharmaceutical analysis due to its broad applicability for separating a wide range of compounds.
  2. Environmental Analysis:

    • Application: Monitoring environmental pollutants, pesticides, herbicides, and other contaminants in water, soil, and air samples.
    • Recommended HPLC Column: C18 column (Reverse Phase) or HILIC column. The choice depends on the hydrophobicity/polarity of the target compounds. HILIC columns are suitable for polar analytes.
  3. Food and Beverage Analysis:

    • Application: Determining food additives, preservatives, vitamins, and other components in food and beverage samples.
    • Recommended HPLC Column: C18 column (Reverse Phase). C18 is widely used in food analysis due to its ability to separate a variety of food-related compounds.
  4. Biochemical Analysis and Proteomics:

    • Application: Identifying and quantifying biomolecules, such as peptides, proteins, amino acids, nucleic acids, and metabolites.
    • Recommended HPLC Column: C18 column (Reverse Phase) or C4 column. C18 is commonly used for peptides and proteins, while C4 is preferred for small polar compounds.
  5. Clinical and Forensic Toxicology:

    • Application: Detecting and quantifying drugs, metabolites, and toxins in biological samples for medical and forensic purposes.
    • Recommended HPLC Column: C18 column (Reverse Phase). C18 is suitable for analyzing a wide range of drugs and metabolites.
  6. Natural Product Analysis:

    • Application: Characterizing and identifying compounds from plant extracts and natural products.
    • Recommended HPLC Column: Depending on the complexity of the sample, C18 column (Reverse Phase) or C30 column. C30 provides additional hydrophobic interactions for complex mixtures.
  7. Chiral Separations:

    • Application: Separating enantiomers (optical isomers) of chiral compounds.
    • Recommended HPLC Column: Chiral column. Chiral columns have immobilized chiral selectors that differentiate enantiomers based on their stereoisomeric configurations.
  8. Pharmaceutical Preparative Purification:

    • Application: Isolating and purifying larger quantities of drugs and intermediates for drug development and manufacturing.
    • Recommended HPLC Column: Preparative C18 column (Reverse Phase) or other preparative columns with larger dimensions for increased loading capacity.

For each application, it is essential to consider the sample characteristics, the nature of the analytes, and the separation requirements to choose the most suitable HPLC column. Method development and optimization may also be necessary to achieve the desired results.

Frequently Asked Questions

As Follow is Some Frequently Questions People Asked

A PFP column in High Performance Liquid Chromatography (HPLC) refers to a type of reversed-phase column that features a pentafluorophenyl group as its stationary phase. The PFP stationary phase provides unique selectivity compared to conventional C18 columns due to the presence of electronegative fluorine atoms. These columns are particularly effective for separating compounds that have aromatic rings, polar functional groups, and halogenated compounds. The unique interactions in PFP columns include π-π interactions, dipole-dipole interactions, and hydrophobic interactions, making them versatile for various analytical applications.

PFP columns offer several advantages over traditional reversed-phase columns, including:

  • Enhanced Selectivity: The pentafluorophenyl phase provides unique selectivity for aromatic compounds and those with electron-withdrawing groups.
  • Improved Resolution: Due to the combination of π-π interactions and dipole-dipole interactions, PFP columns can achieve better resolution for closely related compounds.
  • Versatility: Suitable for a wide range of compounds, including pharmaceuticals, pesticides, and environmental samples.
  • Stability: PFP columns exhibit good chemical stability and can be used with a variety of mobile phases, including acidic and basic conditions.

While both PFP and C18 columns are used in reversed-phase HPLC, they differ significantly in their selectivity and interaction mechanisms:

  • C18 Columns: Primarily rely on hydrophobic interactions and are effective for non-polar to moderately polar compounds. They provide broad application but may struggle with compounds that have strong π-π interactions or polar functional groups.
  • PFP Columns: Offer additional interactions (π-π, dipole-dipole) and are particularly effective for polar compounds, aromatic compounds, and those with halogen atoms. They can resolve compounds that are difficult to separate on C18 columns.

PFP columns are particularly suited for:

  • Aromatic Compounds: Due to strong π-π interactions.
  • Halogenated Compounds: Enhanced separation due to the electronegativity of fluorine atoms.
  • Compounds with Polar Functional Groups: Dipole-dipole interactions improve separation.
  • Pharmaceuticals: Especially those with complex structures requiring specific selectivity.
  • Environmental Samples: Pesticides, herbicides, and other pollutants with varying functional groups.

PFP columns are versatile in terms of mobile phase compatibility. They can be used with:

  • Aqueous Solvents: Water, buffers.
  • Organic Solvents: Acetonitrile, methanol.
  • Additives: Can tolerate acidic and basic additives such as formic acid, trifluoroacetic acid, and ammonium acetate.
  • Gradient Elution: Suitable for gradient methods, allowing for the separation of complex mixtures.

Proper maintenance of a PFP column involves:

  • Regular Washing: Use appropriate solvents to wash the column after each use to prevent build-up of residues.
  • Storage: Store the column in a suitable solvent, typically acetonitrile or methanol, to prevent drying out.
  • pH Range: Operate within the recommended pH range (typically 2-8) to avoid damaging the stationary phase.
  • Temperature Control: Avoid exceeding the maximum temperature limit specified by the manufacturer.

Both are reversed-phase columns, but C18 has a longer alkyl chain (18 carbons) compared to C8 (8 carbons). This makes C18 more hydrophobic, leading to stronger interactions with analytes and longer retention times. C8 columns are preferred for separating more polar compounds that wouldn’t retain well on C18.

C18 vs. C8 HPLC Columns

Yes, PFP columns are excellent for method development due to their unique selectivity. They provide different separation characteristics compared to C18 columns, allowing for the optimization of methods for compounds that are challenging to separate. During method development, it’s essential to explore various mobile phase compositions and gradients to achieve the best resolution and selectivity.

While PFP columns offer many benefits, there are some limitations to consider:

  • Sample Compatibility: Not all compounds may benefit from the unique selectivity of PFP columns. For some non-aromatic, non-polar compounds, a C18 column might be more suitable.
  • Cost: PFP columns can be more expensive than conventional C18 columns due to the specialized stationary phase.
  • Method Development Time: It may take additional time to develop and optimize methods to fully utilize the advantages of PFP columns.

The choice between a PFP and a C18 column depends on the nature of the analytes and the specific requirements of the analysis:

  • Analyte Characteristics: Consider the functional groups, aromaticity, and polarity of the analytes.
  • Separation Goals: If enhanced selectivity and resolution for aromatic and polar compounds are needed, a PFP column may be more suitable.
  • Method History: If you have established methods using C18 columns and they meet your requirements, sticking with C18 may be practical. However, for new methods or challenging separations, exploring PFP columns could be beneficial.

PFP columns are used in various applications, including:

  • Pharmaceutical Analysis: For separating and analyzing complex drug formulations.
  • Environmental Analysis: For detecting and quantifying pollutants, pesticides, and herbicides.
  • Food and Beverage Testing: For identifying additives, contaminants, and natural compounds.
  • Forensic Science: For analyzing drugs, toxins, and other substances in biological samples.

Need Help ?

Contact uHPLCs Today for Any Questions for HPLC / UHPLC 

+(86) 0755-28502380


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