USHD C8-Bio HPLC Column (Macroporous, pH-Resistant for Biomolecular Analysis)
USHD C8-Bio HPLC Column is designed with ultra-high purity organic-inorganic hybrid macroporous silica gel and a proprietary bonding process for excellent stability and batch-to-batch reproducibility. Engineered for biopharmaceutical applications, it offers exceptional resistance to both strong acids and alkalis (pH 1–11), making it ideal for the analysis of proteins, peptides, ADC drugs, and monoclonal antibodies (mAbs).
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Features:
- Hybrid Macroporous Silica Matrix: Delivers high column efficiency with strong structural stability.
- Proprietary C8 Bonding Technology: Ensures reliable performance and consistent separation quality.
- Wide pH Tolerance (1–11): Excellent chemical resistance to strong acids and bases, suitable for demanding biological applications.
- Ideal for Macromolecular Detection: Tailored for proteins, peptides, ADC drugs, and mAb molecular weight range analysis.
- Excellent Batch Reproducibility: Stable results across production lots for consistent method development.
- Molecular Weight Range: Suitable for globular proteins between 5,000 – 1,250,000 Da.
Specifications
| Property | USHD C8-Bio HPLC Column |
|---|---|
| USP | L7 |
| Pore Size Option | 5µm, 300 Å |
| Surface Area (m²/g) | 110 |
| Carbon Load (%) | 6 |
| pH Tolerance Range | 1-11 |
| Column Specifications | 4.6× 250 mm 4.6 ×150 mm |
| Features and Applications |
|
Analytical column, C18 bonded phase, C18 chromatography, C18 column, C18 HPLC column, C18 stationary phase, Chromatography column, Column packing material, High performance liquid chromatography, HPLC analysis, HPLC column, HPLC separation, Liquid chromatography column, Reverse phase column, Silica-based column
Column body
This is a measure of the distribution of particles packed in a column. In HPLC, it is preferable to have narrow particle size distribution as a narrow particle size distribution can lead to better HPLC column efficiency.
Endcap
In LC chromatography the reaction of a silylating agent with unreacted accessible silanols remaining on the silica gel surface after the initial bonding reaction of the HPLC column stationary phase. HPLC column endcapping eliminates unwanted secondary silanol interactions between compounds and the stationary phase.
PEEK frit
Longer filtration life
Consistent frit-to-frit swept volumes
Biocompatible and inert to most solvents
Suitable for low and high pressure applications.
Enhanced sealing and excellent chemical resistance.
PEEK frits can be used up to 80 ºC.
All Hot Sale HPLC Columns
Select ideal HPLC column for your lab applications based on factors like separation efficiency, pore size, pH tolerance, and specifications.
| Item | USP | Pore Size Option | Surface area (m²/g) | Carbon Load (%) | PH Tolerance Range | Column Specifications |
|---|---|---|---|---|---|---|
|
USHD C18-Pro |
L1 |
5μm, 150A 3.5μm, 150A |
200 |
10 |
2-8 |
4.6X250mm 4.6X150mm 4.6X100mm 4.6X50mm 3.0X100mm 3.0X50mm 2.1X100mm 2.1X50mm |
|
USHD C18 |
L1 |
5μm, 120A 3μm, 120A 1.8μm, 120A |
320 |
17 |
2-8 |
4.6X250mm 4.6X150mm 4.6X100mm 4.6X50mm 3.0X100mm 3.0X50mm 2.1X100mm 2.1X50mm |
|
USHD C18-AQ |
L1 |
5μm, 120A 3μm, 120A 1.8μm, 120A |
320 |
12 |
2-8 |
4.6X250mm 4.6X150mm 4.6X100mm 4.6X50mm 3.0X100mm 3.0X50mm 2.1X100mm 2.1X50mm |
|
USHD C18-AR |
L1 |
5μm, 120A 3μm, 120A 1.8μm, 120A |
320 |
10 |
0.5-8 |
4.6X250mm 4.6X150mm 4.6X100mm 4.6X50mm 3.0X100mm 3.0X50mm 2.1X100mm 2.1X50mm |
|
USHD C18-T |
L1 |
5μm, 120A 3μm, 120A 1.8μm, 120A |
320 |
14 |
1-11 |
4.6X250mm 4.6X150mm 4.6X100mm 4.6X50mm 3.0X100mm 3.0X50mm 2.1X100mm 2.1X50mm |
|
USHD C8-PRO |
L7 |
5μm, 150A 3.5μm, 150A |
200 |
7 |
2-8 |
4.6X250mm 4.6X150mm 4.6X100mm 4.6X50mm 3.0X100mm 3.0X50mm 2.1X100mm 2.1X50mm |
|
USHD C8 |
L7 |
5μm, 150A 3.5μm, 120A 1.8μm, 120A |
320 |
12 |
2-8 |
4.6X250mm 4.6X150mm 4.6X100mm 4.6X50mm 3.0X100mm 3.0X50mm 2.1X100mm 2.1X50mm |
Analytical and Semi-Prep HPLC Column Size Option
Specification（ Customizable）/ Size | Materials | Applications |
USHD C18-Pro ×30mm ; 2.1×50mm ; 2.1×75mm ; 2.1×100mm ; 2.1×125mm ; 2.1×150mm; 2.1×200mm ; 2.1×250mm ; 2.1×300mm | 316L Stainless Steel | Analytical |
3.0 ×30mm ; 3.0×50mm ; 3.0×75mm ; 3.0×100mm ; 3.0×125mm ; 3.0×150mm ； 3.0×200mm ; 3.0×250mm ; 3.0×300mm ; | 316L Stainless Steel | Analytical |
4.0×20mm ; 4.0×30mm ; 4.0×50mm ; 4.0×100mm 4.0×150mm ; ; 4.0×200mm； 4.0×250mm ; 4.0×300mm | 316L Stainless Steel | Analytical |
4.6×20mm ; 4.6×30mm ; 4.6×50mm ; 4.6×100mm 4.6×150mm ; 4.6×200mm ; 4.6×250mm ; 4.6×300mm | 316L Stainless Steel | Analytical |
7.8×150mm ; 7.8×250mm ; 7.8×300mm | 316L Stainless Steel | Semi-prep |
10×50mm ; 10×100mm ; 10×150mm 10×200mm ; 10×250mm ; 10×300mm | 316L Stainless Steel | Semi-prep |
20×50mm ; 20×100mm ; 20×150mm ; 20×200mm 20×250mm ; 20×300mm | 316L Stainless Steel | Semi-prep |
21.2×150mm ; 21.2× 250mm | 316L Stainless Steel | Semi-prep |
