C18 HPLC Columns
ValuSil C18 Chromatography Column
ValuSil C18 is a cost-effective HPLC column. It uses a high-purity silica matrix bonded with an octadecyl (C18) stationary phase and is fully end-capped.
While meeting the USP L1 classification, it provides stable and reliable separation performance at an economical price—designed for routine analytical testing with consistent, traceable chromatographic data and pharmacopoeial-method compatibility.
It helps control procurement costs while meeting regulatory requirements, providing a balanced return on investment for QC, food testing, CRO and method-development labs.
ValuSil C18 HPLC columns
Features
Pick the ValuSil C18 for your needs—USP L1 performance with controlled cost.
- Compliance with USP L1 — Pharmacopoeial or registration methods developed with L1 columns can be transferred directly without redevelopment and revalidation.
- High-purity silica with strict metal-impurity control — Weakens secondary interactions, improves peak shape for basic samples, and reduces adsorption losses of polar components.
- Full end-capping — Improves peak symmetry for basic substances such as amines and alkaloids; reduces peak tailing for reliable resolution and quantitation.
- Good batch-to-batch reproducibility — Multi-batch testing shows retention-time RSD ≤ 1.5%, supporting high-throughput labs after column-batch changes.
Product Selection
Choose by particle size, then confirm the catalog SKU in the selection table. Additional specifications can be customized.
Specifications
Core stationary-phase and packing specifications for ValuSil C18.
| Parameter | Specification |
|---|---|
| Stationary Phase | Octadecyl-Bonded Silica (C18) |
| USP Classification | L1 |
| Particle Size | 1.8 μm, 3.0 μm, 5 μm |
| Pore Size | 120 Å |
| Specific Surface Area | 328 m²/g |
| Carbon Load | 16.4% |
| End-Capping | Fully End-Capped |
Product selection table
Ten standard catalog sizes. Additional specifications can be customized—contact our sales engineers.
| Product No. | Dimensions (mm) | Particle Size | Pore Size |
|---|---|---|---|
| P1F0B-00916 | 2.1×50 mm | 1.8 μm | 120 Å |
| P1F0B-00915 | 2.1×100 mm | 1.8 μm | 120 Å |
| P1F0B-00914 | 3.0×50 mm | 1.8 μm | 120 Å |
| P1F0B-00913 | 3.0×100 mm | 1.8 μm | 120 Å |
| P1F0B-00912 | 2.1×100 mm | 3 μm | 120 Å |
| P1F0B-00911 | 3.0×50 mm | 3 μm | 120 Å |
| P1F0B-00910 | 3.0×100 mm | 3 μm | 120 Å |
| P1F0B-00909 | 4.6×100 mm | 3 μm | 120 Å |
| P1F0B-00907 | 4.6×150 mm | 5 μm | 120 Å |
| P1F0B-00908 | 4.6×250 mm | 5 μm | 120 Å |
Applications
Cost-effective USP L1 performance for pharmaceutical QC, food compliance, CRO throughput and academic method work.
Pharmaceutical R&D and QC
Assay and related-substance testing of chemical-drug APIs and active ingredients in natural medicines. Supports GMP quality control and method transfer.
Food-safety monitoring
Preservatives, synthetic colorants, pesticide residues, vitamins and other food components—suited to complex matrices and compliance screening.
Third-party testing and CRO
Routine testing across pharmaceuticals, foods and environmental samples. Good batch reproducibility and a complete COA support audits.
Research institutes and universities
Conventional reversed-phase method exploration, reference-standard separation and high-volume screening with balanced performance and cost.
Application Cases
Results from product documentation using Hipsoul® (uHPLCs®) ValuSil C18, 5 μm, 120 Å, 4.6 × 250 mm.
Prednisone Acetate tablet
| No. | Name | Retention Time (min) | Peak Area (mAU·s) | Peak Height (mAU) | Plate Count | Resolution |
|---|---|---|---|---|---|---|
| 1 | Peak 1 | 21.717 | 7.24972 | 2.02E-01 | 7768 | / |
| 2 | Peak 2 | 26.277 | 7.11835 | 1.66E-01 | 7588 | 4.15 |
| 3 | Peak 3 | 30.633 | 1.97718 | 1.59E-01 | 146561 | 5.7 |
| 4 | Peak 4 Prednisone | 33.662 | 3402.79492 | 559.87433 | 713823 | 12.61 |
| 5 | Peak 5 | 33.929 | 17.23109 | 2.6219 | 627256 | 1.61 |
| 6 | Peak 6 | 35.565 | 4.52E-01 | 8.99E-02 | 1072487 | 10.58 |
Test results: resolution between all peaks was greater than 1.5, meeting the testing requirements.
Water-soluble vitamin mixed standard
| Peak Name | Retention Time (min) | Peak Area | Resolution (USP) | Tailing Factor | Plate Count (USP) |
|---|---|---|---|---|---|
| Vitamin C | 2.709 | 4576.52539 | / | 1.17980 | 3251 |
| Vitamin B9 | 3.397 | 2375.95850 | 3.93 | 1.37500 | 7265 |
| Vitamin B6 | 3.937 | 1249.22327 | 3.63 | 1.10169 | 13116 |
| Vitamin B5 | 4.649 | 3788.57617 | 4.57 | 1.08932 | 11503 |
| Vitamin B12 | 16.019 | 1094.09216 | 68.49 | 1.12805 | 164052 |
| Vitamin B2 | 17.477 | 306.35977 | 3.45 | 1.03030 | 157527 |
Test results: under these chromatographic conditions, the resolution of all vitamins met the testing requirements.
Support
Technical support for method comparison, troubleshooting and quality documentation.
Complimentary method consultation
Guidance on method comparison and conversion between ValuSil C18 and leading-brand C18 columns.
Rapid technical response
Professional technical support available 24/7, with troubleshooting solutions provided within 48 hours.
Quality consistency assurance
Each batch undergoes quality control for consistent performance parameters and is supplied with a test report.
FAQ
Answers based on product specifications and support commitments.
01 Is ValuSil C18 USP L1 compliant?
Yes. The ValuSil C18 bonded phase meets the USP L1 classification for octadecylsilane-bonded silica, a reversed-phase stationary phase widely used in pharmacopoeias and drug standards.
02 What particle sizes are available?
ValuSil C18 is offered in 1.8 μm, 3.0 μm and 5 μm particle sizes, all with 120 Å pore size. Additional specifications can be customized—contact our sales engineers.
03 How reproducible is batch-to-batch retention?
Testing across multiple batches shows a retention-time RSD of ≤ 1.5%, with low column-to-column and batch-to-batch variation.
04 Is the column fully end-capped?
Yes. Thorough end-capping improves peak symmetry for basic substances such as amines and alkaloids and reduces peak tailing.
05 What applications is ValuSil C18 suited for?
Typical uses include pharmaceutical R&D and QC, food-safety monitoring, third-party testing and CRO laboratories, and method development in research institutes and universities.
06 What support do you provide after purchase?
Complimentary method consultation, technical support with troubleshooting solutions within 48 hours, and a test report supplied with each column batch.
Need help matching your method?
Request a ValuSil C18 Quote
Share your current C18 brand, dimensions, particle size and quantity — we’ll match a ValuSil size and reply with availability and pricing.
Helpful to include:
- Current brand / dimensions
- Particle size (1.8 / 3 / 5 μm)
- Application or USP method
- Quantity / destination
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