How Does HPLC Work ？
How Does HPLC Work ？ High-Performance Liquid Chromatography, commonly known as HPLC, is a powerful
Guard Column in HPLC
Guard Column in HPLC OEM Manufacturer
uHPLCs is a professional OEM factory producing high-performance liquid chromatography (HPLC) guard columns. These guard columns protect the main column in an HPLC system, prolonging its lifespan and ensuring accurate and reliable results.
uHPLCs’s guard columns are manufactured using the highest quality stainless steel materials and are available in various sizes and specifications to meet the needs of different applications. With our experienced engineers and technicians, uHPLCs can provide guard column custom solution for customers, ensuring that the guard columns meet their specific requirements.
Furthermore, uHPLCs’s guard columns are competitively available, making them an affordable solution for laboratories and research institutions. If you require a high-quality guard column for your HPLC system, uHPLCs is the ideal choice.
① Analytical Guard Column
UHPLC Cartridge Size Option:
5μ, 3μ, 2μ, 1.8μ
Peek Cartridge Size Option:
5μ, 3μ, 2μ, 1.8
Peek Cartridge Size Option:
5μ
② Preparative Guard Column
Peek Cartridge Size Option:
10μ, 5μ
Clients Contact Us As Excellent Service
Expertise You Can Count On – Contact Us Now for Professional Assistance with HPLC Guard Columns
Professional HPLC R&D Team
Welcome to our Professional HPLC Guard Columns Factory Team. We pride ourselves on being experts in HPLC guard columns, and our team is dedicated to providing the highest quality products and services to our customers. With years of experience and a commitment to excellence, we are the go-to choice for anyone needing top-notch HPLC guard columns. Whether you need a custom solution or a standard product, our team is here to help.
Contact us today to learn more about how we can assist you with all your HPLC guard column needs.
1.High Quality
Our HPLC guard columns are made with the highest quality SUS316L and are manufactured to strict industry standards to ensure optimal performance and longevity.
2. Customizable
We offer a wide range of standard HPLC guard columns, but we also specialize in custom solutions that are tailored to meet the specific needs of your application.
3. Competitive Pricing
We offer competitive pricing on all our guard columns in HPLC system, so you can be sure that you are getting the best value for your money.
4. Experienced R&D Team
Our team of experts has extensive knowledge and experience in the field of guard columns, and we are dedicated to providing the best possible service to customers.
Comprehensive Support: We provide comprehensive support to our customers, including installation and troubleshooting assistance, to ensure that you get the most out of your HPLC guard columns.
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How Custom Your HPLC Guard Columns
Our HPLC guard columns can be customized to meet your application’s specific needs. Some ways in which we can customize our HPLC guard columns include the following:
- Material: Our HPLC guard columns can be made from various materials, such as stainless steel, PEEK, and fused silica, to suit the requirements of your application.
- Length: The length of the column can be customized to fit the specific dimensions of your instrument and to optimize the separation of your sample.
- Inner diameter (ID): The inner diameter of the column can be customized to match the ID of your analytical column, ensuring a smooth and efficient transfer of the sample.
- Pore size: The pore size of the guard column can be customized to match the size of your sample, ensuring that any contaminants are effectively removed.
- Phase: We can customize the guard column’s phase to match your analytical column’s phase. Just this will helps to improve the separation efficiency of your sample.
- End fittings: The end fittings of the guard column can be customized to match the connection type of your analytical column, ensuring a leak-free and secure connection.
- Packing: We can customize the packing material of the guard column to match your sample type and the separation mode you are using.
* Please note that the customization may depend on the materials and technology available in our factory. We will be happy to discuss your specific requirements and see how we can best meet your needs.
- Please note that the customization may depend on the materials and technology available in our factory. We will be happy to discuss your specific requirements and see how we can best meet your guard column needs.
High Quality HPLC Column 316L, 316 Stainless Steel Tube Materials
Design New HPLC Columns 100% As Clients Request
Make HPLC Column Samples And Test Before Mass Products.
What Clients Say
I've been using the HPLC guard columns from this factory for over a year now and I couldn't be happier with the performance. They are extremely reliable and have greatly improved the efficiency of my separations.
Amanda LeeCEO 
I recently switched to the custom HPLC guard columns from this factory and the difference in my results has been astounding. The team was extremely helpful in finding the perfect solution for my specific needs.
Adam CheiseDesigner 
I've been using HPLC guard columns from this factory for a number of years and I've never had a problem. They are top quality and the customer service is always excellent.
Floyd StallerCo-Founder 
I highly recommend the HPLC guard columns from this factory. They are made with high-quality materials and are extremely durable. Plus, the team is always available to help with any questions or concerns.
Floyd StallerCo-Founder
I was having trouble with contaminants in my samples until I started using the HPLC guard columns from this factory. They've made a huge difference in my results and I've been able to achieve much better separations.
Floyd StallerCo-Founder
I've tried different brands of HPLC guard columns in the past, but none have compared to the quality and performance of the ones from this factory. I highly recommend them to anyone in need of reliable and effective guard columns.
Floyd StallerCo-Founder
I've been extremely impressed with the HPLC guard columns from this factory. Not only are they customizable to my specific needs, but they have greatly improved the efficiency and accuracy of my separations.
Floyd StallerCo-Founder
I have been very pleased with the HPLC guard columns from this factory. They are of high quality, and their customer service is excellent. They have helped me to achieve better separations and save costs. I would recommend them to anyone.
Floyd StallerCo-Founder
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High Quality HPLC Column 316L, 316 Stainless Steel Tube Materials
Design New HPLC Columns 100% As Clients Request
Make HPLC Column Samples And Test Before Mass Products.
HPLC Guard Columns FAQ
What is HPLC Guard Column and Why Use Guard Column in HPLC System ?
In short, In High-Performance Liquid Chromatography (HPLC), a guard column is a small, short, and narrow-bore column that is positioned in front of the main analytical column. Its primary purpose is to protect the analytical column from sample matrix components, contaminants, and particulates that may adversely affect column performance and lead to premature column degradation.
Why You Should to Use a Guard Column in HPLC System?
Column Protection:
The guard column acts as a sacrificial column, intercepting impurities and particulates present in the sample or mobile phase. By doing so, it prevents these contaminants from reaching the main analytical column, which extends the lifespan and maintains the performance of the expensive analytical column.
Cost Savings:
Replacing the guard column is more economical than replacing the entire analytical column. Using a guard column helps minimize maintenance costs and extends the overall lifetime of the HPLC system.
Improved Column Efficiency:
By removing unwanted sample components, the guard column ensures that only the desired analytes enter the analytical column. This results in improved peak shapes and separation efficiency, leading to more accurate and reliable results.
Reduced Downtime:
The guard column helps to protect the analytical column from blockages and clogging. As a result, it reduces the frequency of maintenance and column replacements, leading to less downtime for the HPLC system.
Enhanced Method Robustness:
The presence of a guard column can increase the robustness of the HPLC method by providing an extra layer of protection against variations in sample matrices, which may occur in routine analysis.
Compatibility with Various Samples:
Guard columns can be tailored with different sorbent materials and selectivity to suit specific sample types and applications, making them versatile for various analyses.
In summary, using a guard column in an HPLC system is a prudent and cost-effective strategy to safeguard the analytical column, improve separation efficiency, and enhance the overall longevity and performance of the HPLC system.
HPLC Guard Columns Applications
Sample Pre-treatment: HPLC guard columns can be used as a pre-treatment step before the sample is injected into the analytical column, to remove any impurities or contaminants that may affect the accuracy of the analysis.
Reversed-Phase Chromatography: HPLC guard columns can be used in reversed-phase chromatography to remove any ionizable compounds that may interfere with the separation of the sample.
Normal-Phase Chromatography: HPLC guard columns can be used in normal-phase chromatography to remove any polar compounds that may interfere with the separation of the sample.
Gradient Elution: HPLC guard columns can be used in gradient elution to remove any compounds that may have low retention time and interfere with the separation of the sample.
Size Exclusion Chromatography: HPLC guard columns can be used in size exclusion chromatography to remove any large molecules that may interfere with the separation of the sample.
Ion Exchange Chromatography: HPLC guard columns can be used in ion exchange chromatography to remove any ions that may interfere with the separation of the sample.
Affinity Chromatography: HPLC guard columns can be used in affinity chromatography to remove any non-specific binding proteins that may interfere with the separation of the sample.
Multimodal Chromatography: HPLC guard columns can be used in multimodal chromatography to remove any compounds that may interfere with the separation of the sample by multiple mechanisms.
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Frequently Asked Questions
HPLC guard columns are small, packed columns that are placed in front of the analytical column in a HPLC system. They are used to remove impurities or contaminants that may affect the accuracy of the analysis and extend the life of the analytical column.
HPLC guard columns can be made of various materials such as stainless steel, PEEK, and fused silica. The material used will depend on the specific requirements of the application.
It depends on the type of sample you are analyzing, the frequency of use and the quality of the guard column. It is recommended to replace the guard column after 50-100 injections or when you notice a decrease in the performance of the column.
Yes, HPLC guard columns can be customized to fit the specific needs of your application, such as the length, inner diameter, pore size, phase, end fittings and packing material.
HPLC guard columns remove impurities or contaminants that may affect the accuracy of the analysis by trapping them before they reach the analytical column. The results in a cleaner and more accurate separation of the sample.
Yes, HPLC guard columns can be used with any type of HPLC system, including reverse phase, normal phase, size exclusion, ion exchange, affinity, and multimodal chromatography.
Installing an HPLC guard column is relatively simple. It is connected to the analytical column with the same fittings and placed before the analytical column in the mobile phase flow path.
The most common problem with HPLC guard columns is poor performance or clogging. To troubleshoot, check if the column is properly connected, check the mobile phase flow rate, and check if the guard column needs to be replaced. If the problem persists, it is recommended to contact the manufacturer for further assistance.
Need Help ?
Contact uHPLCs Today for Any Questions for HPLC / UHPLC
+(86) 0755-28502380
sales@uhplcs.com
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