Guard Column in HPLC

HPLC guard columns are small, packed columns that are placed in front of the analytical column in a HPLC system. They are used to remove impurities or contaminants that may affect the accuracy of the analysis and extend the life of the analytical column.

HPLC guard columns can be made of various materials such as stainless steel, PEEK, and fused silica. The material used will depend on the specific requirements of the application.

It depends on the type of sample you are analyzing, the frequency of use and the quality of the guard column. It is recommended to replace the guard column after 50-100 injections or when you notice a decrease in the performance of the column.

Yes, HPLC guard columns can be customized to fit the specific needs of your application, such as the length, inner diameter, pore size, phase, end fittings and packing material.

HPLC guard columns remove impurities or contaminants that may affect the accuracy of the analysis by trapping them before they reach the analytical column. The results in a cleaner and more accurate separation of the sample.

Yes, HPLC guard columns can be used with any type of HPLC system, including reverse phase, normal phase, size exclusion, ion exchange, affinity, and multimodal chromatography.

Installing an HPLC guard column is relatively simple. It is connected to the analytical column with the same fittings and placed before the analytical column in the mobile phase flow path.

The most common problem with HPLC guard columns is poor performance or clogging. To troubleshoot, check if the column is properly connected, check the mobile phase flow rate, and check if the guard column needs to be replaced. If the problem persists, it is recommended to contact the manufacturer for further assistance.