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How Does HPLC Work ? High-Performance Liquid Chromatography, commonly known as HPLC, is a powerful
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uHPLCs is a professional OEM factory producing high-performance liquid chromatography (HPLC) guard columns. These guard columns protect the main column in an HPLC system, prolonging its lifespan and ensuring accurate and reliable results.
uHPLCs’s guard columns are manufactured using the highest quality stainless steel materials and are available in various sizes and specifications to meet the needs of different applications. With our experienced engineers and technicians, uHPLCs can provide guard column custom solution for customers, ensuring that the guard columns meet their specific requirements.
Furthermore, uHPLCs’s guard columns are competitively available, making them an affordable solution for laboratories and research institutions. If you require a high-quality guard column for your HPLC system, uHPLCs is the ideal choice.
UHPLC Cartridge Size Option:
5μ, 3μ, 2μ, 1.8μ
Peek Cartridge Size Option:
5μ, 3μ, 2μ, 1.8
Peek Cartridge Size Option:
5μ
Peek Cartridge Size Option:
10μ, 5μ
Expertise You Can Count On – Contact Us Now for Professional Assistance with HPLC Guard Columns
Welcome to our Professional HPLC Guard Columns Factory Team. We pride ourselves on being experts in HPLC guard columns, and our team is dedicated to providing the highest quality products and services to our customers. With years of experience and a commitment to excellence, we are the go-to choice for anyone needing top-notch HPLC guard columns. Whether you need a custom solution or a standard product, our team is here to help.
Contact us today to learn more about how we can assist you with all your HPLC guard column needs.
Our HPLC guard columns are made with the highest quality SUS316L and are manufactured to strict industry standards to ensure optimal performance and longevity.
We offer a wide range of standard HPLC guard columns, but we also specialize in custom solutions that are tailored to meet the specific needs of your application.
We offer competitive pricing on all our guard columns in HPLC system, so you can be sure that you are getting the best value for your money.
Our team of experts has extensive knowledge and experience in the field of guard columns, and we are dedicated to providing the best possible service to customers.
Comprehensive Support: We provide comprehensive support to our customers, including installation and troubleshooting assistance, to ensure that you get the most out of your HPLC guard columns.
Our HPLC guard columns can be customized to meet your application’s specific needs. Some ways in which we can customize our HPLC guard columns include the following:
* Please note that the customization may depend on the materials and technology available in our factory. We will be happy to discuss your specific requirements and see how we can best meet your needs.
High Quality HPLC Column 316L, 316 Stainless Steel Tube Materials
Design New HPLC Columns 100% As Clients Request
Make HPLC Column Samples And Test Before Mass Products.
High Quality HPLC Column 316L, 316 Stainless Steel Tube Materials
Design New HPLC Columns 100% As Clients Request
Make HPLC Column Samples And Test Before Mass Products.
In short, In High-Performance Liquid Chromatography (HPLC), a guard column is a small, short, and narrow-bore column that is positioned in front of the main analytical column. Its primary purpose is to protect the analytical column from sample matrix components, contaminants, and particulates that may adversely affect column performance and lead to premature column degradation.
The guard column acts as a sacrificial column, intercepting impurities and particulates present in the sample or mobile phase. By doing so, it prevents these contaminants from reaching the main analytical column, which extends the lifespan and maintains the performance of the expensive analytical column.
Replacing the guard column is more economical than replacing the entire analytical column. Using a guard column helps minimize maintenance costs and extends the overall lifetime of the HPLC system.
By removing unwanted sample components, the guard column ensures that only the desired analytes enter the analytical column. This results in improved peak shapes and separation efficiency, leading to more accurate and reliable results.
The guard column helps to protect the analytical column from blockages and clogging. As a result, it reduces the frequency of maintenance and column replacements, leading to less downtime for the HPLC system.
The presence of a guard column can increase the robustness of the HPLC method by providing an extra layer of protection against variations in sample matrices, which may occur in routine analysis.
Guard columns can be tailored with different sorbent materials and selectivity to suit specific sample types and applications, making them versatile for various analyses.
In summary, using a guard column in an HPLC system is a prudent and cost-effective strategy to safeguard the analytical column, improve separation efficiency, and enhance the overall longevity and performance of the HPLC system.
Sample Pre-treatment: HPLC guard columns can be used as a pre-treatment step before the sample is injected into the analytical column, to remove any impurities or contaminants that may affect the accuracy of the analysis.
Reversed-Phase Chromatography: HPLC guard columns can be used in reversed-phase chromatography to remove any ionizable compounds that may interfere with the separation of the sample.
Normal-Phase Chromatography: HPLC guard columns can be used in normal-phase chromatography to remove any polar compounds that may interfere with the separation of the sample.
Gradient Elution: HPLC guard columns can be used in gradient elution to remove any compounds that may have low retention time and interfere with the separation of the sample.
Size Exclusion Chromatography: HPLC guard columns can be used in size exclusion chromatography to remove any large molecules that may interfere with the separation of the sample.
Ion Exchange Chromatography: HPLC guard columns can be used in ion exchange chromatography to remove any ions that may interfere with the separation of the sample.
Affinity Chromatography: HPLC guard columns can be used in affinity chromatography to remove any non-specific binding proteins that may interfere with the separation of the sample.
Multimodal Chromatography: HPLC guard columns can be used in multimodal chromatography to remove any compounds that may interfere with the separation of the sample by multiple mechanisms.
HPLC guard columns are small, packed columns that are placed in front of the analytical column in a HPLC system. They are used to remove impurities or contaminants that may affect the accuracy of the analysis and extend the life of the analytical column.
HPLC guard columns can be made of various materials such as stainless steel, PEEK, and fused silica. The material used will depend on the specific requirements of the application.
It depends on the type of sample you are analyzing, the frequency of use and the quality of the guard column. It is recommended to replace the guard column after 50-100 injections or when you notice a decrease in the performance of the column.
Yes, HPLC guard columns can be customized to fit the specific needs of your application, such as the length, inner diameter, pore size, phase, end fittings and packing material.
HPLC guard columns remove impurities or contaminants that may affect the accuracy of the analysis by trapping them before they reach the analytical column. The results in a cleaner and more accurate separation of the sample.
Yes, HPLC guard columns can be used with any type of HPLC system, including reverse phase, normal phase, size exclusion, ion exchange, affinity, and multimodal chromatography.
Installing an HPLC guard column is relatively simple. It is connected to the analytical column with the same fittings and placed before the analytical column in the mobile phase flow path.
The most common problem with HPLC guard columns is poor performance or clogging. To troubleshoot, check if the column is properly connected, check the mobile phase flow rate, and check if the guard column needs to be replaced. If the problem persists, it is recommended to contact the manufacturer for further assistance.
Contact uHPLCs Today for Any Questions for HPLC / UHPLC
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