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HPLC Applications

Popular HPLC Application Industry

Here, We Supply Over 30 Kinds Of HPLC Column and Accessories , Fitting Application For Your Scientific Analysis of Material Composition

Pharmaceutical Industry

High-performance liquid chromatography (HPLC) is a widely used technique in the pharmaceutical industry for the analysis and characterization of drugs and drug products. HPLC can be used to separate, identify, and quantify the components of a sample, including small molecules, peptides, proteins, and nucleic acids.

Medicine Manufacture

High-performance liquid chromatography (HPLC) is a widely used analytical technique in medicine manufacture for quality control and testing of active pharmaceutical ingredients (APIs), excipients, drug products, and packaging materials


Nitrosamines are a group of organic compounds that are classified as probable human carcinogens. They can be found in a variety of products, including food, drugs, and consumer products, as a result of chemical reactions that occur during manufacturing or processing.

HPLC Analytics & Research
in Academic

High-performance liquid chromatography (HPLC) is a powerful analytical tool used in academic research for the separation, identification, and quantification of complex mixtures. Here are some common applications of HPLC in academic research

HPLC Analytic for
Horticultural Research

HPLC is an important analytical tool in horticultural research, as it allows for the identification and quantification of plant compounds. HPLC is used to measure the levels of phytochemicals, such as flavonoids and carotenoids, which are important for plant growth, health, and disease resistance.

HPLC Applications in Biopharmaceutical Industry

HPLC is a key analytical technique in the biopharmaceutical industry, where it is used to analyze the purity and potency of biologic drugs, such as antibodies and recombinant proteins. HPLC is also used to detect impurities and degradation products that can affect the safety and efficacy of biologic drugs.

HPLC Application for
Chemicals and Energy

HPLC is used in the chemical and energy industries to analyze the purity and quality of chemicals, such as polymers, fuels, and lubricants. HPLC can also be used to detect and quantify trace impurities that can affect the performance and safety of these chemicals.

HPLC Application for
Cannabis and Hemp

HPLC is a valuable analytical tool in the cannabis and hemp industries, where it is used to measure the levels of cannabinoids, such as THC and CBD, in plant material and products. HPLC is also used to detect contaminants, such as pesticides and heavy metals, that can affect the quality and safety of cannabis and hemp products.

HPLC Applications in
Clinical Research

High Performance Liquid Chromatography (HPLC) has numerous applications in clinical research. It is used for the analysis of biomolecules such as drugs, proteins, and peptides in biological samples. HPLC is widely used in the pharmaceutical industry for drug development and analysis, and in clinical laboratories for the diagnosis and monitoring of diseases.

Environmental Analysis

HPLC is a powerful analytical technique for environmental analysis. It is used to detect and quantify organic compounds in soil, water, and air samples. HPLC can separate and identify complex mixtures of chemicals, making it an essential tool for environmental monitoring and pollution control.

Food and Beverage

HPLC is widely used in the food and beverage industry for quality control and product development. It is used to analyze food additives, preservatives, and contaminants, as well as to quantify nutrients such as vitamins and amino acids. HPLC can also be used to detect and quantify food allergens.

Forensic Toxicology

HPLC is a key analytical technique in forensic toxicology. It is used to identify and quantify drugs, poisons, and metabolites in biological samples such as blood, urine, and hair. HPLC is also used to detect and quantify alcohol and other volatile compounds in breath and blood samples for legal purposes.

PFAS HPLC Application

Per- and polyfluoroalkyl substances (PFAS) are a group of chemicals that are widely used in industrial and consumer products. HPLC is a powerful analytical tool for the detection and quantification of PFAS in environmental and biological samples. HPLC can separate and identify different types of PFAS, which is important for understanding their sources and potential health effects.

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FAQ for HPLC Application

Some Popular Questions About HPLC Applications

HPLC stands for High Performance Liquid Chromatography. It is an analytical technique used to separate, identify, and quantify components in a mixture. It works by passing a liquid sample through a column packed with a stationary phase, which separates the components based on their physicochemical properties.

There are several types of HPLC detectors, including UV-Vis, fluorescence, electrochemical, mass spectrometry, and refractive index detectors. Each detector has its advantages and limitations, depending on the type of sample being analyzed.

UPLC stands for Ultra Performance Liquid Chromatography, which is a more advanced and efficient version of HPLC. UPLC uses smaller particles in the stationary phase, resulting in faster separation times and higher resolution.

The key parameters to consider when developing an HPLC method include the mobile phase composition, column type, flow rate, temperature, and detector type.

HPLC has a wide range of applications, including drug development and analysis, clinical research, environmental analysis, food and beverage analysis, forensic toxicology, and more.

There are several types of HPLC columns, including reverse phase, normal phase, ion exchange, size exclusion, and affinity columns.

A packed column is pre-filled with the stationary phase material, while an empty column requires the user to pack the stationary phase material into the column before use.

The frequency of HPLC column replacement depends on several factors, including the type of column, sample matrix, and column usage. In general, columns should be replaced when they begin to show a decrease in separation efficiency or when they reach the end of their useful life.


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