Preparative HPLC Columns
USHD Prep HPLC Columns
These columns are for collecting a compound, not only measuring it. The selection sheet lists USHD C18, C18-AQ, C18-AR, C18-T, and C8, all at 120 Å.
Internal diameter splits the list. 10×150 mm and 10×250 mm are semi-preparative. 20 mm, 21.2 mm, 30 mm, and 50 mm are preparative. Lengths on the sheet are 150 mm and 250 mm.
Semi-preparative column hardware
Column Overview
The video already on this page.
Features
One catalog, two scales. The split follows the internal diameter on the selection sheet.
- Semi-preparative — 10×150 mm and 10×250 mm. Higher load than a 4.6 mm analytical column, and still in the range many analytical systems can run.
- Preparative — 20, 21.2, 30, and 50 mm ID, at 150 or 250 mm. These are the sizes for fraction collection. 21.2 mm is on the sheet for C18, C18-AR, and C18-T, not for C18-AQ or C8.
- Loading, not just a chromatogram — The current page describes these columns for isolating APIs, peptides, natural products, and fine chemicals, with stainless steel hardware and high-purity silica.
- Pore size on this sheet — Every listed size is 120 Å. Particle size is 10 μm or 5 μm, and USHD C18 also has two 8 μm sizes.
Product Selection
Choose the scale first, then the phase. 20 sizes are 10 mm. 72 sizes are 20 mm or larger.
Semi-preparative
10×150 and 10×250 mm
All five phases at 5 μm and 10 μm. No 8 μm size at 10 mm ID.
View C18 →
Preparative
20, 21.2, 30, and 50 mm ID
150 mm and 250 mm lengths. 21.2 mm is listed for C18, C18-AR, and C18-T.
View C18-AR →
USHD C18
10 μm, 8 μm, and 5 μm. The only 8 μm rows are 30×250 and 50×250 mm.
View catalog →
USHD C18-AQ
10 μm and 5 μm. No 21.2 mm size on this sheet.
View catalog →
USHD C18-AR
10 μm and 5 μm, including 21.2 mm.
View catalog →
USHD C18-T
10 μm and 5 μm, including 21.2 mm. Four 10 μm numbers are also printed on C18-AR.
View catalog →
USHD C8
10 μm and 5 μm. No 21.2 mm and no 8 μm on this sheet.
View catalog →
Specifications
Every catalog row below is 120 Å. Scale is taken from the internal diameter: 10 mm is semi-preparative, and 20 mm or larger is preparative.
| Phase | Particle sizes on the sheet | Pore size | Semi-prep | Prep |
|---|---|---|---|---|
| USHD C18 | 10 μm, 8 μm, 5 μm | 120 Å | 4 | 16 |
| USHD C18-AQ | 10 μm, 5 μm | 120 Å | 4 | 12 |
| USHD C18-AR | 10 μm, 5 μm | 120 Å | 4 | 16 |
| USHD C18-T | 10 μm, 5 μm | 120 Å | 4 | 16 |
| USHD C8 | 10 μm, 5 μm | 120 Å | 4 | 12 |
USHD C18
4 semi-preparative sizes at 10 mm ID. 16 preparative sizes at 20 mm ID or larger. All 120 Å.
Semi-preparative, 10 mm
| Catalog No. | Dimension | Particle size | Pore size | Scale |
|---|---|---|---|---|
| P1F0B-00577 | 10×150 mm | 10 μm | 120 Å | Semi-prep |
| P1F0B-00578 | 10×250 mm | 10 μm | 120 Å | Semi-prep |
| P1F0B-00587 | 10×150 mm | 5 μm | 120 Å | Semi-prep |
| P1F0B-00572 | 10×250 mm | 5 μm | 120 Å | Semi-prep |
Preparative, 20 mm and larger
| Catalog No. | Dimension | Particle size | Pore size | Scale |
|---|---|---|---|---|
| P1F0B-00579 | 20×150 mm | 10 μm | 120 Å | Prep |
| P1F0B-00580 | 20×250 mm | 10 μm | 120 Å | Prep |
| P1F0B-00581 | 30×150 mm | 10 μm | 120 Å | Prep |
| P1F0B-00582 | 30×250 mm | 10 μm | 120 Å | Prep |
| P1F0B-00585 | 50×150 mm | 10 μm | 120 Å | Prep |
| P1F0B-00586 | 50×250 mm | 10 μm | 120 Å | Prep |
| P1F0B-00831 | 30×250 mm | 8 μm | 120 Å | Prep |
| P1F0B-00832 | 50×250 mm | 8 μm | 120 Å | Prep |
| P1F0B-00589 | 20×150 mm | 5 μm | 120 Å | Prep |
| P1F0B-00590 | 20×250 mm | 5 μm | 120 Å | Prep |
| P1F0B-00833 | 21.2×150 mm | 5 μm | 120 Å | Prep |
| P1F0B-00834 | 21.2×250 mm | 5 μm | 120 Å | Prep |
| P1F0B-00591 | 30×150 mm | 5 μm | 120 Å | Prep |
| P1F0B-00592 | 30×250 mm | 5 μm | 120 Å | Prep |
| P1F0B-00595 | 50×150 mm | 5 μm | 120 Å | Prep |
| P1F0B-00596 | 50×250 mm | 5 μm | 120 Å | Prep |
USHD C18-AQ
4 semi-preparative sizes at 10 mm ID. 12 preparative sizes at 20 mm ID or larger. All 120 Å.
Semi-preparative, 10 mm
| Catalog No. | Dimension | Particle size | Pore size | Scale |
|---|---|---|---|---|
| P1F0B-00597 | 10×150 mm | 10 μm | 120 Å | Semi-prep |
| P1F0B-00598 | 10×250 mm | 10 μm | 120 Å | Semi-prep |
| P1F0B-00607 | 10×150 mm | 5 μm | 120 Å | Semi-prep |
| P1F0B-00569 | 10×250 mm | 5 μm | 120 Å | Semi-prep |
Preparative, 20 mm and larger
| Catalog No. | Dimension | Particle size | Pore size | Scale |
|---|---|---|---|---|
| P1F0B-00574 | 20×150 mm | 10 μm | 120 Å | Prep |
| P1F0B-00573 | 20×250 mm | 10 μm | 120 Å | Prep |
| P1F0B-00601 | 30×150 mm | 10 μm | 120 Å | Prep |
| P1F0B-00602 | 30×250 mm | 10 μm | 120 Å | Prep |
| P1F0B-00603 | 50×150 mm | 10 μm | 120 Å | Prep |
| P1F0B-00606 | 50×250 mm | 10 μm | 120 Å | Prep |
| P1F0B-00609 | 20×150 mm | 5 μm | 120 Å | Prep |
| P1F0B-00610 | 20×250 mm | 5 μm | 120 Å | Prep |
| P1F0B-00611 | 30×150 mm | 5 μm | 120 Å | Prep |
| P1F0B-00612 | 30×250 mm | 5 μm | 120 Å | Prep |
| P1F0B-00615 | 50×150 mm | 5 μm | 120 Å | Prep |
| P1F0B-00616 | 50×250 mm | 5 μm | 120 Å | Prep |
USHD C18-AR
4 semi-preparative sizes at 10 mm ID. 16 preparative sizes at 20 mm ID or larger. All 120 Å.
Semi-preparative, 10 mm
| Catalog No. | Dimension | Particle size | Pore size | Scale |
|---|---|---|---|---|
| P1F0B-00835 | 10×150 mm | 10 μm | 120 Å | Semi-prep |
| P1F0B-00836 | 10×250 mm | 10 μm | 120 Å | Semi-prep |
| P1F0B-00769 | 10×150 mm | 5 μm | 120 Å | Semi-prep |
| P1F0B-00770 | 10×250 mm | 5 μm | 120 Å | Semi-prep |
Preparative, 20 mm and larger
| Catalog No. | Dimension | Particle size | Pore size | Scale |
|---|---|---|---|---|
| P1F0B-00837 | 20×150 mm | 10 μm | 120 Å | Prep |
| P1F0B-00838 | 20×250 mm | 10 μm | 120 Å | Prep |
| P1F0B-00839 | 21.2×150 mm | 10 μm | 120 Å | Prep |
| P1F0B-00840 | 21.2×250 mm | 10 μm | 120 Å | Prep |
| P1F0B-00841 | 30×150 mm | 10 μm | 120 Å | Prep |
| P1F0B-00842 | 30×250 mm | 10 μm | 120 Å | Prep |
| P1F0B-00843 | 50×150 mm | 10 μm | 120 Å | Prep |
| P1F0B-00844 | 50×250 mm | 10 μm | 120 Å | Prep |
| P1F0B-00845 | 20×150 mm | 5 μm | 120 Å | Prep |
| P1F0B-00846 | 20×250 mm | 5 μm | 120 Å | Prep |
| P1F0B-00847 | 21.2×150 mm | 5 μm | 120 Å | Prep |
| P1F0B-00848 | 21.2×250 mm | 5 μm | 120 Å | Prep |
| P1F0B-00849 | 30×150 mm | 5 μm | 120 Å | Prep |
| P1F0B-00850 | 30×250 mm | 5 μm | 120 Å | Prep |
| P1F0B-00851 | 50×150 mm | 5 μm | 120 Å | Prep |
| P1F0B-00852 | 50×250 mm | 5 μm | 120 Å | Prep |
USHD C18-T
4 semi-preparative sizes at 10 mm ID. 16 preparative sizes at 20 mm ID or larger. All 120 Å.
Semi-preparative, 10 mm
| Catalog No. | Dimension | Particle size | Pore size | Scale |
|---|---|---|---|---|
| P1F0B-00887 | 10×150 mm | 10 μm | 120 Å | Semi-prep |
| P1F0B-00888 | 10×250 mm | 10 μm | 120 Å | Semi-prep |
| P1F0B-00617 | 10×150 mm | 5 μm | 120 Å | Semi-prep |
| P1F0B-00618 | 10×250 mm | 5 μm | 120 Å | Semi-prep |
Preparative, 20 mm and larger
| Catalog No. | Dimension | Particle size | Pore size | Scale |
|---|---|---|---|---|
| P1F0B-00889 | 20×150 mm | 10 μm | 120 Å | Prep |
| P1F0B-00890 | 20×250 mm | 10 μm | 120 Å | Prep |
| P1F0B-00853 | 21.2×150 mm | 10 μm | 120 Å | Prep |
| P1F0B-00854 | 21.2×250 mm | 10 μm | 120 Å | Prep |
| P1F0B-00841 | 30×150 mm | 10 μm | 120 Å | Prep |
| P1F0B-00842 | 30×250 mm | 10 μm | 120 Å | Prep |
| P1F0B-00843 | 50×150 mm | 10 μm | 120 Å | Prep |
| P1F0B-00844 | 50×250 mm | 10 μm | 120 Å | Prep |
| P1F0B-00619 | 20×150 mm | 5 μm | 120 Å | Prep |
| P1F0B-00620 | 20×250 mm | 5 μm | 120 Å | Prep |
| P1F0B-00855 | 21.2×150 mm | 5 μm | 120 Å | Prep |
| P1F0B-00856 | 21.2×250 mm | 5 μm | 120 Å | Prep |
| P1F0B-00621 | 30×150 mm | 5 μm | 120 Å | Prep |
| P1F0B-00622 | 30×250 mm | 5 μm | 120 Å | Prep |
| P1F0B-00625 | 50×150 mm | 5 μm | 120 Å | Prep |
| P1F0B-00626 | 50×250 mm | 5 μm | 120 Å | Prep |
P1F0B-00841, P1F0B-00842, P1F0B-00843, and P1F0B-00844 are printed on this sheet for USHD C18-T, 10 μm, at 30×150, 30×250, 50×150, and 50×250 mm. The same four numbers are also printed for USHD C18-AR, 10 μm, at those dimensions. Confirm the phase before ordering those four sizes.
USHD C8
4 semi-preparative sizes at 10 mm ID. 12 preparative sizes at 20 mm ID or larger. All 120 Å.
Semi-preparative, 10 mm
| Catalog No. | Dimension | Particle size | Pore size | Scale |
|---|---|---|---|---|
| P1F0B-00857 | 10×150 mm | 10 μm | 120 Å | Semi-prep |
| P1F0B-00858 | 10×250 mm | 10 μm | 120 Å | Semi-prep |
| P1F0B-00794 | 10×150 mm | 5 μm | 120 Å | Semi-prep |
| P1F0B-00864 | 10×250 mm | 5 μm | 120 Å | Semi-prep |
Preparative, 20 mm and larger
| Catalog No. | Dimension | Particle size | Pore size | Scale |
|---|---|---|---|---|
| P1F0B-00859 | 20×150 mm | 10 μm | 120 Å | Prep |
| P1F0B-00798 | 20×250 mm | 10 μm | 120 Å | Prep |
| P1F0B-00860 | 30×150 mm | 10 μm | 120 Å | Prep |
| P1F0B-00861 | 30×250 mm | 10 μm | 120 Å | Prep |
| P1F0B-00862 | 50×150 mm | 10 μm | 120 Å | Prep |
| P1F0B-00863 | 50×250 mm | 10 μm | 120 Å | Prep |
| P1F0B-00795 | 20×150 mm | 5 μm | 120 Å | Prep |
| P1F0B-00799 | 20×250 mm | 5 μm | 120 Å | Prep |
| P1F0B-00865 | 30×150 mm | 5 μm | 120 Å | Prep |
| P1F0B-00866 | 30×250 mm | 5 μm | 120 Å | Prep |
| P1F0B-00867 | 50×150 mm | 5 μm | 120 Å | Prep |
| P1F0B-00868 | 50×250 mm | 5 μm | 120 Å | Prep |
Applications
Uses already described on this page: collection of a compound, not only a peak measurement.
APIs
Isolation of active pharmaceutical ingredients at a higher load than an analytical column.
Peptides and proteins
Named on the current page among the purification uses.
Natural products
Extract isolation, where the loading and the fraction size matter.
Fine chemicals
Intermediates and other compounds the page lists for production-scale collection.
Food and cosmetic ingredients
Purification of ingredients, as listed in the current applications section.
Method scale-up
Start on 10 mm when the analytical method is ready, then move to 20 mm or larger when the mass requires it.
Analytical versions of these phases: USHD C18 HPLC columns · USHD C8 HPLC columns · All HPLC columns.
Support
Send the phase, the scale, and the catalog number if it is in the table.
Scale
10 mm for semi-prep. 20, 21.2, 30, or 50 mm for prep. Say which HPLC system and flow you have if you are unsure.
Catalog confirmation
We match the P1F0B number to phase, particle size, and dimensions. The four repeated C18-AR / C18-T numbers are confirmed before quoting.
Custom columns
Other IDs up to the 300 mm range named on this page, other lengths, and phases such as PFP, Phenyl, HILIC, SEC, or normal phase can be requested.
FAQ
Answers from the current prep page and the preparative selection sheet.
01 Should these columns be split into semi-prep and prep?
Yes, by internal diameter. On this sheet, 10×150 mm and 10×250 mm are the semi-preparative sizes. 20 mm, 21.2 mm, 30 mm, and 50 mm internal diameters are preparative. The sheet title groups them all as preparative columns. The 10 mm size is the one that still fits many analytical HPLC systems at a higher flow. The larger IDs need a preparative pump and fraction collection.
02 What is a prep HPLC column?
A preparative column isolates a compound so it can be collected, rather than only measuring it. The current page describes larger internal diameters, higher sample loading, and higher flow than an analytical column. Uses named on the page are APIs, peptides and proteins, natural products, fine chemicals, and food or cosmetic ingredients.
03 What does the selection sheet actually list?
Five phases, all 120 Å: USHD C18, C18-AQ, C18-AR, C18-T, and C8. Particle sizes on the sheet are 10 μm and 5 μm, plus 8 μm for USHD C18 only. Lengths are 150 mm and 250 mm. Internal diameters are 10, 20, 21.2, 30, and 50 mm. 21.2 mm is listed for C18 at 5 μm, and for C18-AR and C18-T at 10 μm and 5 μm. The 8 μm C18 rows are only 30×250 mm and 50×250 mm.
04 What about the older 150 Å and sub-5 μm cards?
The previous product cards on this page listed 150 Å, 3.5 μm, 3 μm, and 1.8 μm, and they were labeled as UPLC columns. Those particles and the 150 Å pore size are not on this preparative sheet, so they are not in the catalog tables. If you need one of those, send it as a custom request.
05 Which catalog number is the 10×250 mm semi-prep C18 column?
USHD C18, 10 μm, 120 Å, 10×250 mm is P1F0B-00578. USHD C18, 5 μm, 120 Å, 10×250 mm is P1F0B-00572. The 10×150 mm pair is P1F0B-00577 at 10 μm and P1F0B-00587 at 5 μm.
06 What hardware and other phases does the page offer?
The page lists 316L stainless steel, PEEK, and glass-lined column bodies. It also names PFP, Phenyl, HILIC, SEC, and normal phase as available bonded phases, and a broader size range of 10–300 mm ID and 150–1000 mm length. Those are not on this sheet. Ask for them as a custom column.
07 Why do four catalog numbers appear twice?
P1F0B-00841 through P1F0B-00844 are printed twice on the sheet: once under USHD C18-AR, 10 μm, and once under USHD C18-T, 10 μm, for 30×150, 30×250, 50×150, and 50×250 mm. The page shows both rows and flags them so the phase can be confirmed.
08 Can other dimensions be made?
Yes. The current page says dimensions, bonded phase, particle size, and end fittings can be made to match the purification system. The tables are the standard set from this sheet.
Need a column sized for the mass you collect?
Request a USHD Prep Quote
Send the catalog number, or the phase, particle size, and whether you need 10 mm or a larger ID.
Helpful to include:
- Phase (C18, C18-AQ, C18-AR, C18-T, or C8)
- Semi-prep (10 mm) or prep (20 mm and larger)
- Catalog number, if it is in the table
- Quantity and destination
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