uHPLCs

Types of Columns Used in HPLC Introduction High-performance liquid chromatography (HPLC) is a technique that is used to separate and quantify the components of a mixture. It does this by using a column that is packed with a stationary phase. The stationary phase can be… Read More »How Many Types of Columns Used in HPLC ?

What is the solvent effect? The solvent effect is the influence of the solvent on the physical and chemical properties of a solute. Solvents can affect a wide range of properties, including solubility, reactivity, stability, and spectroscopy. There are a number of different mechanisms by… Read More »How to Solve the “Solvent Effect” in High-Performance Liquid Chromatography (HPLC)

I. Introduction High-Performance Liquid Chromatography (HPLC) is a powerful tool used in analytical chemistry to separate, identify, and quantify components in a mixture.  Despite its capabilities, HPLC columns can face a range of problems that can affect their performance and the accuracy of results.  Common… Read More »One Guard Column Can Solve Most of the HPLC Problems ？

Understanding Trailing Factor, Symmetry Factor, and Asymmetry Factor in Chromatography In chromatography experiments, three terms that are often encountered but not always well understood are trailing factor, symmetry factor, and asymmetry factor. This article aims to clarify the relationship and differences between these three parameters… Read More »Understanding Chromatography Peaks: A Closer Look at Trailing, Symmetry, and Asymmetry Factors

As we Know Guard Column is a important parts for HPLC Column and HPLC System.  High-Performance Liquid Chromatography (HPLC) is a staple technique in numerous labs across the globe, allowing researchers and analysts to separate, identify, and quantify compounds in complex mixtures. While the main… Read More »Top 5 Reasons Why Your Lab Should Use an HPLC Guard Column

What is HPLC? High-Performance Liquid Chromatography (HPLC) is a widely-used analytical technique in chemistry and biochemistry. It is employed to identify, quantify, and separate components in a mixture. HPLC utilizes a liquid solvent as the mobile phase to carry the sample mixture through a column… Read More »How to Calculate Dwell Volume in HPLC

1. Simple Introduction Brief Overview of HPLC and its Significance High-Performance Liquid Chromatography, commonly known as HPLC, is a widely utilized analytical technique in both research and industry. It serves as a powerful tool for the separation, identification, and quantification of various components in a… Read More »Everything About Chromatography Column Packing You Should Know

How Does HPLC Work ？ High-Performance Liquid Chromatography, commonly known as HPLC, is a powerful analytical technique used across various industries to separate, identify, and quantify components in a mixture. Its versatility and precision have made it an indispensable tool in laboratories worldwide. Brief Overview… Read More »How Does HPLC Work ？

What is a frit on a chromatography column? A frit in a chromatography column, particularly in High-Performance Liquid Chromatography (HPLC), refers to a porous disk or barrier. Typically made from sintered stainless steel or other materials such as polyethylene or PTFE, the frit acts as… Read More »What is a frit on a chromatography column?

1. What is HPLC Solvents HPLC Solvents, also known as High-Performance Liquid Chromatography Solvents, are a group of pure organic liquids used in High-Performance Liquid Chromatography (HPLC). HPLC is an analytical technique used to separate, identify, and quantify various compounds in a mixture. It is… Read More »All About HPLC Solvents You Should Know