uHPLCs

Dead Volume and its Role in HPLC Systems In high-performance liquid chromatography (HPLC), dead volume refers to the overall volume of the system that the mobile phase flows through but doesn’t contribute to the separation process. Think of it as the nooks and crannies –… Read More »The Dead Volume Advantage: Thriving Beyond Peak Constraints

 Sample pretreatment is the process of sample preparation and extraction, purification and concentration of the components to be measured in the sample.  Sample pretreatment aims to eliminate matrix interference, protect the instrument, and improve the assay’s sensitivity, selectivity, accuracy, and precision. Sample Pretreatment Method Sample… Read More »4 Tips for Sample Pretreatment in Liquid Chromatography

Mobile phase composition plays a crucial role in most separations. In most cases, mobile phase is mixed of water and organic solvents. In isocratic operation the composition is pre- decided and remains unchanged during the analytical run.  On the other hand in gradient elution the final… Read More »Key Strategies for Choosing the Right Mobile Phase in Chromatography

High-performance liquid chromatography (HPLC) stands as a cornerstone in the world of analytical chemistry, providing unparalleled precision and efficiency in separating complex mixtures.  The heart of HPLC’s efficacy lies not just in the sophisticated machinery but significantly in the column packing, a seemingly minute yet… Read More »How Does HPLC Column Packing Affect Column Performance

Preparative HPLC is often associated with large capacity columns and high flow rates. However, it is not the size of the equipment and the amount of mobile phase consumed by the system that determines preparative HPLC but rather the purpose of the separation. Unlike the… Read More »Main use and technical characteristics of the preparative liquid phase

As we know , Maintaining a clean mobile phase from start to finish is crucial for reliable HPLC analysis.  High-quality filters play a vital role in filtering out impurities before they reach the sensitive separation stage in the column, ensuring consistently robust methods and excellent… Read More »Filters and Filtration in Liquid Chromatography System

What Do You Mean for Ghost Peaks?  When doing the gradient elution of liquid chromatography reversed-phase system, sometimes we will encounter the situation that the instrument does not feed the sample but also has the peak, affecting the quantitative results.  It can also make the… Read More »3 Reasons for Ghost Peaks and Solutions

In liquid chromatography (LC), “volume” can have several different meanings depending on the context. Here are three main interpretations: 1. Void volume (Vm): This refers to the total volume of liquid (mobile phase) contained within the column. It essentially represents the space not occupied by… Read More »What is the meaning of “volume” in liquid chromatography

High-performance liquid chromatography is an important branch of chromatography. This method has become an important separation and analysis technique in chemistry, medicine, industry, agronomy, commodity inspection, and legal inspection. Taking the pharmaceutical field as an example: the Chinese Pharmacopoeia contains a comparison table of high-performance… Read More »30 HPLC Problems and Solutions You Should Know

Here we provides valuable insights and practical tips for developing analytical conditions in high-performance liquid chromatography (HPLC). Hope This will let you know details of Analysis Of Condition Filtering Methods and help you to find the right filter method for your project. 1. Understanding the target… Read More »Analysis of Condition Filtering Methods