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Introduction In High Performance Liquid Chromatography (HPLC), the purity of solvents is paramount for achieving reliable and accurate results. Solvent filtration is a critical step in preparing these solvents, as it ensures the removal of particulate matter, undissolved solids, and other contaminants that could adversely… Read More »Types of HPLC Solvent Filters and How to Choose

High-performance liquid chromatography (HPLC) is a workhorse technique in labs worldwide, separating complex mixtures into their individual components. But what if you need more than just identification? Preparative HPLC takes the power of HPLC to the next level, isolating and purifying desired compounds in quantities… Read More »HPLC Prep HPLC Columns Full Guide

Introduction to Normal and Reverse Phase Chromatography Columns In High-Performance Liquid Chromatography (HPLC), the choice of column is critical to achieving optimal separation and analysis of compounds. Normal Phase (NP) and Reverse Phase (RP) chromatography columns are the two main types, each with unique characteristics,… Read More »Normal vs Reverse Phase Chromatography Column 101

Ever wondered why those peaks on your HPLC chromatogram look a little off? Asymmetry, broadening, or even extra peaks can all be signs of trouble. These “peak shape issues” can significantly impact the accuracy and reliability of your analysis. Here’s why achieving a perfect peak… Read More »HPLC Peak Shape Troubleshooting Solution Column

Overview of HPLC and C18 Columns High-Performance Liquid Chromatography (HPLC) High-performance liquid chromatography (HPLC) is an analytical technique used to separate, identify, and quantify the components in a mixture. It relies on pumps to pass a liquid mobile phase containing the sample through a stationary… Read More »C18 Guard HPLC Column Full Guide

There are numerous types of HPLC guard columns available on the market. How do you choose the right one for your HPLC system?  Let’s explore the top 10 popular guard columns in the HPLC market. We hope this information will assist you in selecting the best guard… Read More »10-HPLC Guard Column Suppliers You Must Know

PFP (Pentafluorophenyl) columns offer a unique advantage. The presence of fluorine atoms on the stationary phase enhances its affinity for aromatic compounds (compounds with ring structures). This makes PFP columns particularly effective for separating complex mixtures containing these types of molecules. In essence, HPLC with… Read More »PFP Column You Must Know

Have you ever wondered how scientists untangle complex mixtures into their individual components? Well, high-performance liquid chromatography (HPLC) is a powerful technique that achieves just that. And at the heart of this process lies a critical element: the HPLC column. What Different C8 or C18… Read More »Key Differences Between C8 and C18 Columns You Must Know

A C18 guard column is a small disposable cartridge filled with the same packing material as your main analytical column, but typically shorter in length. C18 refers to a specific type of stationary phase used in high-performance liquid chromatography (HPLC). For this Guidelines, will included… Read More »C18 Guard Column Guidelines