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High-performance liquid chromatography (HPLC) is a powerful analytical technique that separates and analyzes components in a mixture. It works by pushing a liquid sample (mobile phase) through a column packed with stationary phase. As the sample travels through the column, different components interact with the… Read More »Full Guide of HPLC Solvent Filter

High-Performance Liquid Chromatography (HPLC) is a powerful analytical technique widely used in various fields, including pharmaceuticals, environmental science, and food analysis. It separates and analyzes complex mixtures based on their chemical properties, offering high sensitivity and accuracy. However, the quality of HPLC results relies heavily… Read More »The Essential Guide to HPLC Solvent Filters: Selection, Use, and Maintenance

High-performance liquid chromatography (HPLC) is a powerful analytical technique used to separate and identify components of a mixture based on their polarity and size. It’s like a super-powered filtering system that can distinguish between even the most closely related molecules. Imagine a tiny racetrack where… Read More »Why Acetonitrile is Used as Solvent in HPLC System

In the realm of analytical chemistry, high-performance liquid chromatography (HPLC) and ultra-high-performance liquid chromatography (UHPLC) stand as two pillars of separation science. While both techniques excel in separating and identifying components within complex mixtures, they differ in their operating principles and capabilities, leading to distinct… Read More »What is the difference between HPLC and UHPLC

Exploring the Role of HPLC in the Pharmaceutical Industry In the intricate world of pharmaceutical development and manufacturing, high-performance liquid chromatography (HPLC) stands as an indispensable tool, playing a pivotal role in ensuring the safety, efficacy, and consistency of drugs. This powerful analytical technique has… Read More »Exploring the Role of HPLC in the Pharmaceutical Industry

Chromatography: A Pivotal Analytical Technique In the realm of analytical chemistry, chromatography stands as a cornerstone technique, employed to decipher the intricate composition of complex mixtures. Its versatility and adaptability have propelled it to the forefront of scientific investigations, enabling scientists to separate, identify, and… Read More »What is the difference between HPLC and other chromatography techniques (GC, LCMS, FPLC, UPLC, etc.)?

High-Performance Liquid Chromatography (HPLC), formerly referred to as high-pressure liquid chromatography, is a versatile and powerful technique widely used in analytical chemistry to separate, identify, and quantify components in complex mixtures. It has found applications in various fields, including pharmaceuticals, food science, environmental analysis, and… Read More »How Long Does HPLC Take ?

In the realm of scientific analysis, high-performance liquid chromatography (HPLC) reigns as a cornerstone technique, empowering researchers and scientists to delve into the intricate composition of complex mixtures. Its versatility and precision have propelled it to the forefront of analytical chemistry, making it an indispensable… Read More »HPLC Demystified: Your Essential Guide to High-Performance Liquid Chromatography

HPLC (high-performance liquid chromatography) is a powerful analytical technique used to separate and quantify a wide range of compounds. However, like any other analytical technique, HPLC systems can be susceptible to a variety of problems. Here is a list of the most common 30 HPLC… Read More »10 HPLC Problems and Solutions You Must Know

Do You Know When should a liquid chromatography method be set at the exact temperature ? Liquid chromatography (LC) is a powerful analytical technique used to separate, identify, and quantify components in a liquid mixture. It is based on the principle that different compounds have different… Read More »Setting Your Liquid Chromatography to the Right Temperature