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C18 Guard Columns Explained: Enhancing Performance in HPLC
1. Introduction High-Performance Liquid Chromatography (HPLC) is a powerful analytical technique widely used in various industries, including
Home » HPLC Capillary Tubing
uHPLCs is a professional manufacturer of stainless steel capillary tubing for HPLC systems.
We offer an extensive range of tubing options with inner diameters of 0.12 mm, 0.17 mm, and 0.25 mm to cater to the diverse requirements of high-performance liquid chromatography (HPLC) applications.
Our stainless steel tubing is meticulously crafted to provide superior strength, corrosion resistance, and optimal flow characteristics, ensuring precise and reliable performance in analytical processes. Each piece of tubing undergoes rigorous quality control to maintain consistent wall thickness and smooth internal surfaces, reducing the risk of blockages and enhancing separation efficiency.
We are committed to delivering exceptional products that support the advanced needs of the scientific and industrial communities. Our HPLC capillary stainless steel tubing is trusted by researchers, laboratories, and industrial operations worldwide for its unmatched quality and performance.
Volume Ring | Size | Part Number |
---|---|---|
HPLC Capillary 1/32 Inner size 0.17mm | 90 | P8F0B-00287 |
HPLC Capillary 1/32 Inner size 0.17mm | 100 | P8F0B-00326 |
HPLC Capillary 1/32 Inner size 0.17mm | 105 | P8F0B-00288 |
HPLC Capillary 1/32 Inner size 0.17mm | 150 | P8F0B-00289 |
HPLC Capillary 1/32 Inner size 0.17mm | 160 | P8F0B-00335 |
HPLC Capillary 1/32 Inner size 0.17mm | 200 | P8F0B-00336 |
HPLC Capillary 1/32 Inner size 0.17mm | 220 | P8F0B-00290 |
HPLC Capillary 1/32 Inner size 0.17mm | 230 | P8F0B-00327 |
HPLC Capillary 1/32 Inner size 0.17mm | 250 | P8F0B-00291 |
HPLC Capillary 1/32 Inner size 0.17mm | 280 | P8F0B-00337 |
HPLC Capillary 1/32 Inner size 0.17mm | 300 | P8F0B-00292 |
HPLC Capillary 1/32 Inner size 0.17mm | 320 | P8F0B-00328 |
HPLC Capillary 1/32 Inner size 0.17mm | 350 | P8F0B-00293 |
HPLC Capillary 1/32 Inner size 0.17mm | 400 | P8F0B-00338 |
HPLC Capillary 1/32 Inner size 0.17mm | 450 | P8F0B-00294 |
HPLC Capillary 1/32 Inner size 0.17mm | 550 | P8F0B-00295 |
HPLC Capillary 1/32 Inner size 0.17mm | 600 | P8F0B-00296 |
HPLC Capillary 1/32 Inner size 0.17mm | 900 | P8F0B-00297 |
HPLC Capillary 1/32 Inner size 0.12mm | 90 | P8F0B-00298 |
HPLC Capillary 1/32 Inner size 0.12mm | 105 | P8F0B-00299 |
HPLC Capillary 1/32 Inner size 0.12mm | 150 | P8F0B-00300 |
HPLC Capillary 1/32 Inner size 0.12mm | 200 | P8F0B-00329 |
HPLC Capillary 1/32 Inner size 0.12mm | 220 | P8F0B-00301 |
HPLC Capillary 1/32 Inner size 0.12mm | 250 | P8F0B-00302 |
HPLC Capillary 1/32 Inner size 0.12mm | 280 | P8F0B-00330 |
HPLC Capillary 1/32 Inner size 0.12mm | 300 | P8F0B-00303 |
HPLC Capillary 1/32 Inner size 0.12mm | 310 | P8F0B-00331 |
HPLC Capillary 1/32 Inner size 0.12mm | 350 | P8F0B-00304 |
HPLC Capillary 1/32 Inner size 0.12mm | 450 | P8F0B-00305 |
HPLC Capillary 1/32 Inner size 0.12mm | 550 | P8F0B-00306 |
HPLC Capillary 1/32 Inner size 0.12mm | 600 | P8F0B-00307 |
HPLC Capillary 1/32 Inner size 0.12mm | 700 | P8F0B-00308 |
HPLC Capillary 1/32 Inner size 0.12mm | 1000 | P8F0B-00332 |
Volume Ring | Size | Part Number |
---|---|---|
HPLC Capillary 1/16 Inner size 0.25mm | 50 | P8F0B-00311 |
HPLC Capillary 1/16 Inner size 0.25mm | 100 | P8F0B-00276 |
HPLC Capillary 1/16 Inner size 0.25mm | 150 | P8F0B-00282 |
HPLC Capillary 1/16 Inner size 0.25mm | 200 | P8F0B-00283 |
HPLC Capillary 1/16 Inner size 0.25mm | 280 | P8F0B-00284 |
HPLC Capillary 1/16 Inner size 0.25mm | 400 | P8F0B-00285 |
HPLC Capillary 1/16 Inner size 0.25mm | 500 | P8F0B-00351 |
HPLC Capillary 1/16 Inner size 0.25mm | 1000 | P8F0B-00346 |
HPLC Capillary 1/16 Inner size 0.25mm | 1500 | P8F0B-00340 |
HPLC Capillary 1/16 Inner size 0.13mm | 50 | P8F0B-00312 |
HPLC Capillary 1/16 Inner size 0.13mm | 300 | P8F0B-00341 |
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*High Precision Inner Diameters:
Available in 0.12 mm, 0.17 mm, and 0.25 mm to suit various HPLC applications.
*Superior Strength and Durability:
Made from high-quality stainless steel for enhanced durability and long-term use.
*Excellent Corrosion Resistance:
Resistant to chemical corrosion, ensuring longevity and reliability in various environments.
*Consistent Wall Thickness:
Ensures uniform performance and reduces the risk of blockages or flow inconsistencies.
*Smooth Internal Surfaces:
Minimizes turbulence and facilitates efficient, uninterrupted flow of liquids.
*High Pressure Tolerance:
Designed to withstand the high pressures commonly used in HPLC systems.
*Wide Compatibility:
Suitable for a broad range of HPLC systems and applications, providing versatility and ease of integration.
*Rigorous Quality Control:
Each piece undergoes thorough inspection and testing to ensure top-notch performance and reliability.
*Customizable Lengths:
Available in various lengths to meet specific requirements and preferences.
*Easy Installation:
Designed for straightforward installation and maintenance in HPLC setups.
Choosing the right high-pressure HPLC capillary stainless steel tubing is crucial for optimal performance and accurate results in your chromatographic system. Here are some key considerations to help you make the right choice:
By carefully considering these factors, you can select the right high-pressure HPLC capillary stainless steel tubing that meets your application’s demands, ensuring optimal performance and accurate analytical results.
Contact uHPLCs Today for Any Questions for HPLC / UHPLC
1. Introduction High-Performance Liquid Chromatography (HPLC) is a powerful analytical technique widely used in various industries, including
Investing in High-Performance Liquid Chromatography (HPLC) guard columns can lead to significant long-term cost savings
High-performance liquid chromatography (HPLC) is a versatile analytical technique used to separate, identify, and quantify
HPLC (High Performance Liquid Chromatography) relies on capillary tubing for a few key reasons:
Minimizing Band Broadening: HPLC aims to separate very similar compounds. Capillary tubing, with its small diameter, reduces the space a sample can wander within the flow path. This minimizes “band broadening” – the spreading out of the sample as it travels through the system. Sharper peaks in the final analysis lead to better separation and identification of components.
High Pressure Applications: HPLC uses high pressure to push the mobile phase (solvent) through the column. Capillary tubing, typically made from strong stainless steel, can handle this pressure without bursting.
Reduced Dead Volume: The smaller diameter of the tubing minimizes the volume of mobile phase held within it. This “dead volume” can cause unwanted mixing and band broadening. Less dead volume means sharper peaks and better resolution.
Inertness: Ideally, the tubing shouldn’t interact with the sample. Stainless steel is a good choice for most samples, but for biomolecules, PEEK lining or even bio-inert titanium tubing might be preferred to minimize unwanted adsorption.
You should use HPLC capillary tubing whenever you’re running an HPLC analysis and need:
High Resolution Separations: If you’re trying to separate very similar compounds, the narrow diameter of capillary tubing minimizes band broadening, leading to sharper peaks and better resolution in your final chromatogram.
High Pressure Applications: HPLC uses high pressures to push the mobile phase through the column. Capillary tubing, typically made from strong materials like stainless steel, can handle these pressures without bursting.
Minimal Sample Interaction: The inertness of the tubing material is important to avoid unwanted interactions between the sample and the tubing itself. Stainless steel is a good choice for most samples, but for biomolecules, PEEK lining or even bio-inert titanium tubing might be necessary to minimize adsorption.
Here are some specific scenarios where HPLC capillary tubing is essential:
Separating complex mixtures: If your sample contains a large number of closely related compounds, capillary tubing is crucial to achieve good separation and identify individual components.
Analyzing small samples: The small volume of the tubing minimizes dead volume, which is especially important when working with limited sample amounts.
HPLC with small particle size columns: Modern HPLC often utilizes columns packed with very small particles. These columns require the use of capillary tubing to minimize band broadening and maintain efficiency.
UHPLC (Ultra High Performance Liquid Chromatography): UHPLC operates at even higher pressures than traditional HPLC. Capillary tubing is essential for handling these pressures and achieving the high resolution separations characteristic of UHPLC.
In summary, HPLC capillary tubing is the standard choice for most HPLC applications due to its ability to handle high pressures, minimize band broadening, and provide inertness for sample integrity.
However, there might be some exceptions:
It’s always best to consult your instrument manual or consult with an experienced HPLC user to determine the most appropriate type of tubing for your specific application.
HPLC capillary tubing is a critical component in high-performance liquid chromatography (HPLC) systems, used to transport the sample and mobile phase through the system under high pressure.
The tubing is typically made from stainless steel, which offers high mechanical strength, chemical resistance, and durability. The importance of HPLC capillary tubing lies in its ability to maintain the integrity and efficiency of the chromatographic process. High-quality tubing ensures smooth, uninterrupted flow of liquids, minimizes dead volume, and reduces the risk of contamination or sample loss.
Proper selection of tubing dimensions and materials is crucial for achieving accurate and reproducible results in HPLC analyses.
Selecting the appropriate inner diameter (ID) for HPLC capillary tubing depends on several factors, including the sample volume, desired flow rate, and specific application requirements. Smaller IDs, such as 0.12 mm, are ideal for low flow rates and small sample volumes, providing high resolution and sensitivity. Conversely, larger IDs, such as 0.25 mm, can accommodate higher flow rates and larger sample volumes, making them suitable for preparative or high-throughput applications. It’s essential to match the tubing ID with your HPLC system’s capabilities and the nature of your analytical method to optimize performance and minimize issues such as excessive back pressure or sample dispersion.
Compatibility between HPLC capillary tubing and solvents is crucial to prevent degradation, contamination, and system failure. Key considerations include:
The surface finish of HPLC capillary tubing significantly impacts chromatographic performance. A smooth internal surface minimizes friction and turbulence, promoting laminar flow and reducing the risk of sample dispersion. This is crucial for maintaining peak shape and resolution. Rough or uneven surfaces can cause eddy currents and dead volume, leading to peak broadening, tailing, and inconsistent retention times. High-quality HPLC capillary tubing undergoes rigorous polishing and finishing processes to ensure a smooth, consistent internal surface, enhancing flow characteristics, and ensuring reliable, reproducible analytical results. Investing in tubing with superior surface finish is essential for high-precision chromatographic applications.
Proper maintenance practices are essential for extending the lifespan of HPLC capillary tubing and ensuring consistent performance. Key practices include:
Stainless steel is the preferred material for HPLC capillary tubing due to its numerous advantages, including:
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