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Revolutionary 2.1mm UHPLC Direct-Connect Guard Column Hardware

Boost your UHPLC performance with the 2.1mm Direct-Connect Guard Column – superior protection, refined separation.

The uHPLCs UPLC Direct Connection Guard Column is engineered for ultra-high-performance protection of your analytical columns. Designed with a direct-connection structure, it minimizes dead volume, eliminates the need for additional fittings, and streamlines system setup — all while extending the lifespan of your main column. Ideal for precision chromatographic analysis in pharmaceuticals, food safety, environmental testing, and biotechnology, the 2.1 mm guard column ensures excellent retention, separation, and reproducibility under high pressure conditions up to 13,000 psi (900 bar).

Features and Applications:

⭐ Key Features – 2.1 mm UPLC Direct-Connection Guard Column

Direct-Connect Design
No extra joints or tubing required — easy, tool-free installation by hand.
High Pressure Tolerance
Supports up to 13,000 psi (900 bar) for ultra-high performance LC systems.
Low Dead Volume & Back Pressure
Reduces outside-column effects and band broadening for better peak shapes.
Improves Column Life
Acts as a chemical filter to trap contaminants, reducing wear on analytical columns.
Cost-Effective Cartridge Design
Affordable replaceable cartridges reduce long-term consumable cost.
Supports a Variety of Packings
Customizable with multiple reversed-phase and specialty stationary phases:
- General-Purpose C18 (L1), 1.8 μm, pH 2–8
- 100% Water-Compatible C18, 1.8 μm
- Water-Compatible + Low pH C18, 1.8 μm, pH 0.5–8
- Wide-pH C18, 1.8 μm, pH 1–11
- General C8 (L7), Phenyl (L11), SEC (L33/L59), HILIC (L3)
Versatile Applications
Compatible with analyses of:
- APIs, natural products, flavonoids, and saponins
- Antibiotics, vitamins, hormones
- Antibodies, peptides, nucleic acids (SEC)
- Polar compounds poorly retained on standard RP columns (HILIC)

🧪 Typical Applications of the 2.1 mm UPLC Direct-Connection Guard Column

✅ General-Purpose C18 (L1)

Detection of saponins, flavonoids, polyphenols
Analysis of veterinary drug residues and hormones
Testing traditional Chinese medicinal materials and herbal preparations

💧 100% Water-Compatible C18

Separation of cephalosporin antibiotics
Analysis of water-soluble vitamins (e.g., Vitamin B complex)
Detection of small-molecule drugs like Pefloxacin mesylate and Cimetidine
Pharmaceutical QC for injections and tablets (e.g., Rifaximin, Decitabine, Docetaxel)

🧬 100% Water-Compatible + Low pH C18

Quantification of amino acid derivatives and acid-sensitive drugs
Suitable for polar acids like p-Chlorophenylacetic acid and o-Methylsalicylic acid
Used in impurity profiling and stability testing (e.g., Estriol, Nimodipine)

🧪 Wide-pH Tolerant C18 (pH 1–11)

Effective for drugs requiring strong pH stability (e.g., Pantoprazole Sodium, Imipenem)
Ideal for formulations prone to strong or irreversible adsorption
Broad-range detection from acidic to alkaline drugs in complex samples

⚗️ General-Purpose C8 (L7)

Targeting hydrophobic analytes with moderate retention
Ideal for small peptides, immunosuppressants (e.g., Tacrolimus), and herbal mixtures
Applied in the testing of OTC syrups and oral liquids (e.g., Licorice Oral Liquid)

🌿 Phenyl Phase (L11)

Specialized for aromatic and π-π interaction compounds
Analysis of botanical extracts and hormone analogs (e.g., Montelukast, Oxytetracycline)

🧫 Size Exclusion Chromatography (SEC – L33/L59)

Molecular weight profiling of:
- Monoclonal antibodies (mAbs)
- Enzymes, nucleic acids, ADCs
- Biopharmaceutical development samples

💧 HILIC – Hydrophilic Interaction Chromatography

Retention of very polar, hydrophilic substances
Analysis of substances poorly retained in standard reversed-phase LC
Quantification of glutamine–alanyl–glutamine and other small polar molecules

2.1# HPLC Guard Column Selection Chart

📌 2.1# Direct-Style HPLC Guard Column Holder

Holder Code	Inner Diameter	Cartridge Dimensions (mm)	Types	Connection Type	Applications	Material
P3K0B-00272	2.1#	2.1 × 4.0	Analytical Guard Column	Direct Connection	UHPLC Column and LC-MS System	316L Stainless Steel

📦 2.1# Direct-Style HPLC Guard Column Cartridge

Cartridge Code	Types	USP	Characteristics	pH Tolerance Range	Applications	Particle Size
P3X0B-00399	Reversed-phase	L1	General-Purpose C18	2–8	Analysis of saponins, flavonoids, polyphenols, veterinary drug residues, hormones, Chinese medicinal materials, etc.	1.8μm
P3X0B-00400	Reversed-phase	L1	100% Water Compatible C18	2–8	Cephalosporin antibiotics; water-soluble vitamins; Docetaxel; Rifaximin; Aciclovir; Nitroglycerin tablets; etc.	1.8μm
P3X0B-00401	Reversed-phase	L1	100% Water Compatible + Low pH C18	0.5–8	Gentamicin sulfate; Estriol; O-methylsalicylic acid; Nimodipine; Adenosine injection; Aspirin; etc.	1.8μm
P3X0B-00402	Reversed-phase	L1	Wide pH C18	1–11	Pantoprazole Sodium; Meropenem; Ranitidine capsules; Moxifloxacin; Octreotide Acetate; etc.	1.8μm
P3X0B-00403	Reversed-phase	L7	General-Purpose C8	2–8	Tacrolimus; Clindamycin Phosphate; Compound Licorice Oral Liquid; Rifapentine; etc.	1.8μm
P3X0B-00404	Reversed-phase	L11	Phenyl	2–8	Montelukast; Oxytetracycline; Garden Radish Seed; Sophorae Fructus Pills; etc.	1.8μm
P3X0B-00405	HILIC	L3	Hydrophilic Silica (HILIC-SiO₂)	2–8	Determination of Glutamine-Alanyl-Glutamine. For substances difficult to retain on RP columns.	1.8μm

Revolutionary 2.1mm UHPLC Direct-Connect Guard Column Hardware

Features and Applications:

⭐ Key Features – 2.1 mm UPLC Direct-Connection Guard Column