uHPLCs

1. Introduction: What Is an HPLC Frit and Why It Matters As you know, In every HPLC system, the frit is a small but critical component that determines whether your column performs smoothly—or fails prematurely. An HPLC frit is a porous metal or polymer filter,… Read More »Why HPLC Frits Clog ?

High-Performance Liquid Chromatography (HPLC) delivers reliable separation and quantification across pharmaceuticals, biological samples, natural products, food safety, and countless industries. However, analytical columns—the core of your separation performance—often face contamination and premature degradation. This is where an HPLC guard column becomes essential. Acting as a… Read More »A Complete Guide to Guard Columns, Benefits, Applications, and Best Practices

Introduction Ever struggled to decide which HPLC column to use for your analysis? You’re not alone. With so many options—different particle sizes, materials, pore diameters, and surface chemistries—choosing the right column can feel overwhelming. Yet, this choice directly determines how sharp your peaks are, how… Read More »How to Select an HPLC Column from Four Key Dimensions

Introduction Ever wondered how scientists ensure your medicine is pure, or your drinking water is safe? The answer often lies in High-Performance Liquid Chromatography (HPLC) — a powerful analytical technique capable of separating, identifying, and quantifying components in complex mixtures. Known for its high resolution,… Read More »What Are the Most Common Applications of HPLC?

Introduction Food safety is a critical priority for both manufacturers and regulators. Beyond protecting public health, it is fundamentally about meeting domestic and international requirements, maintaining smooth trade, and safeguarding brand reputation. In today’s globalized supply chains, even a single incident of non-compliance can result… Read More »The Role of HPLC in Food Safety Testing

Introduction In High-Performance Liquid Chromatography (HPLC), resolution is a key factor that determines the ability to separate and identify analytes accurately. When resolution decreases, peaks begin to overlap, quantification becomes unreliable, and valuable research time is wasted on troubleshooting. This issue is common in both… Read More »How to Prevent and Solve Resolution Loss in HPLC Workflows

Introduction Have you ever started an HPLC run only to see the system pressure shoot up, the flow rate drop, or the baseline turn unstable? In many cases, the culprit is a clogged system. Blockages in the flow path are one of the most common… Read More »Clogged Systems in HPLC: Causes, Troubleshooting, and Prevention

1. Introduction to HPLC Column Size High-Performance Liquid Chromatography (HPLC) is a cornerstone of modern analysis across pharmaceuticals, biotechnology, food safety, and environmental testing. At the heart of every separation is the HPLC column, and its size—length, internal diameter (ID), particle size, and pore size—directly… Read More »HPLC Column Size – A Complete Guide to Dimensions, Particle Size, and Applications

Introduction In High-Performance Liquid Chromatography (HPLC), the baseline represents the stability and reliability of your chromatographic system. A smooth and steady baseline ensures that peaks can be clearly identified and quantified with confidence. However, many analysts have faced the frustrating situation where the baseline shows… Read More »How to Troubleshoot Baseline Noise in HPLC

Introduction High-Performance Liquid Chromatography (HPLC) is an essential analytical technique widely used in pharmaceuticals, food safety, environmental monitoring, and other industries. Despite its robustness, even experienced operators may encounter system malfunctions or decreased column efficiency. This article outlines the 10 most common HPLC problems and… Read More »Top 10 Common HPLC Problems and How to Fix Them