High-performance liquid chromatography (HPLC) plays a critical role in analytical laboratories for the separation, identification, and quantification of compounds. One of the key indicators of HPLC performance is column efficiency. High column efficiency not only improves resolution and peak shape but also leads to faster… Read More »How to Improve Your HPLC Column Efficiency

High-performance liquid chromatography (HPLC) systems rely on a range of components, from tubing and fittings to valves and columns, to ensure accurate, reliable separations. Among the critical decisions in system setup and maintenance is the choice of materials for these components. Two of the most… Read More »Choosing Between Stainless Steel and PEEK Components in HPLC

In high-performance liquid chromatography (HPLC), accuracy and consistency are paramount. However, even with the most precise instrumentation and high-quality reagents, your analysis can be compromised by one often-overlooked factor: contamination from particulates. These microscopic invaders—originating from solvents, samples, or worn system components—can clog columns, damage… Read More »Three Essential HPLC Filters for Purity in Your Analyses

Types of Columns Used in HPLC Introduction High-performance liquid chromatography (HPLC) is a technique that is used to separate and quantify the components of a mixture. It does this by using a column that is packed with a stationary phase. The stationary phase can be… Read More »How Many Types of Columns Used in HPLC ?

What Is an HPLC Guard Column? An HPLC guard column is a short, pre-column device packed with the same or similar stationary phase as the main analytical column. It is installed between the injector and the analytical column in a high-performance liquid chromatography (HPLC) system.… Read More »Top 8 HPLC Guard Columns to Protect Your System

Introduction Getting a new HPLC column is exciting—but also important. It is a core part of your chromatography system. If you use it the right way from the beginning, it can give you better performance and last much longer. In this guide, we’ll show you… Read More »A Practical Guide to Using a New HPLC Column

Introduction In High Performance Liquid Chromatography (HPLC), peak shape is more than just visual—it directly reflects method performance and data reliability. Among various distortions, peak tailing is one of the most common and problematic, often indicating issues with system configuration, sample properties, or column condition.… Read More »Tailing Peaks in HPLC: A Practical Guide to Causes, Effects, and Solutions

High-Performance Liquid Chromatography (HPLC) has become an indispensable analytical technique in laboratories worldwide. Known for its precision, reproducibility, and ability to handle complex mixtures, HPLC plays a crucial role in ensuring the quality, safety, and consistency of products across a wide range of industries. But… Read More »What Are the Application of HPLC?

HPLC columns are essential for achieving accurate and reproducible chromatographic results. However, they have a finite lifespan. Understanding the typical lifetime of an HPLC column and the factors that influence its longevity can help maximize performance and cost-effectiveness. Typical Lifespan of an HPLC Column The… Read More »How Long Does an HPLC Column Last?

What are HPLC Columns? HPLC (High-Performance Liquid Chromatography) columns are the heart of the HPLC system, playing a crucial role in the separation, identification, and quantification of components within a mixture.  These columns contain a stationary phase, which can be either solid or liquid, that… Read More »10 Best Brand of C18 HPLC Column