uHPLCs

What is a Phenyl Column? A Phenyl Column in HPLC (High-Performance Liquid Chromatography) is a type of chromatographic column that is widely used for the separation of complex mixtures. It is characterized by the presence of phenyl functional groups bonded to the stationary phase (usually… Read More »Phenyl Column You Should Know

High-Performance Liquid Chromatography (HPLC) solvent filters play a critical role in ensuring the accuracy and reliability of your chromatographic results. These filters are designed to remove particulate matter and contaminants from solvents, preventing clogging of columns, damage to pumps, and interference with detectors. By maintaining… Read More »How to Choose HPLC Solvent Filters ?

HPLC (High-Performance Liquid Chromatography) is an essential analytical technique used in laboratories for separating, identifying, and quantifying components in a mixture. However, one common issue that can arise during HPLC analysis is the appearance of a ghost peak. In this article, we’ll dive into what… Read More »Troubleshooting Ghost Peak Problems in HPLC Systems

I. Introduction  A. Definition of Peak Purity:  Peak purity refers to the degree of separation between two peaks in a chromatogram, which is a graphical representation of the components in a sample as they elute from a column in high-performance liquid chromatography (HPLC). B. Importance… Read More »How to Calculate Peak Purity in HPLC

When it comes to high-performance liquid chromatography (HPLC), one of the key decisions you’ll face is choosing between isocratic elution and gradient elution. These two methods are essential for separating compounds in your samples, but they operate in distinctly different ways. So, how do you… Read More »Isocratic vs Gradient Elution: Which to Choose for Your HPLC Needs?

In the field of analytical chemistry, chromatography plays a vital role in separating and analyzing components in a sample. Two essential components of a chromatographic system are the guard column and the analytical column. While they are both important in achieving accurate and reliable results,… Read More »What is the Difference Between a Guard Column and Analytical Column?

Introduction High-Performance Liquid Chromatography (HPLC) is a powerful analytical technique used across various industries, from pharmaceuticals and food safety to environmental monitoring and biotechnology. One of the key components in HPLC is the stationary phase—the material that interacts with the sample as it passes through… Read More »Why C18 Column Is Widely Used in HPLC

Introduction HPLC (High-performance liquid chromatography) is an essential analytical technique widely used in chemistry, biology, and environmental science to separate mixtures into their individual components. Central to its success are two phases that interact with each other in a way that allows for the separation process:… Read More »A Complete Guide to Mobile Phase and Stationary Phase in HPLC

Introduction In HPLC, back pressure refers to the resistance the mobile phase experiences as it flows through the column. It is a key factor in ensuring proper separation and efficient analysis. While back pressure is normal, significant changes—either too high or too low—can indicate issues… Read More »Step-by-step Troubleshooting Back Pressure Issue In HPLC

Understanding Column Frits A HPLC column frit is a porous disc or barrier that holds the stationary phase (packing material) within the HPLC column while allowing the mobile phase and analytes to pass through. The frit acts as a physical barrier that retains the stationary… Read More »Full Guide Choosing Best HPLC Frit for Optimal Chromatography Performance