uHPLCs

Introduction When it comes to liquid chromatography, choosing between Reversed-Phase Chromatography (RPC) and Normal-Phase Chromatography (NPC) can be tricky. Both methods have their strengths, but the right choice depends on your sample and what you’re trying to achieve. Are you working with non-polar or moderately polar compounds?… Read More »How to Select the Right HPLC Column: NPC vs RPC

Understanding Column Back Pressure What is Column Back Pressure? High-Performance Liquid Chromatography (HPLC) is a vital technique used across industries for precise compound separation and analysis. A key factor in maintaining HPLC performance is managing <strong>column back pressure</strong>, the resistance encountered by the mobile phase… Read More »What is Column Back Pressure in HPLC: Causes and Solutions Explained

Introduction to Column Chromatography Column chromatography is a widely used laboratory technique for separating, identifying, and purifying the components of a mixture. It operates on the principle of differential partitioning, where compounds in a mixture are separated based on their interactions with two distinct phases:… Read More »Column Chromatography 101: A Clear and Simple Explanation

What is Void Volume and Its Impact Dead volume refers to the unmoving, stagnant, or unswept space in a chromatography system, which is a part of the internal volume. The International Union of Pure and Applied Chemistry (IUPAC) defines dead volume as the mobile-phase volume… Read More »Dead Volume in Chromatography and How to Fix It ?

Introduction Are you getting the best performance from your HPLC column? Whether you’re an experienced chromatographer or just starting, proper care and maintenance are crucial to achieving reliable, high-quality results. But how can you ensure your column lasts longer and maintains peak efficiency? In this… Read More »15 Tips for Care and Maintenance of Your HPLC Column

Introduction Have you ever browsed a catalog of HPLC columns, only to feel lost in a sea of endless options? You’re not alone. Selecting the right HPLC column is critical—it’s the foundation for achieving accurate, reproducible results and ensuring the success of your analytical method.… Read More »A Comprehensive Guide to Selecting HPLC Columns

What is an Inlet Solvent Filter? An inlet solvent filter is used in HPLC (High-Performance Liquid Chromatography) systems to filter solvents before they enter the system. It removes particles and impurities, preventing damage to equipment and ensuring accurate analysis. This filter helps maintain system performance… Read More »TOP8 Inlet Solvent Filter Manufacturers

Why Use Guard Column in HPLC System Guard Columns: Protecting Your HPLC Investment Guard columns are short columns placed before the analytical column in HPLC systems. They protect the analytical column by: Filtering particles: Trapping debris to prevent column clogging. Absorbing contaminants: Removing compounds that… Read More »Direct Connect or Normal Guard Column: Which Is Better?

Introduction High-Performance Liquid Chromatography (HPLC) is an essential analytical technique used in laboratories and industries for the separation, identification, and quantification of components within a mixture. The efficiency and effectiveness of HPLC rely heavily on the columns used, particularly empty HPLC column tubes, which are… Read More »5-Types of Empty HPLC Column Tubes Hardware You Must Know

The Introduction of HPLC High-Performance Liquid Chromatography (HPLC) is an advanced analytical technique used to separate, identify, and quantify components in a mixture. It was developed in the 1960s and has become a vital tool in various fields, including pharmaceuticals, environmental analysis, food safety, and… Read More »How to Deal With Peak Splitting in HPLC?