uHPLCs

I. Introduction  A. Definition of Peak Purity:  Peak purity refers to the degree of separation between two peaks in a chromatogram, which is a graphical representation of the components in a sample as they elute from a column in high-performance liquid chromatography (HPLC). B. Importance… Read More »How to Calculate Peak Purity in HPLC

When it comes to high-performance liquid chromatography (HPLC), one of the key decisions you’ll face is choosing between isocratic elution and gradient elution. These two methods are essential for separating compounds in your samples, but they operate in distinctly different ways. So, how do you… Read More »Isocratic vs Gradient Elution: Which to Choose for Your HPLC Needs?

In the field of analytical chemistry, chromatography plays a vital role in separating and analyzing components in a sample. Two essential components of a chromatographic system are the guard column and the analytical column. While they are both important in achieving accurate and reliable results,… Read More »What is the Difference Between a Guard Column and Analytical Column?

Introduction High-Performance Liquid Chromatography (HPLC) is a powerful analytical technique used across various industries, from pharmaceuticals and food safety to environmental monitoring and biotechnology. One of the key components in HPLC is the stationary phase—the material that interacts with the sample as it passes through… Read More »Why C18 Column Is Widely Used in HPLC

Introduction HPLC (High-performance liquid chromatography) is an essential analytical technique widely used in chemistry, biology, and environmental science to separate mixtures into their individual components. Central to its success are two phases that interact with each other in a way that allows for the separation process:… Read More »A Complete Guide to Mobile Phase and Stationary Phase in HPLC

Introduction In HPLC, back pressure refers to the resistance the mobile phase experiences as it flows through the column. It is a key factor in ensuring proper separation and efficient analysis. While back pressure is normal, significant changes—either too high or too low—can indicate issues… Read More »Step-by-step Troubleshooting Back Pressure Issue In HPLC

Understanding Column Frits A HPLC column frit is a porous disc or barrier that holds the stationary phase (packing material) within the HPLC column while allowing the mobile phase and analytes to pass through. The frit acts as a physical barrier that retains the stationary… Read More »Full Guide Choosing Best HPLC Frit for Optimal Chromatography Performance

Introduction When it comes to liquid chromatography, choosing between Reversed-Phase Chromatography (RPC) and Normal-Phase Chromatography (NPC) can be tricky. Both methods have their strengths, but the right choice depends on your sample and what you’re trying to achieve. Are you working with non-polar or moderately polar compounds?… Read More »How to Select the Right HPLC Column: NPC vs RPC

Understanding Column Back Pressure What is Column Back Pressure? High-Performance Liquid Chromatography (HPLC) is a vital technique used across industries for precise compound separation and analysis. A key factor in maintaining HPLC performance is managing <strong>column back pressure</strong>, the resistance encountered by the mobile phase… Read More »What is Column Back Pressure in HPLC: Causes and Solutions Explained

Introduction to Column Chromatography Column chromatography is a widely used laboratory technique for separating, identifying, and purifying the components of a mixture. It operates on the principle of differential partitioning, where compounds in a mixture are separated based on their interactions with two distinct phases:… Read More »Column Chromatography 101: A Clear and Simple Explanation