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A C18 guard column is a small disposable cartridge filled with the same packing material as your main analytical column, but typically shorter in length. C18 refers to a specific type of stationary phase used in high-performance liquid chromatography (HPLC). For this Guidelines, will included… Read More »C18 Guard Column Guidelines

1. Understanding HPLC Column Types: Reverse Phase and Normal Phase High-performance liquid chromatography (HPLC) is a separation technique used to isolate and identify components within a mixture. A key part of HPLC is the column, which contains a packing material called the stationary phase. The… Read More »5 Troubleshooting Common HPLC Column Problems and Solutions

Introduction Chromatographic columns are fundamental tools in analytical chemistry. They act as separation highways, resolving complex mixtures into their individual components. This separation allows for the identification and quantification of each component, making them vital for research, quality control, and various analytical applications. Overview of… Read More »One Way to Extend the Life of a Chromatographic Column

Welcome to uHPLCs Class! In this session, we’ll explore the captivating realm of chromatography and delve into the diversity of HPLC columns.  To shed light on the underlying principles of chromatography, let’s embark on a simple experiment featuring butter, cane sugar, and water.  Through observing how these… Read More »Full Guide 5 Types of HPLC Column

Short Answer: In most cases, installing an HPLC column upside down won’t cause major problems. HPLC columns are designed to be symmetrical, and the flow direction generally doesn’t affect their performance. Power of HPLC: Separation with Precision High-performance liquid chromatography (HPLC), also known as high-pressure… Read More »What Happens if HPLC Column is Upside Down?

High-Performance Liquid Chromatography (HPLC) 101 High-Performance Liquid Chromatography (HPLC) is a powerful technique used to separate and analyze the components of a mixture. Here’s a quick breakdown: The Process: Imagine a pressurized liquid (mobile phase) carrying your sample mixture through a tube packed with a special… Read More »How Much Do You Know C18 Reverse Phase Column ?

Before Talking Details of Types of Guard Column in HPLC, let check the role of guard columns in HPLC System and Why it is so important to selection a right guard column ?  The Role of Guard Columns in HPLC Systems Guard columns are a… Read More »Types of Guard Column Used in HPLC ?

Chromatography is a powerful technique used to separate mixtures into their individual components. It works by passing a mixture (sample) in a liquid or gas form (mobile phase) through a stationary phase (a packed column or a coated surface). The components of the mixture interact… Read More »Why HPLC column shorter than GC column?

Our customers often ask us about the aging of HPLC columns, and Chromatography Academy has done two videos dedicated to aging, and today, we will share them with you. What Mean by Aging of HPLC Column? First, we have to define what aging means, and… Read More »HPLC Column Aging All You Should Know

The Heart of HPLC System : Columns HPLC columns are the workhorses of the system. Available in various types based on the separation mechanism and the stationary phase. C18 columns are the most widely used type.   Tips:  HPLC columns are the central components of… Read More »Do You Know What is c18 Column in HPLC ?