Why Guard Column is Used in HPLC
Introduction High-Performance Liquid Chromatography (HPLC) is a widely used analytical technique in the field of
LC Column Tubing
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Looking for a supplier of high-quality PEEK tubing? Look no further! Our company produces HPLC and UPLC columns in all dimensions, customized to fit each customer’s unique needs. Our blank column kits come complete with a polished 316 stainless steel column blank, featuring a 0.1μ internal surface roughness and high gloss, which helps reduce the occurrence of groove flow and inhomogeneity of the packing layer against the wall, ultimately resulting in less broadening of the spectral band caused by the column wall. Our product line includes high-performance columns for commercial use and economic columns for laboratory testing purposes. Rest assured that our HPLC columns can be tailored to your specifications.
Our stainless steel LC column tubes are a cut above the rest, with high mechanical strength, good tenacity, and excellent corrosion resistance. Stainless steel is far less likely to break than glass and other materials. In addition, the inner wall of our liquid chromatography column tubes is polished multiple times to ensure a high degree of finish, thereby improving column efficiency and reducing the wall effect.
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LC Column Tubing 1
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Main Features
LC column tubing is an essential component of liquid chromatography (LC) systems, which are widely used in analytical chemistry to separate and analyze complex mixtures of chemicals. LC column tubing connects the LC column to other system parts, such as the injection valve, the detector, and the pump.
Here are some of the main features of LC column tubing:
Material: LC column tubing is typically made of high-quality, inert materials such as stainless steel, fused silica, or PEEK (polyetheretherketone). These materials are chosen for their chemical inertness, high-pressure resistance, and low adsorption properties.
Size: The size of the LC column tubing can vary depending on the application and the type of LC column used. Common sizes range from 0.1 mm to 4.6 mm in inner diameter, with lengths typically ranging from a few centimetres to several meters.
Compatibility: LC column tubing must be compatible with the solvents and chemicals used in the LC system. The tubing must withstand the high pressures and temperatures involved in LC separations and the corrosive nature of some solvents.
Connectors: LC column tubing is typically fitted with connectors on both ends, such as ferrules or fittings, which connect the tubing to other parts of the LC system. These connectors must be compatible with the tubing material and the other components of the system.
Cut-to-length: LC column tubing is often sold in pre-cut lengths, but it can also be purchased in longer lengths and cut to the desired length using a tubing cutter. It allows for more flexibility in designing and assembling the LC system.
Surface finish: The surface finish of the LC column tubing is important for minimizing sample adsorption and improving the reproducibility of separations. Smooth, polished surfaces are preferred to rough or scratched surfaces, which can lead to peak tailing and loss of resolution.
In summary, LC column tubing is a critical component of LC systems, connecting the column to other parts of the system and allowing for precise control over the separation and analysis of complex mixtures. The main features of LC column tubing include its material, size, compatibility, connectors, cut-to-length capability, and surface finish. By carefully selecting and using high-quality LC column tubing, scientists and researchers can improve their LC separations’ accuracy, reproducibility, and efficiency.
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Effective OEM Service
At uHPLCs, we understand that every laboratory has unique needs and requirements for their HPLC systems. That’s why we are proud to offer OEM (Original Equipment Manufacturer) services for our LC Column Tubing.
With our OEM service, we can help you design and manufacture LC Column Tubing that is specifically tailored to meet the requirements of your HPLC system.
Our experienced engineers will work closely with you to ensure that the tubing fits perfectly and functions optimally in your system. Contact us today to learn more about our OEM service and how it can benefit your laboratory.
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What Clients Say
I have been using uHPLCs' LC Column Tubing for over a year now and it has exceeded my expectations. The tubing is extremely durable and consistently produces high-quality results.
Amanda LeeCEO 
Switching to bulk order uHPLCs' LC Column Tubing has drastically improved the efficiency and speed of my experiments. I highly recommend this product to anyone in the field.
Adam CheiseDesigner 
I've tried multiple brands of LC Column Tubing, but uHPLCs' is by far the best. The precision and accuracy of my data have significantly improved since I started using this product.
Floyd StallerCo-Founder 
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Looking for high-quality uHPLCs’ LC Column Tubing and pricing information? Look no further! Contact us at sales@uhplcs.com for all your questions and concerns. Our knowledgeable team is standing by to assist you with all of your needs. Don’t wait any longer to get started – reach out to us today!
High Quality HPLC Column 316L, 316 Stainless Steel Tube Materials
Design New HPLC Columns 100% As Clients Request
Make HPLC Column Samples And Test Before Mass Products.
LC Column Tubing FAQ
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HPLC Applications in Pharmaceutical Industry
LC column tubing is a versatile component of liquid chromatography (LC) systems that can use in various applications in analytical chemistry. Here are eight common applications of LC column tubing:
- High-performance liquid chromatography (HPLC): LC column tubing is a critical component of HPLC systems, which are used for the separation and analysis of a wide range of compounds, such as pharmaceuticals, natural products, and environmental pollutants. The tubing connects the HPLC column to the other parts of the system and is available in various sizes and materials to suit different applications.
- Gas chromatography (GC): LC column tubing is also used in GC systems, which separate volatile and semi-volatile compounds based on their boiling points. In GC systems, the tubing is typically made of fused silica or stainless steel and is used to connect the GC column to the injector and detector.
- Liquid chromatography-mass spectrometry (LC-MS): LC column tubing is used in LC-MS systems, which combine the separation power of LC with the detection power of mass spectrometry. The tubing connects the LC column to the mass spectrometer and must be compatible with the solvents and chemicals used in both parts of the system.
- Protein and peptide separation: LC column tubing is used to separate and analyze proteins and peptides in biological samples. In these applications, the tubing must be made of materials compatible with the sample matrix and able to withstand the high pressures and temperatures used in protein and peptide separations.
- Quality control in the pharmaceutical industry: LC column tubing is used to control pharmaceutical products, such as drugs and drug metabolites. The tubing is used to separate and quantify the various components of a sample, ensuring that the product meets the required quality standards.
- Environmental analysis: LC column tubing is used to analyze environmental samples, such as water, soil, and air. In these applications, the tubing is used to separate and quantify pollutants and other chemicals of interest.
- Food and beverage analysis: LC column tubing is used to analyze food and beverage samples, such as wine, beer, and juice. In these applications, the tubing is used to separate and quantify the various components of the sample, such as sugars, acids, and flavour compounds.
- Forensic analysis: LC column tubing is used in forensic science to analyze samples such as blood, urine, and tissue for drugs, toxins, and other compounds. The tubing is used to separate and quantify the various components of the sample, providing valuable information for forensic investigations.
In summary, LC column tubing is a versatile component of LC systems that can be used in various applications in analytical chemistry, from pharmaceutical analysis to environmental monitoring to forensic science.
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Frequently Asked Questions
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LC Column Tubing is a small-diameter tubing used in liquid chromatography (LC) systems. The tubing connects the LC column to the LC system and delivers the mobile phase to the column. It is an essential component of the LC system, as it directly affects the separation and analysis of compounds in a sample.
LC Column Tubing can be made from a variety of materials, including stainless steel, PEEK, and fused silica. The choice of tubing material depends on the sample type, column type, and analysis conditions. Stainless steel tubing is often used in high-pressure applications, while PEEK tubing is more chemically resistant and compatible with a wider range of solvents.
The choice of inner diameter (ID) depends on the flow rate, column packing, and analysis sensitivity. A larger ID can accommodate higher flow rates but may result in a loss of resolution. A smaller ID can enhance sensitivity and resolution but may require longer analysis times.
The maximum pressure rating of LC Column Tubing depends on the tubing material and ID. High-pressure applications require tubing with a higher pressure rating to prevent rupture or leaks. It is crucial to choose tubing that can withstand the maximum pressure of the LC system to ensure the safety and accuracy of the analysis.
Proper installation of LC Column Tubing is critical to ensure optimal performance and prevent system damage. Before installation, ensure the tubing is clean and free of debris or contaminants. Use appropriate tools to cut the tubing to the desired length and ensure a tight connection between the tubing and the column and system fittings.
LC Column Tubing should be replaced regularly, as it can become contaminated or damaged over time. The replacement frequency depends on the sample type, column type, and analysis conditions. It is recommended to replace the tubing every 2-3 months for routine analysis or after any major system maintenance.
LC Column Tubing can become contaminated with sample residues or impurities over time, affecting analysis results. To clean the tubing, flush it with a suitable solvent, such as methanol or acetonitrile, before and after each use. For more thorough cleaning, a solvent or acid wash may be required, depending on the sample and analysis conditions.
Common problems associated with LC Column Tubing include leaks, blockages, and fluctuations in pressure or flow rate. Leaks can be caused by loose connections or damaged tubing and can be resolved by tightening the connections or replacing the tubing. Blockages can be caused by debris or contaminants in the tubing and can be resolved by flushing the tubing with a suitable solvent. Fluctuations in pressure or flow rate can be caused by improper tubing selection or installation and can be resolved by adjusting the tubing or system parameters.
LC Column Tubing should not be reused, as it can become contaminated or damaged over time. Reusing tubing can lead to inaccurate or inconsistent analysis results and damage the LC system. It is recommended to replace the tubing regularly and use new tubing for each analysis.
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