Hsol Amino Acid HPLC Column

Features

Built for amino acid polarity, ionization and isomer separation—with efficient peaks for routine HPLC workflows.

• High-selectivity separation — Stationary phase tuned for amino/carboxyl groups and side-chain structure; resolves isomers such as leucine/isoleucine and common derivatives (OPA / FMOC / PITC).
• Efficient, faster runs — Narrow particle-size distribution (3 μm designs) with optimized packing; theoretical plate count ≥ 15,000 N/m, high resolution and symmetric peaks (tailing factor ≤ 1.2). Typical single-sample analysis can be shortened to about 15–30 minutes.
• Broad method compatibility — Supports reversed-phase, ion-exchange and hydrophilic-interaction modes for matrices such as biofluids, food extracts, fermentation broths and drug preparations; compatible with mainstream derivatization methods.
• Stability for routine use — High-purity silica reduces metal-impurity interference. Continuous service life can reach about 500–1,000 injections with gradual efficiency loss. Polymer-matrix options are available where stronger acid/base resistance is required.
• Low detection limits — Strong fit with fluorescence and MS detection; amino acid LOD can reach about 0.1–1.0 μmol/L for trace analysis needs.

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Product Selection

Start from the catalog 4.6 × 250 mm formats, then confirm the particle size and product number.

Additional dimensions and particle sizes can be customized—contact our sales engineers.

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Specifications

Core packing and operating specifications for the Hsol Amino acid specialty column.

Product selection table

Catalog SKUs from the product specification sheet. Additional specifications can be customized.

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Applications

Use cases from the Hsol Amino specification for food, pharma, clinical and agricultural amino acid work.

Typical application focus

• Fermented food QC — Amino acid nitrogen grading (e.g. soy sauce special/first grade thresholds ≥ 0.8 / ≥ 0.7 g/100 mL) and free amino acid profiles.
• Label claim verification — Amino acid oral liquids, protein powders, collagen/glycine claims and similar nutraceutical checks.
• Pharmacopoeial purity — Support for ChP / USP-style impurity control targets (e.g. impurity content ≤ 0.5% where required by the method).

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Application Cases

Chromatogram data from product documentation using Hipsoul® (uHPLCs®) Hsol Amino, 5 μm, 120 Å, 4.6 × 250 mm.

Amino acid peak table

Test note: with the Hsol Amino 5 μm, 120 Å, 4.6 × 250 mm column under these conditions, resolution between peaks should meet the testing requirements.

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Care & Use

Activation, sample prep, mobile phase and storage guidance from the product specification.

• Activation — Before first use, flush with methanol/acetonitrile (50:50) for 30 minutes at 1.0 mL/min, then equilibrate with mobile phase until the baseline is stable.
• Sample preparation — Derivatize amino acids when required; avoid high salt/protein contamination of the bed; filter with a 0.22 μm membrane.
• Mobile phase — Reversed-phase commonly uses methanol or acetonitrile with aqueous phosphate/acetate buffers; ion-exchange often uses pH gradient elution. Avoid strongly corrosive solvents (e.g. concentrated HCl or NaOH).
• Storage — Short term (≤ 1 week): methanol/water (80:20). Long term (≥ 1 month): pure methanol; store at 4–30 °C in a cool, dry place away from direct sunlight.
• Maintenance — After use, flush with mobile phase 10–15 minutes, then methanol ~20 minutes. If pressure rises abnormally, reverse-flush with methanol at low flow (0.5 mL/min) or replace the guard column.

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Support

Technical support commitments from the Hsol Amino product documentation.

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FAQ

Answers based on the Hsol Amino product specification sheet.

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