HPLC and UHPLC columns for analytical and preparative work
Select by separation mode, sample chemistry, particle size, pore size, dimensions, pressure, and application.
Column families
The range covers routine separations, polar analytes, biological macromolecules, high-pressure work, and purification scale-up.
Reversed-phase
C18, C8, PFP, and Phenyl selectivities for broad small-molecule method requirements.
Normal-phase and HILIC
Options for polar, hydrophilic, and application-specific retention challenges.
SEC and Bio-SEC
Size-exclusion formats for macromolecular and biological sample analysis.
Preparative and specialty
Scale-up, purification, and dedicated columns for specific compounds or matrices.
Match chemistry before dimensions
Begin with analyte polarity, ionization, matrix, molecular size, and detection method. Then refine hardware and packing variables.
| Separation need | Useful starting family | Selection questions |
|---|---|---|
| Broad nonpolar to moderately polar compounds | C18 or C8 | Retention, peak shape, pH range, and aqueous compatibility |
| Polar or hydrophilic compounds | HILIC or aqueous-compatible C18 | Mobile phase, water compatibility, and selectivity |
| Aromatic or halogenated analytes | PFP or Phenyl | Alternative selectivity and matrix effects |
| Proteins and macromolecules | SEC or Bio-SEC | Molecular range, pore size, and sample compatibility |
| Isolation and purification | Preparative column | Loading, target purity, scale, and method transfer |
Configurable column variables
Standard and custom options can be evaluated against the method and instrument.
Hardware
- IDs from analytical to preparative formats
- Lengths from short screening columns to preparative hardware
- Stainless steel and relevant connection options
Packing
- Stationary-phase chemistry
- Particle and pore size
- Application-specific packing and screening
Send the analyte and method requirements
Include the sample matrix, target compounds, current column, mobile phase, detector, system pressure, and desired improvement.