Phenyl HPLC Columns
USHD Phenyl HPLC Columns
USHD Phenyl is a reversed-phase phenylpropyl column, USP L11. Retention is hydrophobic, and the phenyl ring adds π-π interaction, so aromatic selectivity differs from C18.
The published specification is 5 μm, 3 μm, and 1.8 μm, 120 Å, 320 m²/g, 12% carbon, pH 2–8. Diameters on the selection sheet are 2.1 mm, 3.0 mm, and 4.6 mm.
USHD Phenyl HPLC column
Features
A phenylpropyl reversed-phase column for aromatic compounds that a C18 phase does not separate.
- Phenylpropyl phase — Ultra-pure silica bonded with a phenylpropyl stationary phase. USP L11. The published carbon load is 12%, surface area 320 m²/g, pore size 120 Å, pH 2–8.
- Hydrophobic plus π-π — The phase keeps reversed-phase retention and adds π-π interaction between the phenyl ring and aromatic analytes. That is the selectivity difference versus a linear alkane phase such as C18.
- Endcapping — The specification notes a double endcapping process for better peak symmetry.
- 100% aqueous mobile phase — The current page says the phase can run in 100% water, for water-soluble compounds as well as conventional reversed-phase solvents.
- Three particle sizes — 5 μm, 3 μm, and 1.8 μm, all listed at 120 Å on the published specification table. Smaller particles raise resolution and backpressure.
Product Selection
Analytical columns only. Internal diameters on the sheet are 2.1 mm, 3.0 mm, and 4.6 mm. The catalog tables below are the 120 Å set. The same sheet also lists 300 Å; those catalog numbers are confirmed when you request a quote.
5 μm
Standard pressure, 120 Å
8 sizes, from 2.1×50 mm to 4.6×250 mm. No 2.1×150 mm at 5 μm.
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3 μm
Higher efficiency, 120 Å
9 sizes, from 2.1×50 mm to 4.6×150 mm, including 2.1×150 mm.
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1.8 μm
Highest efficiency, 120 Å
8 sizes, from 2.1×50 mm to 4.6×100 mm. No 4.6×150 mm or 4.6×250 mm at 1.8 μm.
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Specifications
Surface area, carbon load, and pH are the figures on the current specification table, for the 120 Å phenyl phase. Catalog numbers below are the 120 Å standard sizes.
| Phase | USP | Particle size | Pore size | Surface area | Carbon load | pH |
|---|---|---|---|---|---|---|
| USHD Phenyl | L11 | 5 μm, 3 μm, 1.8 μm | 120 Å | 320 m²/g | 12% | 2–8 |
The original table lists 4.6×250 mm, 4.6×150 mm, 4.6×100 mm, 4.6×50 mm, 3.0×100 mm, 3.0×50 mm, 2.1×100 mm, and 2.1×50 mm. The sheet adds 2.1×150 mm and 3.0×150 mm where those lengths exist for that particle size. Diameters stay at 2.1, 3.0, and 4.6 mm. This is not a preparative or semi-preparative list.
USHD Phenyl, 5 μm, 120 Å
8 standard sizes. 4.6×250 mm is listed at 5 μm. There is no 2.1×150 mm at 5 μm.
| Catalog No. | Dimension | Particle size | Pore size |
|---|---|---|---|
| P1F0B-00543 | 2.1×50 mm | 5 μm | 120 Å |
| P1F0B-00544 | 2.1×100 mm | 5 μm | 120 Å |
| P1F0B-00545 | 3.0×50 mm | 5 μm | 120 Å |
| P1F0B-00546 | 3.0×100 mm | 5 μm | 120 Å |
| P1F0B-00547 | 4.6×50 mm | 5 μm | 120 Å |
| P1F0B-00548 | 4.6×100 mm | 5 μm | 120 Å |
| P1F0B-00317 | 4.6×150 mm | 5 μm | 120 Å |
| P1F0B-00316 | 4.6×250 mm | 5 μm | 120 Å |
USHD Phenyl, 3 μm, 120 Å
9 standard sizes. Includes 2.1×150 mm. The longest 3 μm column is 4.6×150 mm.
| Catalog No. | Dimension | Particle size | Pore size |
|---|---|---|---|
| P1F0B-00538 | 2.1×50 mm | 3 μm | 120 Å |
| P1F0B-00539 | 2.1×100 mm | 3 μm | 120 Å |
| P1F0B-00759 | 2.1×150 mm | 3 μm | 120 Å |
| P1F0B-00540 | 3.0×50 mm | 3 μm | 120 Å |
| P1F0B-00321 | 3.0×100 mm | 3 μm | 120 Å |
| P1F0B-00320 | 3.0×150 mm | 3 μm | 120 Å |
| P1F0B-00541 | 4.6×50 mm | 3 μm | 120 Å |
| P1F0B-00319 | 4.6×100 mm | 3 μm | 120 Å |
| P1F0B-00318 | 4.6×150 mm | 3 μm | 120 Å |
USHD Phenyl, 1.8 μm, 120 Å
8 standard sizes. Includes 2.1×150 mm. The longest 1.8 μm column is 4.6×100 mm.
| Catalog No. | Dimension | Particle size | Pore size |
|---|---|---|---|
| P1F0B-00323 | 2.1×50 mm | 1.8 μm | 120 Å |
| P1F0B-00322 | 2.1×100 mm | 1.8 μm | 120 Å |
| P1F0B-00760 | 2.1×150 mm | 1.8 μm | 120 Å |
| P1F0B-00532 | 3.0×50 mm | 1.8 μm | 120 Å |
| P1F0B-00533 | 3.0×100 mm | 1.8 μm | 120 Å |
| P1F0B-00761 | 3.0×150 mm | 1.8 μm | 120 Å |
| P1F0B-00534 | 4.6×50 mm | 1.8 μm | 120 Å |
| P1F0B-00535 | 4.6×100 mm | 1.8 μm | 120 Å |
The selection sheet also groups a 300 Å phenyl section by the same particle sizes. Those catalog numbers are not printed in this table. Send the particle size and dimensions and we will confirm the P1F0B number before quoting. Surface area and carbon load above are the published 120 Å figures.
Applications
Uses already described on this page: samples that need aromatic selectivity in addition to hydrophobic retention.
Aromatic compounds
Polycyclic aromatic hydrocarbons, pharmaceuticals with aromatic rings, and aromatic natural products such as flavonoids and alkaloids.
Isomers
Positional isomers where π-π interaction changes the elution order. The page names vitamin A isomers and some unsaturated fatty acid isomers.
Water-soluble aromatics
Nucleobases, nucleosides, and the aromatic amino acids phenylalanine, tryptophan, and tyrosine, including methods that use a fully aqueous mobile phase.
Related: USHD C18 HPLC columns · USHD PFP columns · All HPLC columns.
Support
Send the catalog number if you have it. Otherwise send particle size, pore size, and dimensions, plus the analyte if you want a phase recommendation.
Phase selection
Stay on C18 when hydrophobic retention is enough. Move to USHD Phenyl for aromatics and isomers. Use USHD PFP when the method needs pentafluorophenyl selectivity.
120 Å catalog
The tables above are the standard 120 Å list. We confirm the P1F0B number against dimensions and particle size before quoting.
300 Å and custom sizes
300 Å phenyl sizes, and dimensions that are not in the 120 Å tables, are quoted after the catalog number is confirmed.
FAQ
Answers from the current phenyl page and the 120 Å selection list.
01 What is a phenyl HPLC column?
USHD Phenyl is a reversed-phase column with a phenylpropyl stationary phase bonded to ultra-pure silica. The published USP listing is L11. Retention is hydrophobic, like C18, and the phenyl ring also gives π-π interaction with aromatic analytes.
02 How does USHD Phenyl compare with C18?
A linear C18 chain separates mainly by hydrophobicity. USHD Phenyl keeps that retention and adds π-π interaction, so aromatic compounds and some isomers that co-elute on C18 can separate. Use phenyl when a C18 method is not giving the selectivity you need.
03 What are the published specifications?
USP L11. Particle size 5 μm, 3 μm, or 1.8 μm. The specification table lists pore size 120 Å, surface area 320 m²/g, carbon load 12%, and pH 2–8. The selection sheet also has a 300 Å section at the same analytical diameters. Those 300 Å catalog numbers are confirmed on request.
04 Which catalog number is the 4.6×250 mm column?
USHD Phenyl, 5 μm, 120 Å, 4.6×250 mm is P1F0B-00316. The 5 μm, 4.6×150 mm column is P1F0B-00317. The 3 μm and 1.8 μm lists do not include 4.6×250 mm. The longest 3 μm column on the sheet is 4.6×150 mm, P1F0B-00318. The longest 1.8 μm column is 4.6×100 mm, P1F0B-00535.
05 Are these preparative columns?
No. Every diameter on this selection sheet is 2.1 mm, 3.0 mm, or 4.6 mm. Those are analytical HPLC columns. Preparative and semi-preparative columns start at 10 mm ID and are listed on the prep HPLC columns page.
06 What compounds is USHD Phenyl used for?
The current page names aromatic compounds, including polycyclic aromatic hydrocarbons, pharmaceuticals with aromatic rings, and natural products such as flavonoids and alkaloids. It also names isomer separations, and water-soluble aromatics such as nucleobases, nucleosides, and the aromatic amino acids phenylalanine, tryptophan, and tyrosine.
07 Can it run in 100% water?
The current page says phenyl columns can be used with a mobile phase that is entirely water, which is one reason they are used for water-soluble compounds that do not dissolve in the organic solvents typical of a C18 method.
08 When is PFP or C18 the better choice?
Stay on C18 when hydrophobic retention already meets the method. Move to USHD Phenyl for aromatic selectivity and π-π interaction. USHD PFP is a different phase, USP L43, and is the alternative when halogenated compounds or the extra dipole character of pentafluorophenyl is what the separation needs.
Need a size matched to your method?
Request a USHD Phenyl Quote
Send the catalog number, or the particle size, pore size, and dimensions. We will confirm availability and pricing.
Helpful to include:
- Catalog number, if it is in the table
- Particle size (5 μm, 3 μm, or 1.8 μm) and dimensions
- 120 Å or 300 Å
- Quantity and destination
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