USHD C4-Bio HPLC Column (Macroporous, pH-Resistant for Biomolecular Analysis)
USHD C4-Bio HPLC Column is built on ultra-high purity organic-inorganic hybrid macroporous silica gel and features a proprietary C4 bonding process. Designed for the analysis of macromolecular biological samples, it provides excellent stability, batch reproducibility, and exceptional resistance to extreme pH conditions (pH 1–11). This column is ideal for analyzing proteins, peptides, ADC drugs, and monoclonal antibodies (mAbs) in pharmaceutical and biological research.
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Features:
- Hybrid Macroporous Silica Base: Utilizes high-purity organic-inorganic silica gel for robust structure and performance.
- Proprietary C4 Bonding Technology: Delivers efficient separation with strong reliability and reproducibility.
- Wide pH Tolerance (1–11): Exceptional resistance to both strong acids and alkalis for versatile biological applications.
- Ideal for Large Biomolecules: Designed for the analysis of proteins, peptides, ADC drugs, and other macromolecules.
- Supports Biopharmaceutical R&D: Suitable for molecular weight analysis in monoclonal antibody (mAb) development.
- Molecular Weight Range: Capable of separating globular proteins from 5,000 to 1,250,000 Da.
Specifications
| Property | USHD C4-Bio HPLC Column |
|---|---|
| USP | L26 |
| Pore Size Option | 5µm, 300 Å |
| Surface Area (m²/g) | 110 |
| Carbon Load (%) | 4 |
| pH Tolerance Range | 1-11 |
| Column Specifications | 4.6× 250 mm 4.6 ×150 mm |
| Features and Applications |
|
Analytical column, C18 bonded phase, C18 chromatography, C18 column, C18 HPLC column, C18 stationary phase, Chromatography column, Column packing material, High performance liquid chromatography, HPLC analysis, HPLC column, HPLC separation, Liquid chromatography column, Reverse phase column, Silica-based column
Column body
This is a measure of the distribution of particles packed in a column. In HPLC, it is preferable to have narrow particle size distribution as a narrow particle size distribution can lead to better HPLC column efficiency.
Endcap
In LC chromatography the reaction of a silylating agent with unreacted accessible silanols remaining on the silica gel surface after the initial bonding reaction of the HPLC column stationary phase. HPLC column endcapping eliminates unwanted secondary silanol interactions between compounds and the stationary phase.
PEEK frit
Longer filtration life
Consistent frit-to-frit swept volumes
Biocompatible and inert to most solvents
Suitable for low and high pressure applications.
Enhanced sealing and excellent chemical resistance.
PEEK frits can be used up to 80 ºC.
All Hot Sale HPLC Columns
Select ideal HPLC column for your lab applications based on factors like separation efficiency, pore size, pH tolerance, and specifications.
| Item | USP | Pore Size Option | Surface area (m²/g) | Carbon Load (%) | PH Tolerance Range | Column Specifications |
|---|---|---|---|---|---|---|
|
USHD C18-Pro |
L1 |
5μm, 150A 3.5μm, 150A |
200 |
10 |
2-8 |
4.6X250mm 4.6X150mm 4.6X100mm 4.6X50mm 3.0X100mm 3.0X50mm 2.1X100mm 2.1X50mm |
|
USHD C18 |
L1 |
5μm, 120A 3μm, 120A 1.8μm, 120A |
320 |
17 |
2-8 |
4.6X250mm 4.6X150mm 4.6X100mm 4.6X50mm 3.0X100mm 3.0X50mm 2.1X100mm 2.1X50mm |
|
USHD C18-AQ |
L1 |
5μm, 120A 3μm, 120A 1.8μm, 120A |
320 |
12 |
2-8 |
4.6X250mm 4.6X150mm 4.6X100mm 4.6X50mm 3.0X100mm 3.0X50mm 2.1X100mm 2.1X50mm |
|
USHD C18-AR |
L1 |
5μm, 120A 3μm, 120A 1.8μm, 120A |
320 |
10 |
0.5-8 |
4.6X250mm 4.6X150mm 4.6X100mm 4.6X50mm 3.0X100mm 3.0X50mm 2.1X100mm 2.1X50mm |
|
USHD C18-T |
L1 |
5μm, 120A 3μm, 120A 1.8μm, 120A |
320 |
14 |
1-11 |
4.6X250mm 4.6X150mm 4.6X100mm 4.6X50mm 3.0X100mm 3.0X50mm 2.1X100mm 2.1X50mm |
|
USHD C8-PRO |
L7 |
5μm, 150A 3.5μm, 150A |
200 |
7 |
2-8 |
4.6X250mm 4.6X150mm 4.6X100mm 4.6X50mm 3.0X100mm 3.0X50mm 2.1X100mm 2.1X50mm |
|
USHD C8 |
L7 |
5μm, 150A 3.5μm, 120A 1.8μm, 120A |
320 |
12 |
2-8 |
4.6X250mm 4.6X150mm 4.6X100mm 4.6X50mm 3.0X100mm 3.0X50mm 2.1X100mm 2.1X50mm |
Analytical and Semi-Prep HPLC Column Size Option
Specification（ Customizable）/ Size | Materials | Applications |
USHD C18-Pro ×30mm ; 2.1×50mm ; 2.1×75mm ; 2.1×100mm ; 2.1×125mm ; 2.1×150mm; 2.1×200mm ; 2.1×250mm ; 2.1×300mm | 316L Stainless Steel | Analytical |
3.0 ×30mm ; 3.0×50mm ; 3.0×75mm ; 3.0×100mm ; 3.0×125mm ; 3.0×150mm ； 3.0×200mm ; 3.0×250mm ; 3.0×300mm ; | 316L Stainless Steel | Analytical |
4.0×20mm ; 4.0×30mm ; 4.0×50mm ; 4.0×100mm 4.0×150mm ; ; 4.0×200mm； 4.0×250mm ; 4.0×300mm | 316L Stainless Steel | Analytical |
4.6×20mm ; 4.6×30mm ; 4.6×50mm ; 4.6×100mm 4.6×150mm ; 4.6×200mm ; 4.6×250mm ; 4.6×300mm | 316L Stainless Steel | Analytical |
7.8×150mm ; 7.8×250mm ; 7.8×300mm | 316L Stainless Steel | Semi-prep |
10×50mm ; 10×100mm ; 10×150mm 10×200mm ; 10×250mm ; 10×300mm | 316L Stainless Steel | Semi-prep |
20×50mm ; 20×100mm ; 20×150mm ; 20×200mm 20×250mm ; 20×300mm | 316L Stainless Steel | Semi-prep |
21.2×150mm ; 21.2× 250mm | 316L Stainless Steel | Semi-prep |
