USHD HILIC-Amide Hilic HPLC Column
USHD Hilic-Amide Column is a chimeric HILIC hydrophilic interaction column based on amide bonding, offering similar retention behavior to HILIC amino columns but with enhanced selectivity and significantly longer column lifespan. It provides strong retention for polar ionic compounds and excels in the separation of acidic and strongly polar analytes, especially under low-pH buffer conditions.
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Features:
- Amide Chimeric HILIC Phase: Combines amino-like separation capacity with improved selectivity and durability.
- Strong Retention of Polar Compounds: Excellent for highly polar and ionic analytes in high-organic mobile phases.
- Superior Separation of Acidic Analytes: Effectively separates acidic, strongly polar compounds under low-pH conditions.
- Extended Column Life: Offers improved chemical stability and longer service life compared to traditional HILIC amino columns.
- Wide Application Range: Ideal for complex mixtures involving highly polar or ionizable molecules in pharmaceutical, environmental, and biochemical research.
- Operating pH Range: Stable performance within pH 2–9.
Specifications
| Property | USHD Hilic-Amide HPLC Column |
|---|---|
| USP | L68 |
| Pore Size Option | 5µm, 120 Å |
| Surface Area (m²/g) | 320 |
| Carbon Load (%) | 5 |
| pH Tolerance Range | 2-9 |
| Column Specifications | 4.6× 250 mm |
Features and Applications | *Amide Chimeric HILIC Column: Offers similar separation capacity to HILIC amino columns, with improved selectivity and extended column life. *Strong Retention for Polar Ions: Effectively retains strongly polar ionic compounds in high-organic mobile phase conditions. *Enhanced Acidic Compound Separation: Delivers excellent separation performance for strongly polar acidic compounds. *Low-pH Buffer Compatibility: Capable of separating complex mixtures typically handled by amino-phase columns, even in low-pH environments |
Analytical column, C18 bonded phase, C18 chromatography, C18 column, C18 HPLC column, C18 stationary phase, Chromatography column, Column packing material, High performance liquid chromatography, HPLC analysis, HPLC column, HPLC separation, Liquid chromatography column, Reverse phase column, Silica-based column
Column body
This is a measure of the distribution of particles packed in a column. In HPLC, it is preferable to have narrow particle size distribution as a narrow particle size distribution can lead to better HPLC column efficiency.
Endcap
In LC chromatography the reaction of a silylating agent with unreacted accessible silanols remaining on the silica gel surface after the initial bonding reaction of the HPLC column stationary phase. HPLC column endcapping eliminates unwanted secondary silanol interactions between compounds and the stationary phase.
PEEK frit
Longer filtration life
Consistent frit-to-frit swept volumes
Biocompatible and inert to most solvents
Suitable for low and high pressure applications.
Enhanced sealing and excellent chemical resistance.
PEEK frits can be used up to 80 ºC.
All Hot Sale HPLC Columns
Select ideal HPLC column for your lab applications based on factors like separation efficiency, pore size, pH tolerance, and specifications.
| Item | USP | Pore Size Option | Surface area (m²/g) | Carbon Load (%) | PH Tolerance Range | Column Specifications |
|---|---|---|---|---|---|---|
|
USHD C18-Pro |
L1 |
5μm, 150A 3.5μm, 150A |
200 |
10 |
2-8 |
4.6X250mm 4.6X150mm 4.6X100mm 4.6X50mm 3.0X100mm 3.0X50mm 2.1X100mm 2.1X50mm |
|
USHD C18 |
L1 |
5μm, 120A 3μm, 120A 1.8μm, 120A |
320 |
17 |
2-8 |
4.6X250mm 4.6X150mm 4.6X100mm 4.6X50mm 3.0X100mm 3.0X50mm 2.1X100mm 2.1X50mm |
|
USHD C18-AQ |
L1 |
5μm, 120A 3μm, 120A 1.8μm, 120A |
320 |
12 |
2-8 |
4.6X250mm 4.6X150mm 4.6X100mm 4.6X50mm 3.0X100mm 3.0X50mm 2.1X100mm 2.1X50mm |
|
USHD C18-AR |
L1 |
5μm, 120A 3μm, 120A 1.8μm, 120A |
320 |
10 |
0.5-8 |
4.6X250mm 4.6X150mm 4.6X100mm 4.6X50mm 3.0X100mm 3.0X50mm 2.1X100mm 2.1X50mm |
|
USHD C18-T |
L1 |
5μm, 120A 3μm, 120A 1.8μm, 120A |
320 |
14 |
1-11 |
4.6X250mm 4.6X150mm 4.6X100mm 4.6X50mm 3.0X100mm 3.0X50mm 2.1X100mm 2.1X50mm |
|
USHD C8-PRO |
L7 |
5μm, 150A 3.5μm, 150A |
200 |
7 |
2-8 |
4.6X250mm 4.6X150mm 4.6X100mm 4.6X50mm 3.0X100mm 3.0X50mm 2.1X100mm 2.1X50mm |
|
USHD C8 |
L7 |
5μm, 150A 3.5μm, 120A 1.8μm, 120A |
320 |
12 |
2-8 |
4.6X250mm 4.6X150mm 4.6X100mm 4.6X50mm 3.0X100mm 3.0X50mm 2.1X100mm 2.1X50mm |
Analytical and Semi-Prep HPLC Column Size Option
Specification（ Customizable）/ Size | Materials | Applications |
USHD C18-Pro ×30mm ; 2.1×50mm ; 2.1×75mm ; 2.1×100mm ; 2.1×125mm ; 2.1×150mm; 2.1×200mm ; 2.1×250mm ; 2.1×300mm | 316L Stainless Steel | Analytical |
3.0 ×30mm ; 3.0×50mm ; 3.0×75mm ; 3.0×100mm ; 3.0×125mm ; 3.0×150mm ； 3.0×200mm ; 3.0×250mm ; 3.0×300mm ; | 316L Stainless Steel | Analytical |
4.0×20mm ; 4.0×30mm ; 4.0×50mm ; 4.0×100mm 4.0×150mm ; ; 4.0×200mm； 4.0×250mm ; 4.0×300mm | 316L Stainless Steel | Analytical |
4.6×20mm ; 4.6×30mm ; 4.6×50mm ; 4.6×100mm 4.6×150mm ; 4.6×200mm ; 4.6×250mm ; 4.6×300mm | 316L Stainless Steel | Analytical |
7.8×150mm ; 7.8×250mm ; 7.8×300mm | 316L Stainless Steel | Semi-prep |
10×50mm ; 10×100mm ; 10×150mm 10×200mm ; 10×250mm ; 10×300mm | 316L Stainless Steel | Semi-prep |
20×50mm ; 20×100mm ; 20×150mm ; 20×200mm 20×250mm ; 20×300mm | 316L Stainless Steel | Semi-prep |
21.2×150mm ; 21.2× 250mm | 316L Stainless Steel | Semi-prep |
