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USHD HILIC-Zil Hilic HPLC Column

USHD Hilic-ZIL Column is a hydrophilic interaction ion exchange column designed with bonded zwitterionic functionalities, enabling efficient separation of acidic, basic, and zwitterionic compounds. Extremely hydrophilic in nature, it supports 100% aqueous mobile phases and is ideal for retaining highly polar ionotropic compounds and basic drug molecules that are poorly retained in traditional reversed-phase chromatography.

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Features:

  • Zwitterionic Functional Bonding: Enables selective separation of acidic, basic, and zwitterionic analytes.
  • Extremely Hydrophilic Surface: Suitable for 100% aqueous mobile phases, providing flexibility in method development.
  • Superior Retention for Polar Compounds: Effectively retains strongly polar ionotropic substances that are not well separated in RP-LC.
  • Ideal for Basic Drug Analysis: Enhanced retention and separation of basic pharmaceutical compounds.
  • Wide pH Range (2–9): Supports stable performance across a broad pH range.
  • Versatile HILIC-Ion Exchange Mode: Combines hydrophilic interaction with ion exchange for expanded selectivity.

Specifications

Property

USHD Hilic-ZIL HPLC Column

USP/
Pore Size Option

5µm, 100 Å

Surface Area (m²/g)400
Carbon Load (%) 6
pH Tolerance Range2-9
Column Specifications

4.6× 250 mm
4.6 ×150 mm 

Features and Applications

  • Zwitterionic HILIC-Ion Exchange Column: Features bonded zwitterionic groups for separating acidic, basic, and zwitterionic compounds.
  • Extremely Hydrophilic Surface: Allows the use of 100% aqueous mobile phases for enhanced method flexibility.
  • Strong Retention of Polar Ionotropic Compounds: Ideal for analytes that are weakly or not retained in reversed-phase LC.
  • Excellent for Basic Drugs: Effectively retains and separates highly polar basic pharmaceutical molecules.

Analytical column, C18 bonded phase, C18 chromatography, C18 column, C18 HPLC column, C18 stationary phase, Chromatography column, Column packing material, High performance liquid chromatography, HPLC analysis, HPLC column, HPLC separation, Liquid chromatography column, Reverse phase column, Silica-based column

Stainless Steel Preparative HPLC Empty Column For Chemistry Lab Equipment

Column body

This is a measure of the distribution of particles packed in a column. In HPLC, it is preferable to have narrow particle size distribution as a narrow particle size distribution can lead to better HPLC column efficiency.

Endcap

In LC chromatography the reaction of a silylating agent with unreacted accessible silanols remaining on the silica gel surface after the initial bonding reaction of the HPLC column stationary phase. HPLC column endcapping eliminates unwanted secondary silanol interactions between compounds and the stationary phase.

PEEK frit

Longer filtration life
Consistent frit-to-frit swept volumes
Biocompatible and inert to most solvents
Suitable for low and high pressure applications.
Enhanced sealing and excellent chemical resistance.
PEEK frits can be used up to 80 ºC.

All Hot Sale HPLC Columns

Select ideal HPLC column for your lab applications based on factors like separation efficiency, pore size, pH tolerance, and specifications.

Item USP Pore Size Option Surface area (m²/g) Carbon Load (%) PH Tolerance Range Column Specifications
USHD C18-Pro
L1
5μm, 150A
3.5μm, 150A
200
10
2-8
4.6X250mm
4.6X150mm
4.6X100mm
4.6X50mm
3.0X100mm
3.0X50mm
2.1X100mm
2.1X50mm
USHD C18
L1
5μm, 120A
3μm, 120A
1.8μm, 120A
320
17
2-8
4.6X250mm
4.6X150mm
4.6X100mm
4.6X50mm
3.0X100mm
3.0X50mm
2.1X100mm
2.1X50mm
USHD C18-AQ
L1
5μm, 120A
3μm, 120A
1.8μm, 120A
320
12
2-8
4.6X250mm
4.6X150mm
4.6X100mm
4.6X50mm
3.0X100mm
3.0X50mm
2.1X100mm
2.1X50mm
USHD C18-AR
L1
5μm, 120A
3μm, 120A
1.8μm, 120A
320
10
0.5-8
4.6X250mm
4.6X150mm
4.6X100mm
4.6X50mm
3.0X100mm
3.0X50mm
2.1X100mm
2.1X50mm
USHD C18-T
L1
5μm, 120A
3μm, 120A
1.8μm, 120A
320
14
1-11
4.6X250mm
4.6X150mm
4.6X100mm
4.6X50mm
3.0X100mm
3.0X50mm
2.1X100mm
2.1X50mm
USHD C8-PRO
L7
5μm, 150A
3.5μm, 150A
200
7
2-8
4.6X250mm
4.6X150mm
4.6X100mm
4.6X50mm
3.0X100mm
3.0X50mm
2.1X100mm
2.1X50mm
USHD C8
L7
5μm, 150A
3.5μm, 120A
1.8μm, 120A
320
12
2-8
4.6X250mm
4.6X150mm
4.6X100mm
4.6X50mm
3.0X100mm
3.0X50mm
2.1X100mm
2.1X50mm

Analytical and Semi-Prep HPLC Column Size Option

Specification( Customizable)/ Size

Materials

Applications

USHD C18-Pro ×30mm ;  2.1×50mm ;  2.1×75mm  ; 2.1×100mm  ; 2.1×125mm  ; 2.1×150mm;  2.1×200mm 2.1×250mm 2.1×300mm

316L Stainless SteelAnalytical

3.0 ×30mm ; 3.0×50mm ;  3.0×75mm ; 3.0×100mm ; 3.0×125mm ; 3.0×150mm ; 3.0×200mm ;  3.0×250mm ;  3.0×300mm ;

316L Stainless SteelAnalytical

4.0×20mm ; 4.0×30mm ; 4.0×50mm ; 4.0×100mm

4.0×150mm ;   ;  4.0×200mm; 4.0×250mm ;  4.0×300mm

316L Stainless SteelAnalytical

4.6×20mm ; 4.6×30mm  ; 4.6×50mm ; 4.6×100mm

4.6×150mm   ; 4.6×200mm ; 4.6×250mm ; 4.6×300mm 

316L Stainless SteelAnalytical

7.8×150mm ; 7.8×250mm ;  7.8×300mm

316L Stainless SteelSemi-prep

10×50mm ; 10×100mm ;  10×150mm

10×200mm ; 10×250mm ; 10×300mm

316L Stainless SteelSemi-prep

20×50mm  ; 20×100mm ; 20×150mm  ;  20×200mm

20×250mm  ; 20×300mm

316L Stainless SteelSemi-prep

21.2×150mm ;  21.2× 250mm

316L Stainless SteelSemi-prep

Contact

Email: sales@uhplcs.com
Phone: +86 0755 28502380
51-3, Fu 'an West Road, Pinghu Street, Longgang District, Shenzhen City, 518111 China
We Will Send Back Your Inquiry Within 48-Hours

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