C18 Guard HPLC Column Full Guide
Overview of HPLC and C18 Columns High-Performance Liquid Chromatography (HPLC) High-performance liquid chromatography (HPLC) is
Hilic HPLC Column
Hilic Column Wholesale and OEM Supplier
uHPLCs is one of the best HILIC Column Wholesale and OEM suppliers, specializing in providing top-tier HILIC columns designed to meet the diverse needs of your laboratory. Our columns are crafted with precision and high-quality materials, ensuring reliable and reproducible results for your chromatographic applications.
Why Choose uHPLCs HILIC Columns
1. High Efficiency and Resolution:
Our HILIC columns are engineered to deliver sharp, well-resolved peaks, enhancing the accuracy and reliability of your analyses.
2. Superior Reproducibility:
Each column is manufactured to stringent quality standards, guaranteeing consistent performance across different batches.
3. Wide Range of Selectivities:
We offer a variety of stationary phases, allowing you to choose the most suitable column for your specific separation needs.
4. Enhanced Durability:
Constructed with robust materials, our columns withstand high pressures and extended use, providing excellent longevity and value for your investment.
5. Optimized Particle Size:
Our columns feature optimized particle sizes that improve separation efficiency and reduce analysis time.
6. Excellent Chemical Compatibility:
Compatible with a broad range of solvents and samples, our columns are versatile for various applications.
7. Custom Solutions Available:
We provide tailored solutions to meet unique separation requirements, ensuring you achieve the best possible results.
Choose uHPLCs for your HILIC columns and experience superior performance, reliability, and expert support. Contact us today to learn more about our products and how we can assist with your chromatographic needs.
Hilic HPLC Column
USHD HILIC-SiO2
Hilic HPLC Column
USHD HILIC-Amide
Hilic HPLC Column
USHD HILIC-Zil
Hilic HPLC Column
Hilic HPLC Column Specification
| Item | USP | Pore Size Option | Surface area (m²/g) | Carbon Load (%) | PH Tolerance Range | Column Specifications | Features and Application |
|---|---|---|---|---|---|---|---|
| USHD HILIC-SiO2 | L3 | 5μm, 120A; 3μm, 120A; 1.8μm, 120A; | 320 | # | 2-8 | 4.6X250mm;
4.6X150mm;
3.0X100mm;
3.0X50mm;
2.1X100mm;
2.1X50mm; | 1.) Hydrophilic interaction silica gel column, special treatment of silica gel matrix, can better retain water-soluble compounds separated by polarity differences, suitable for challenging polar analysis such as separation of highly polar substances in high water phase. 2.) It has strong stability and long column life. 3.) HILIC mode can obtain better selectivity, strong retention performance and separation effect, and can separate strongly basic polar compounds under HILIC conditions. It can also be used in reverse phase mode. |
| USHD HILIC-Amide | L68 | 5μm, 120A | 320 | 5 | 2-9 | 4.6X250mm;
4.6X150mm;
| 1.) Amide chimeric HILIC hydrophilic interaction chromatography column, with separation ability similar to HILIC amino column, but different selectivity and longer column life. 2.) Under HILIC conditions, it has better retention ability for highly polar ionic compounds dissolved in highly organic mobile phase. 3.) It has a strong separation effect on acidic and highly polar compounds, and can separate some mixed molecules that are often separated by amino phase in low pH buffer. |
| USHD HILIC-ZIL | # | 5μm, 100A | 400 | 6 | 2-9 | 4.6X250mm;
4.6X150mm; | 1.) HILIC hydrophilic interaction ion exchange chromatography column, bonded with zwitterionic functional groups
can separate solutes containing acidic, basic and/or zwitterionic substances. 2.) It is extremely hydrophilic and can use 100% aqueous mobile phase, which can effectively retain highly polar ionic compounds and basic drug molecules that are weakly or not retained in RP-LC. |
In addition to custom columns, uHPLCs also offers a wide range of standard columns for various applications such as reversed-phase, normal-phase, ion exchange, size exclusion, and HILIC. We also offers prepacked columns and accessories, such as frits, end-fittings, and Guards Columns.
uHPLCs uses state-of-the-art manufacturing techniques to produce high-quality columns that are consistent, reliable, and provide excellent performance. we have a strict quality control program to ensure that all columns meet the highest CE , SGS and UL standards for performance and reproducibility.
Contact Us For Excellent HPLC Columns
Experience the Precision and Reliability of Our High-Quality C18 HPLC Columns Today
WHY uHPLCs Hilic HPLC Column ?
Some Advantage You Should Choose uHPLCs Hilic Column:
1. High Efficiency and Resolution: uHPLCs HILIC columns are designed to deliver sharp, well-resolved peaks, ensuring precise and accurate chromatographic analyses.
2. Consistent Reproducibility: Each column is manufactured under stringent quality control standards, ensuring consistent performance and reliable results across different batches.
3. Versatile Selectivity Options: We offer a wide range of stationary phases, allowing you to choose the most suitable column for your specific separation needs, enhancing your method development flexibility.
4. Exceptional Durability: Constructed from robust, high-quality materials, our HILIC columns are built to withstand high pressures and prolonged use, providing long-lasting performance and value.
5. Optimized Particle Sizes: Featuring optimized particle sizes, our columns improve separation efficiency, reduce analysis time, and enhance overall productivity.
6. Broad Chemical Compatibility: Our HILIC columns are compatible with a wide range of solvents and sample types, making them versatile for various applications in different industries.
7. Customizable Solutions: We offer customized solutions to meet your unique separation requirements, ensuring you achieve the best possible results for your specific applications.
8. Expert Support: At uHPLCs, we provide comprehensive support and expertise to help you optimize your chromatographic processes and achieve superior performance.
Choose uHPLCs HILIC columns for their superior performance, reliability, and expert support. Contact us today to learn more about our products and how we can assist with your chromatographic needs.
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What
Clients Say
I've been using HILIC columns from uHPLCs for over a year now, and the performance has been outstanding. The high efficiency and sharp peak resolution have significantly improved the accuracy of our analyses. The durability and reproducibility of these columns are unmatched. Highly recommended!
Dr. Emily RobertsAnalytical Chemist 
The wide selectivity range of uHPLCs HILIC columns has been a game-changer for our lab. We can now tailor our separations to specific needs with ease. The columns are robust, and their longevity ensures we get the best value for our investment. The customer support from uHPLCs is also excellent, always providing helpful and timely advice.
Dr. James O'ConnorPharmaceutical Researcher 
uHPLCs HILIC columns have delivered consistent and reliable results for our quality control processes. The optimized particle sizes have reduced our analysis time, making our workflows more efficient. These columns are compatible with a broad range of solvents and samples, adding to their versatility. We couldn't be happier with our choice.
Sarah WilliamsQC Lab Manager 
The reproducibility and high efficiency of uHPLCs HILIC columns have greatly enhanced our environmental testing capabilities. We've seen significant improvements in our separation efficiency and peak resolution. The columns' durability and chemical compatibility make them ideal for our diverse sample types. A fantastic product!
Dr. Michael LeeEnvironmental Scientist
Custom solutions provided by uHPLCs have been instrumental in achieving the best possible results for our complex separation requirements. The expert support and guidance have been invaluable. The performance of the HILIC columns is consistently excellent, making them our go-to choice for all our chromatographic needs.
Jessica BrownBiotechnology Researcher Previous
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Hilic HPLC Column FAQ
Main Features of Hilic Column?
HILIC (Hydrophilic Interaction Liquid Chromatography) columns are a type of HPLC column that is specifically designed for the separation of polar compounds. Polar compounds are those that have a strong affinity for water. Traditional reversed-phase HPLC columns are not very effective at separating polar compounds, as they tend to elute (come out of the column) too quickly.
Here are some of the main features of HILIC columns:
- Hydrophilic stationary phase: HILIC columns have a stationary phase that is hydrophilic, or water-loving. This stationary phase can be made from a variety of materials, such as silica gel or bonded phases.
- High organic mobile phase: The mobile phase in HILIC chromatography is typically high in organic solvent (e.g., acetonitrile) and low in water. This is opposite to reversed-phase chromatography, where the mobile phase is typically high in water and low in organic solvent.
- Reversed elution order: Compared to reversed-phase chromatography, HILIC columns elute analytes in the opposite order. More polar analytes will elute later than less polar analytes.
- Strong retention of polar compounds: HILIC columns are able to strongly retain polar compounds that would be poorly retained by reversed-phase columns. This makes them ideal for the separation of a wide variety of polar compounds, including pharmaceuticals, natural products, and metabolites.
Here are some of the advantages of using HILIC columns:
- Improved separation of polar compounds: HILIC columns can provide better separation of polar compounds than reversed-phase columns.
- Reduced ion suppression: HILIC columns can help to reduce ion suppression, which can be a problem with some mass spectrometry techniques.
- Increased compatibility with mass spectrometry: HILIC columns are often more compatible with mass spectrometry than reversed-phase columns.
Here are some of the disadvantages of using HILIC columns:
- Higher cost: HILIC columns can be more expensive than reversed-phase columns.
- Limited range of stationary phases: There is a more limited range of stationary phases available for HILIC columns than for reversed-phase columns.
- Slower analysis times: HILIC columns can sometimes result in slower analysis times than reversed-phase columns.
Some Applications of Hilic Column
Hydrophilic interaction liquid chromatography (HILIC) is a powerful separation technique that has found numerous applications, particularly for the analysis of polar and hydrophilic compounds.
Here are some key applications of HILIC columns:
1.Separation of Biomolecules
Glycans and Glycoconjugates: HILIC is widely used for the separation and analysis of glycans, glycopeptides, and glycoproteins. It provides excellent resolution and selectivity for these highly polar compounds.
Peptides and Proteins: HILIC is effective for separating polar and hydrophilic peptides and proteins, including membrane proteins, histones, and their variants with different modifications (acetylation, methylation, phosphorylation).
2. Oligonucleotides:
HILIC shows potential for improved resolution in the analysis of complex oligonucleotides due to its ability to separate polar compounds.
Pharmaceutical and Biomedical Applications
1. Polar Drugs and Metabolites:
HILIC is suitable for analyzing polar pharmaceuticals, drug metabolites, and other small polar molecules that are poorly retained in reversed-phase LC.
2. Metabolomics and Biomarker Analysis:
HILIC is valuable for the analysis of polar metabolites, biomarkers, and other small molecules present in biological fluids and tissues.
Complementary to Reversed-Phase LC
3. Two-Dimensional LC (2D-LC):
HILIC is highly orthogonal to reversed-phase LC, making it an ideal technique for comprehensive 2D-LC separations, especially when coupled with mass spectrometry (LC-MS).
4. Fractionation and Sample Cleanup:
HILIC can be used in combination with solid-phase extraction (SPE) or other techniques to fractionate and clean up complex samples before separation by reversed-phase LC or other methods.
So, As we know, HILIC has proven to be a powerful and versatile technique, complementing traditional reversed-phase LC and expanding the range of separations possible for polar and hydrophilic compounds in various fields, including biotechnology, pharmaceuticals, and biomedical research.
Frequently Asked Questions
A HILIC (Hydrophilic Interaction Liquid Chromatography) column is a type of analytical column used in liquid chromatography that separates analytes based on their hydrophilicity. It contains a polar stationary phase that attracts and retains hydrophilic (water-loving) compounds through mechanisms like hydrogen bonding, dipole-dipole interactions, and electrostatic forces. HILIC is complementary to reversed-phase chromatography and is particularly useful for separating highly polar and ionizable compounds that are poorly retained by non-polar stationary phases.
- Excellent selectivity for polar and hydrophilic compounds: HILIC provides superior separation of highly polar analytes like glycans, peptides, nucleic acids, metabolites, and polar drugs/metabolites that are poorly retained in reversed-phase LC.
- Orthogonal selectivity to reversed-phase LC: HILIC offers an orthogonal separation mechanism, making it ideal for 2D-LC setups and comprehensive analysis when combined with reversed-phase separations.
- Compatible with mass spectrometry: HILIC mobile phases containing high organic content are MS-friendly and facilitate efficient ionization and detection of analytes.
- Improved peak shapes: For ionizable compounds, HILIC can provide sharper peaks compared to ion-exchange chromatography by reducing secondary interactions.
Common HILIC stationary phases include bare silica, amino, amide, diol, zwitterionic, and polymer-based phases. Zwitterionic phases like the ZIC-HILIC contain both positive and negative charges, reducing undesirable ionic interactions while still providing HILIC retention. Polymer-based phases offer unique selectivity and high pH stability.
Key parameters to optimize HILIC separations include mobile phase composition (acetonitrile percentage, buffer type/concentration), pH, column temperature, and flow rate. Higher acetonitrile content increases retention, while buffers control ionic interactions. Column temperature can influence selectivity. A systematic screening of conditions is often required to achieve the desired resolution.
HILIC mobile phases are quite unique compared to other chromatography techniques like reversed-phase HPLC. Here’s the breakdown:
Key Components:
- High Organic Content (60-70%+): The bulk of the mobile phase in HILIC is a strong organic solvent, typically acetonitrile (ACN). This creates a weak interaction with the polar stationary phase.
- Low Aqueous Content (3-10%+): A small percentage of water or another polar solvent like methanol is added. This forms a water-rich layer on the stationary phase for analyte partitioning.
- Buffers (Optional): Buffers can be included to control pH and influence analyte ionization and retention. Popular choices include ammonium formate and ammonium acetate due to their compatibility with MS detection and solubility in high-ACN environments.
Important Points:
- Water is the Strong Solvent: Unlike reversed-phase, where water is the weak solvent, water acts as the stronger solvent in HILIC, eluting more polar analytes later.
- Methanol Not Ideal: While methanol is a polar solvent, it’s generally not recommended in HILIC because it can disrupt the formation of the water layer.
- Mobile Phase Sensitivity: HILIC separations can be sensitive to small changes in mobile phase composition, especially the organic solvent percentage.
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