A Complete Guide to Understanding and Using HPLC Guard Columns
Introduction In the world of liquid chromatography, ensuring the longevity and efficiency of your column
HPLC Fittings and Connectors
HPLC Fittings and Connectors Factory
At uHPLCs, we pride ourselves on being a leading provider of high-performance HPLC fittings and connectors. Our products are trusted by researchers and laboratories worldwide for their quality, precision, and reliability. Beyond offering an extensive range of fittings and connectors, we are dedicated to supporting your chromatography needs with a comprehensive selection of additional lab essentials such as HPLC columns, vials, syringes, and more. Our commitment is not just to supply products but to ensure our customers have access to the best solutions available.
Whether you’re looking to upgrade your HPLC fittings or need comprehensive lab solutions, uHPLCs is your reliable partner in achieving precision and excellence in chromatography.
Stainless Steel HPLC Unions, 4-way Tees for High-Pressure UPLC Connections
Stainless Steel Straight Union for UHPLC System or Peek Top Connector
Stainless Steel HPLC Adapter for High-Pressure HPLC Connections
Stainless Steel Straight HPLC Union for High-Pressure HPLC Connections System
Peek Unions and Crosses 3-way for HPLC Connections
Peek Unions and Peek Tee 3-way for HPLC Connections
Peek Straight HPLC Union for Low Pressure Peek Tubing
UNF 10-32” Internal To M8*1.0 Thread Peek HPLC Adapter
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How to Choose Right HPLC Fittings
To choose the right HPLC fittings and connectors for your HPLC tubing and columns, consider the following factors:
- Compatibility: The fittings and connectors should be compatible with the material and dimensions of your tubing and columns.
- Pressure Rating: Ensure that the fittings and connectors can withstand the pressure required for your specific application.
- Chemical Resistance: Consider the compatibility of the fittings and connectors with the mobile phase and any other chemicals used in the system.
- Leakage: Choose fittings and connectors with low-leakage characteristics, especially if you deal with trace analysis.
- Brand and Factory Supplier: Use fittings and connectors from a reputable brand or factory directly, known for quality and reliability.
In summary, it is important to match the fittings and connectors to the requirements of your HPLC system to ensure accurate and reliable results.
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Main Features :
- Chemical resistance: HPLC fittings and connectors are materials resistant to the solvents and chemicals used in HPLC.
- Leak-proof: HPLC fittings and connectors are designed to prevent leaks and ensure a secure connection between the different components of the system.
- Durability: HPLC fittings and connectors are designed to withstand high pressure, temperature fluctuations, and other environmental conditions.
- Compatibility: HPLC fittings and connectors are available in various sizes and types to match the specific requirements of different HPLC systems.
- Ease of use: HPLC fittings and connectors are designed for easy installation, maintenance, and replacement.
- Precision: HPLC fittings and connectors are designed with precise tolerances to ensure consistent and accurate performance.
- Repeatability: HPLC fittings and connectors are designed to provide consistent and reproducible results.
- Cost-effectiveness: HPLC fittings and connectors are available at various price points to meet the different budget requirements of different users.
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What Clients Say
I recently purchased HPLC fittings and connectors from uHPLCs and I am extremely satisfied with their quality and durability. The seamless connection and leak-proof design has improved my lab's productivity and efficiency. I highly recommend uHPLCs for all your HPLC needs.
Amanda LeeCEO 
As a chromatography researcher, I have tried many HPLC fittings and connectors, but none compares to the ones from uHPLCs. The precision and consistency in their products is unmatched, making my analysis reliable and accurate every time.
Amanda LeeCEO
I have been using uHPLCs' HPLC fittings and connectors for over a year now and they have never let me down. The high-quality materials used ensure that there is no contamination or degradation of my samples during analysis.
Adam CheiseDesigner 
I have been in the chromatography industry for decades and I have never come across HPLC fittings and connectors as good as the ones from uHPLCs. Their customer service is top-notch, and they have always been helpful in answering my questions and resolving any issues I may have had.
Adam CheiseDesigner
The HPLC fittings and connectors from uHPLCs are a must-have for any laboratory. The convenience and ease of use, combined with the durability and quality, makes them the best investment for your HPLC needs. I highly recommend them to anyone in the field.
Floyd StallerCo-Founder 
Contact Us
Need high-quality HPLC fittings and connectors?
Our expert sales team is here to provide personalized recommendations.
We offer a range of top-quality fittings, including column adapters, union tees, and bulkhead fittings.
Contact us for more info and pricing. We will try helping you improve your lab’s performance with the best products available.
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HPLC Column FAQ
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Common HPLC Fittings and Connectors
As following are some most common types of HPLC fittings and connectors:
- Fingertight fittings: These are easy to use and require no tools for tightening. They are typically made of PEEK (polyetheretherketone) and are suitable for low-pressure applications up to 400 bar. Popular sizes include 1/16 inch, 1/8 inch, and ¼ inch.
- Swagelok-type fittings: These are the most common type of HPLC fitting and are available in a variety of sizes and materials. They require a wrench to tighten and are suitable for high-pressure applications up to 10,000 psi. Popular sizes include 1/16 inch, 1/8 inch, and ¼ inch.
- Flangeless fittings: These fittings are used to connect HPLC columns to other components in the system. They are available in a variety of sizes and materials, and the popular size is specific to the column being used.
- HPLC column connectors: These are specialized connectors that are used to connect HPLC columns to other components in the system. They are typically made of stainless steel and are available in a variety of sizes. Popular sizes are specific to the column and detector being used.
It is important to consult the manufacturer’s instructions when choosing HPLC fittings and connectors, as the correct size and type of fitting will depend on the specific application.
| Type | Description | Popular Sizes |
|---|---|---|
| Fingertight | Easy to use, hand-tightened, low pressure | 1/16″, 1/8″, 1/4″ |
| Swagelok-type | Most common, wrench-tightened, high pressure | 1/16″, 1/8″, 1/4″ |
| Flangeless | Connects columns to other components | Varies by column |
| HPLC column connectors | Connects columns to other components | Varies by column and detector |
HPLC Fittings and Connectors Applications
- Sample injection: HPLC fittings and connectors are used to introduce the sample into the system for analysis.
- Column selection: HPLC fittings and connectors are used to connect and change the columns as required for further analysis.
- Mobile phase delivery: HPLC fittings and connectors control and deliver the mobile phase to the column.
- Column compression and retention: HPLC fittings and connectors control the pressure and flow rate to ensure proper separation of the sample components.
- Detection: HPLC fittings and connectors connect the detector and transfer the eluted sample for analysis.
- Column temperature control: HPLC fittings and connectors are used to regulate the column’s temperature to maintain the analysis’s stability and reproducibility.
7. Column back pressure regulation: HPLC fittings and connectors regulate the back pressure to prevent column damage and ensure optimal performance.
8. Sample pre-treatment: HPLC fittings and connectors are used to prepare the sample for analysis, such as filtration or dilution.
9. Solvent recycling: HPLC fittings and connectors are used to recycle and conserve solvents.
10. Gradient formation: HPLC fittings and connectors control the formation and delivery of gradient mixtures for more complex separations.
11. Column cleaning and maintenance: HPLC fittings and connectors are used to clean and maintain the column for optimal performance.
12. Data acquisition and analysis: HPLC fittings and connectors are used to acquire and analyze the data generated during the analysis.
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Frequently Asked Questions
HPLC fittings and connectors are components used in high-performance liquid chromatography to connect tubing, pumps, valves, and other instruments in the fluidic system.
The purpose of HPLC fittings is to provide a secure and leak-free connection between different components in the HPLC system.
What is the difference between male and female HPLC fittings?
Male HPLC fittings have external threads inserted into female fittings, which have internal threads.
HPLC fittings are typically made of stainless steel, brass, or plastic materials, such as PEEK or PTFE. For Now, More and More HPLC System use the PEEK and 316L Stainless Steel Materials. Just because the cost and quality will meet most of market requires.
No, HPLC fittings are specifically designed for high-performance liquid chromatography and may not be compatible with other types of liquid chromatography.
HPLC connectors provide a secure and leak-free connection, allow for easy disconnection and reconnection of components, and reduce the risk of contamination in the fluidic system.
Yes, HPLC connectors are designed to withstand high pressure and can be used in high-pressure applications.
Yes, HPLC fittings and connectors are typically reusable, but the reuse frequency may depend on the material and type of fitting or connector.
When selecting HPLC fittings and connectors, consider the material compatibility, pressure rating, and type of connection required for your specific application.
Hotsale HPLC Products
USHD C18-T HPLC Column
USHD C18-T HPLC Column Our C18 HPLC Column is based on ultra-high purity organic-inorganic hybrid silica, designed with a proprietary bonding process for enhanced performance.
USHD C18-AR HPLC Column
USHD C18-AR HPLC Column USHD C18-AR HPLC Column features a unique bonding process with side chain steric protection, delivering excellent selectivity and exceptional isomer separation.
USHD C18-AQ HPLC Column
USHD C18-AQ HPLC Column USHD C18-AQ HPLC Column—engineered for superior HIC chromatography. With its innovative double end-capping technique and moderate carbon content, this column delivers