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c18 hplc column 4.6 and 250mm
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Home » Portfolio Items » uHPLCs C18 HPLC Column 0.1µ, 0.4µ 250 Mm × 4.6 mm
The C18 column is a durable reversed-phase HPLC column that offers longer life at extreme pH conditions and provides excellent reproducibility stability and efficient separation results.
Method | HPLC, analytical |
Brand | uHPLCs |
Phase | USHA C18 |
USP listing | L1 |
Mode | Reversed phase (RP) |
Type | USHA HPLC column (analytical) |
Hardware | Stainless steel |
Column length | 250 mm |
Column inner diameter | 4.6 mm |
Particle shape | Spherical |
Particle size | 5 µm |
Recommended application(s) | Aldehydes, Basic, neutral or acidic drugs, Derivatized amino acids, Fat-soluble vitamins, Ketones, Pesticides, Phenolic compounds, Reversed phase, RP applications HPLC |
Endcapped | Yes |
Carbon content | 17.0 % |
pH stability | 2.0–8.0 |
Pore size | 100 Å |
Temperature stability (max) | 60 °C |
Tmax (phosphate buffer) | 40 °C |
Pressure stability (max) | 400 bar |
Scope of delivery | 1x analytical HPLC column, instruction leaflet, certificate of analysis |
uHPLCs special services:
1. All columns will be tested before delivery and a column efficiency report will be provided.
2. Column loading service is available.
C18 HPLC columns are the most widely used type of column in HPLC. They are made of silica gel that has been modified with a layer of octadecylsilane (C18). This layer makes the column hydrophobic, meaning that it will preferentially retain nonpolar compounds. C18 columns are used to separate a wide variety of compounds, including organic molecules, pharmaceuticals, and environmental pollutants.
Some of the features of C18 HPLC columns include:
C18 HPLC columns are a valuable tool for a variety of applications. They are highly efficient, versatile, and robust, making them a good choice for a wide range of analytical tasks.
Here are some additional details about the features of C18 HPLC columns:
C18 HPLC columns are a valuable tool for a variety of applications. They are highly efficient, versatile, and robust, making them a good choice for a wide range of analytical tasks.
C18 HPLC columns are used in a wide variety of applications, including:
C18 HPLC columns are a versatile tool that can be used for a wide variety of applications. They are highly efficient, versatile, and robust, making them a good choice for a wide range of analytical tasks.
Here are some additional details about the applications of C18 HPLC columns:
C18 HPLC columns are a valuable tool for a variety of applications. They are highly efficient, versatile, and robust, making them a good choice for a wide range of analytical tasks.
A 0.1µ C18 HPLC column is a high-performance liquid chromatography column that uses a C18 stationary phase with a particle size of 0.1µm for high-resolution and high-efficiency separation of complex sample mixtures.
The benefits of using this column include high-resolution separation, high efficiency and sensitivity, extreme stability, and versatility. This column allows for the separation of complex mixtures with faster and more accurate separations than previously possible.
This column is ideal for applications such as peptide mapping, proteomics, and small molecule analysis, as well as other analytical and preparative chromatography applications.
Most of the HPLC detectors, such as UV-Vis, PDA, fluorescence, and mass spectrometry, can be used with a 0.1µ C18 HPLC column.
The lifespan of a 0.1µ C18 HPLC column can vary depending on the sample type and other factors. Using proper care and maintenance, this column can provide consistent and reliable performance for many samples.
No, once the column has been used, it can’t be reused, as the stationary phase and particle size may become altered or contaminated.
The column should be flushed with suitable solvent after every use and stored in a cool, dry, and clean place. Ensure that the column is not exposed to excessive heat and humidity to avoid any damage.
Selecting the right column for your application requires careful consideration of your specific requirements, including compound properties, analysis conditions, and the column specification. It’s essential to consult with a trusted supplier or manufacturer to ensure the column is appropriate for your needs.
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