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Prep HPLC Columns for Reliable Purification

High Loading. Sharp Resolution. Reliable Performance.

When precision and productivity define your separation goals, uHPLCs Prep HPLC Columns deliver unmatched performance from analytical to industrial scale.
Designed for demanding purification workflows, these columns combine high loading capacity, excellent peak resolution, and robust mechanical strength, ensuring reliable results and long service life even under high pressure and repeated use.

Built with premium-grade stainless steel and packed with high-purity silica media, each column undergoes strict QC to guarantee batch-to-batch consistency and reproducibility. Whether you’re isolating APIs, natural products, peptides, or fine chemicals — uHPLCs provides a stable, cost-effective purification solution tailored to your needs.

💡 Key Features

High Loading Capacity — optimized for preparative-scale separations
Superior Resolution — achieve sharper, cleaner peaks
Durable Construction — stable performance under high pressure
Wide Compatibility — ideal for pharmaceuticals, biotech, food, and chemicals
Customizable Options — choose dimensions, particle sizes, and bonded phases (C18, C8, PFP, Phenyl, HILIC, SEC, etc.)

⚙️ Available Sizes
10–300 mm ID | 150–1000 mm Length | 5–30 µm particle size

✨ Engineered for purity, performance, and productivity — from lab to production.

C8, C18 Prep HPLC Columns

HILIC Mode Prep HPLC Columns

Reverse Phase PFP Prep HPLC Columns

In addition to custom-designed columns, uHPLCs manufactures a comprehensive range of standard UPLC columns for diverse applications, including reversed-phase, normal-phase, ion exchange, size exclusion, and HILIC. We also produce prepacked columns and a full selection of accessories, such as frits, end-fittings, and guard columns, to support a complete chromatography workflow.

With advanced, state-of-the-art manufacturing facilities, uHPLCs has the capability to produce UPLC columns with exceptional precision, consistency, and durability. Our processes combine precision engineering, automated packing technology, and stringent testing to ensure every column delivers superior performance and reproducibility. Backed by a strict quality control program, all our columns meet and exceed CE, SGS, and UL standards, ensuring reliability and accuracy for the most demanding analytical applications.

Prep HPLC Columns Size Option

Contact Us For Excellent UPLC Columns

Experience the Precision and Reliability of Our High-Quality Columns Today

4. Experienced R&D Team

uHPLCs is proud to have a dedicated team of highly skilled scientists and engineers specializing in HPLC and UPLC research and development. With extensive industry experience, our R&D team is at the forefront of creating innovative column technologies that address the evolving demands of modern analytical science.

Leveraging deep technical expertise, they continuously optimize column design, packing methods, and material selection to enhance performance, reproducibility, and durability. This commitment ensures that every column we deliver meets the highest standards of quality, giving our customers reliable solutions for even the most challenging applications.

Trust us by Certifications & Patent

uHPLCs take great pride in the quality of our products and services. To demonstrate our commitment to excellence, we have obtained a number of certifications and patents that attest to the performance and reliability of our offerings. Our certifications include ISO, CE, and SGS standards and our patents showcase our ability to innovate in the field. We invite you to put your trust in us and discover the benefits of using our high-quality and certified products.

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Effective OEM Service

We Supply Effective UPLC Column OEM Service, please check if can meet your lab requirements.

Cater to specific customer needs and requirements
Experienced team with deep understanding of HPLC column production and application
Design, develop, and produce custom HPLC columns
Close collaboration from initial concept to final delivery
Strict quality control process and advanced technology for optimal performance and reproducibility
Flexible and efficient OEM service to tailor HPLC columns to exact requirements
Enhanced HPLC analysis results

What
Clients Say

uHPLCs Prep Columns have transformed our purification workflow. The high loading capacity and stable backpressure allow us to scale from method development to kilo-scale purification seamlessly. Peak shapes remain consistent even after dozens of runs — truly reliable performance

Pharmaceutical R&D LaboratoryGermany

We switched to uHPLCs C18 prep columns for peptide isolation and immediately noticed sharper peaks and faster run times. The column durability is excellent, and the technical support team helped us optimize flow conditions within hours. Highly recommended.

Environmental Analysis CenterUnited States

The cost-to-performance ratio of uHPLCs prep columns is outstanding. Their customized 50 mm × 250 mm C8 columns handled our hydrophobic compound purification with minimal carryover. The reproducibility between batches is exceptional.

Biotechnology CompanyJapan

We use uHPLCs preparative columns for natural product isolation. Compared to our previous brand, the resolution and baseline stability improved significantly. Even under repeated gradient elution, column life remains strong — excellent value for academic labs.

Food Safety Testing LabAustralia

uHPLCs is more than a supplier — they’re a true partner. Their OEM prep columns are consistent, high-quality, and delivered on time. Custom dimensions and bonded phases allow us to serve our own clients with confidence.

Academic Research InstituteUK

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UPLC Column FAQ

1.) What is a prep column?

A Prep HPLC Column (Preparative Column) is designed for purifying or isolating target compounds in larger quantities, rather than just analyzing them.
Compared with analytical columns, prep columns have larger internal diameters (10–300 mm) and higher sample loading capacity, allowing for gram- to kilogram-scale purification. They are widely used in pharmaceuticals, biotechnology, and fine chemicals.

2. How is a Prep HPLC Column different from an Analytical Column?

Analytical columns are used for identifying and quantifying compounds at small sample scales (micrograms to milligrams).
Prep columns, on the other hand, are built for purification and collection of compounds in much larger quantities, with higher flow rates and larger particle sizes (typically 5–30 µm).

3. What materials and phases are available for uHPLCs Prep Columns?

uHPLCs offers prep columns in 316L stainless steel, PEEK, and glass-lined bodies, packed with high-purity silica media.
Available bonded phases include C18, C8, PFP, Phenyl, HILIC, SEC, and Normal Phase — each optimized for specific separation chemistries and sample types.

4. What are the typical dimensions of a Prep HPLC Column?

uHPLCs Prep Columns are available in sizes from 10 mm to 300 mm internal diameter and 150 mm to 1000 mm length, with particle sizes ranging from 5 µm to 30 µm depending on application scale and resolution requirements.

5. What are the common applications of Prep Columns?

Prep HPLC Columns are used for purification, isolation, and compound production in:

Active pharmaceutical ingredients (APIs)
Peptides and proteins
Natural product extracts
Fine chemicals and intermediates
Food and cosmetic ingredient purification

6. Can I customize a Prep HPLC Column?

Yes. uHPLCs supports OEM and custom manufacturing — including dimensions, bonded phases, particle sizes, and end fittings — to perfectly match your purification system and application requirements.

What is Column Efficiency in UPLC ?

Column efficiency in UPLC refers to how well a column can separate different compounds into sharp, distinct peaks during chromatography.
It’s a measure of the resolving power of the column — the higher the efficiency, the narrower and better-defined the peaks, which means better separation.

How Column Efficiency is Measured

Theoretical Plates (N):
Efficiency is usually expressed as the number of theoretical plates. A “theoretical plate” is a conceptual stage where solute molecules equilibrate between the mobile and stationary phases.
- Formula:
  $\left( \frac{t_R}{W_b} \right)^2$
  where:
  t<sub>R</sub> = retention time of the peak
  W<sub>b</sub> = peak width at the base
Height Equivalent to a Theoretical Plate (HETP):
Another way is to calculate HETP:
$\frac{L}{N}$
where:
L = column length
N = theoretical plates
Smaller H values mean better efficiency.

Why Column Efficiency Matters in UPLC

Better Resolution – High efficiency means peaks are narrow and well separated, making it easier to identify and quantify compounds.
Faster Analysis – In UPLC, smaller particle sizes (1.7–1.8 µm) increase efficiency, allowing faster runs without losing resolution.
Lower Solvent Usage – Efficient columns require less mobile phase for the same resolution.
Improved Sensitivity – Narrow peaks increase the signal-to-noise ratio, improving detection limits.

How uHPLCs Achieves High Column Efficiency

Precision Packing Technology – Uniform, high-density packing of sub-2 µm particles for optimal flow paths.
Strict Particle Size Control – Tight distribution reduces band broadening.
Advanced Column Hardware – Low dead volume end-fittings and frits maintain flow uniformity.
Rigorous Quality Testing – Every column is tested for efficiency and symmetry before shipment.

How UPLC Column Storage ?

Proper storage of a UPLC column is essential to maintain performance, extend lifespan, and prevent contamination or degradation of the stationary phase. The storage procedure depends on the column type, mobile phase used, and duration of storage.

1. Short-Term Storage (Daily to Weekly)

If the column will be reused within a few days:

Flush with the initial mobile phase to remove strongly retained compounds.

Keep the column fully capped with end plugs to prevent drying and contamination.

Store upright at room temperature, away from direct sunlight or heat sources.

2. Long-Term Storage (Weeks to Months)

Reversed-Phase Columns (e.g., C18, C8, Phenyl)

Flush with 100% organic solvent (e.g., acetonitrile or methanol) to remove water and avoid microbial growth.

Fill the column completely with the storage solvent (usually ACN or Methanol) before capping tightly.

Store at room temperature in a dust-free, dry environment.

Normal-Phase Columns

Use non-polar organic solvent (e.g., hexane or isopropanol/hexane mix) for storage.

Avoid exposure to moisture — store in a desiccator or sealed bag with desiccant if possible.

HILIC Columns

Store in high-organic content (≥70% acetonitrile) to preserve the stationary phase hydration layer.

Ion Exchange & Size Exclusion Columns

Follow manufacturer’s recommended buffer or preservative for storage (often with sodium azide to prevent microbial growth).

Never allow the column to dry out — always keep it filled with liquid.

3. General Storage Tips

Always cap both ends to prevent solvent evaporation and air bubbles.

Avoid freezing unless specified by the manufacturer.

Label the column with last used mobile phase and storage solvent.

Before reusing after storage, flush with at least 10 column volumes of the mobile phase to equilibrate.

4. uHPLCs Best Practices

At uHPLCs, all UPLC columns are shipped with an appropriate storage solvent and securely capped. We recommend customers follow our included Column Care Guide, which outlines the exact flushing and storage procedures for each column chemistry. This ensures maximum performance, reproducibility, and extended service life.

Some Applications of Prep Column

Prep HPLC Columns are essential for separating and purifying compounds at preparative and production scales.
They are widely used across pharmaceutical, biotech, food, and chemical industries, enabling researchers and manufacturers to isolate high-purity materials efficiently and reproducibly.

🔬 Key Application Areas

Industry	Typical Use	Example Compounds / Targets
Pharmaceuticals	Isolation and purification of APIs, intermediates, and impurities	Active pharmaceutical ingredients, enantiomers, drug metabolites
Biotechnology	Peptide, protein, and oligonucleotide purification	Recombinant proteins, synthetic peptides, nucleic acids
Natural Products & Plant Extracts	Isolation of bioactive compounds	Alkaloids, flavonoids, terpenes, plant-derived actives
Fine & Specialty Chemicals	Separation of complex chemical mixtures	Catalysts, intermediates, high-value additives
Food & Nutraceuticals	Purification of functional ingredients	Vitamins, amino acids, sugars, antioxidants
Analytical & Research Labs	Scale-up from analytical to semi-preparative methods	Method transfer, compound isolation for structure analysis

Why Buy Prep Column From uHPLCs

1. Engineered for High-Performance Purification

Each uHPLCs Prep Column is designed for maximum loading capacity, sharp peak resolution, and stable backpressure, ensuring reliable purification from method development to industrial-scale production.

2. Precision Manufacturing & Quality Control

We use high-purity silica and 316L stainless-steel housing, packed under strict QC protocols to guarantee uniform particle distribution and batch-to-batch consistency.
Every column is pressure-tested and performance-validated before shipment.

3. Wide Range of Phases & Dimensions

From C18, C8, PFP, Phenyl, HILIC, SEC, to Normal Phase, uHPLCs offers customized dimensions (10–300 mm ID) and particle sizes (5–30 µm) to meet diverse purification needs.

4. Cost-Effective OEM / ODM Solutions

Partner with uHPLCs for private-label or OEM supply.
We provide flexible production, global delivery, and competitive pricing — without compromising performance or reliability.

5. Expert Technical Support

Our chromatography specialists assist with method transfer, scale-up optimization, and custom column selection, ensuring you achieve the best separation efficiency and yield.

✨ uHPLCs — Your Reliable Partner for Precision Purification.
📩 Contact us today: sales@uhplcs.com | 🌐 www.uhplcs.com

Frequently Asked Questions

As Follow is Some Frequently Questions People Asked

Which is better, C8 or C18 column?

It depends on your sample’s polarity.

C18 columns have longer alkyl chains and are more hydrophobic, providing stronger retention for non-polar compounds.
C8 columns have shorter chains and weaker retention, making them ideal for moderately polar compounds or when faster elution is desired.
➡️ In preparative purification, C18 is most commonly used for general-purpose separations.

What is the difference between Prep HPLC and HPLC?

HPLC (High-Performance Liquid Chromatography) is a general technique for separating compounds, mainly for analytical quantification.
Prep HPLC focuses on purifying and collecting target compounds in larger quantities (milligrams to kilograms).
Prep HPLC uses larger columns, higher flow rates, and higher loading capacity than analytical HPLC.

Why is the pre-column (guard column) used in HPLC?

A guard column (or pre-column) protects the main analytical or prep column from contamination.
It traps impurities, particulates, and strongly retained compounds, extending the lifespan and stability of the main column.

What is Prep HPLC used for?

Prep HPLC is used to isolate, purify, and collect specific compounds from mixtures.
Typical applications include purification of APIs, peptides, proteins, natural products, and fine chemicals — anywhere high purity and recovery are critical.

What is the “Rule of 3” in HPLC?

The Rule of 3 suggests that a compound’s retention factor (k) should ideally be between 2 and 10 for optimal separation.
This ensures peaks are well resolved without excessively long run times — balancing resolution and efficiency.

How to prep an HPLC sample?

Dissolve the sample in a compatible solvent.
Filter through a 0.22 or 0.45 µm membrane to remove particulates.
Adjust concentration to avoid overloading the column.
Match solvent polarity with the mobile phase to ensure consistent injection and separation.

What are the two main types of HPLC?

Normal Phase HPLC – Polar stationary phase, non-polar mobile phase.
Reverse Phase HPLC (RP-HPLC) – Non-polar stationary phase (like C18), polar mobile phase.
Most modern applications use Reverse Phase due to better reproducibility and versatility.

What are C4, C8, and C18 columns meant for?

These refer to the length of the alkyl chain bonded to the silica surface:

C4: Short chain, used for large biomolecules (proteins, peptides).
C8: Medium chain, suitable for moderately polar compounds.
C18: Long chain, ideal for non-polar or hydrophobic compounds.

What is the ODS and BDS column?

ODS (Octadecylsilane): Standard C18 phase.
BDS (Base-Deactivated Silica): A modified C18 phase where the silica surface is treated to reduce interaction with basic compounds, improving peak symmetry for amines or polar analytes.

How many types of columns are there in HPLC?

HPLC columns can be categorized by separation mode and phase type:

Reverse Phase (C18, C8, PFP, Phenyl, etc.)
Normal Phase (Silica, Amino, CN)
Ion Exchange (Cation, Anion)
Size Exclusion (SEC, GFC)
HILIC (Hydrophilic Interaction)

What is the difference between T3 and C18 columns?

Both are C18-based, but T3 columns have lower surface coverage and enhanced polar end-capping, making them more suitable for polar compounds and 100% aqueous mobile phases.
Traditional C18 columns offer stronger hydrophobic retention but may struggle with highly polar analytes.

Need Help ?

Contact uHPLCs Today for Any Questions for HPLC / UHPLC

+(86) 0755-28502380

sales@uhplcs.com

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