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https://www.youtube.com/watch?v=Lc2HfacFsD4   For students new to liquid chromatography, understanding how to read an HPLC chromatogram is crucial. In this blog, we’ll walk you through the basics of interpreting a chromatogram. 1. Understanding the Axes: Let’s begin by looking at a typical chromatogram. Y-Axis (Vertical): Represents… Read More »How to Read HPLC Data

HPLC Peek Tubing Color Code Peek tubing is a popular choice for HPLC systems due to its chemical resistance, durability, and flexibility. One of the key advantages of Peek tubing is the ability to use color coding to quickly and easily identify different types of… Read More »Peek Tubing Color Code You Should Know

  Why do HPLC mobile phase solvents need to be filtered and deaerated To answer this question, let’s first understand what a mobile phase is. Simply put, a mobile phase is a fluid in which a carrier carries a substance into and through the stationary phase. The… Read More »Why do HPLC mobile phase solvents need to be filtered and deaerated

Hydrophilic interaction (Hilic) chromatography appears to be the same as normal phase chromatography in the stationary phase. Can the same column be used for normal phase chromatography and Hilic chromatography? Regarding the mobile phase, which is close to reversed-phase chromatography, what is the difference between… Read More »What Is The Difference Between HILIC Columns VS Normal Reverse Columns

Synthetic chemistry is inseparable from solvents, such as reaction media, HPLC column purification as eluent, extraction, etc. TLC spot plates require us to configure the unfolding agent, which then requires us to understand the nature and polarity of the various solvents. Polarity Polarity is the degree to… Read More »Comparison Of The Polarity Of Organic Solvents

Maximize Column Efficiency Using a Single Guard Column One guard column can solve most of the problems of the column and can greatly extend the use of the column time! Why did the column break down after only three months of use? The sample is… Read More »Maximize Column Efficiency Using a Single Guard Column

Uplc vs HPLC: Understanding the Differences and Advantages Ultra-high-performance liquid chromatography (UPLC) is a type of liquid chromatography that operates at a higher pressure than traditional HPLC. It is designed to perform faster and more efficiently, with higher resolution and sensitivity. UPLC uses smaller particle-size… Read More »Uplc vs HPLC,All Difference You Must Know

How to Calculate HPLC Column Volume High-Performance Liquid Chromatography (HPLC) is a widely used analytical technique for separating and analyzing complex mixtures. The column is the most critical component in the HPLC system, and its volume plays a crucial role in determining the separation efficiency… Read More »How to Calculate HPLC Column Volume

How to Calculate Concentration from Peak Area in HPLC High-Performance Liquid Chromatography (HPLC) is a powerful analytical technique used in various industries, including pharmaceuticals, food and beverage, and environmental analysis. HPLC aims to separate and identify individual components in a mixture and quantify their concentrations.… Read More »How to Calculate Concentration from Peak Area HPLC

Introduction High-performance liquid chromatography (HPLC) is a powerful analytical technique used to separate, identify, and quantify the components in a sample. HPLC analysis generates large quantities of data, which can be overwhelming for beginners. However, with the right tools and knowledge, analyzing HPLC data can… Read More »How to Analyze HPLC Data