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Types of Columns Used in HPLC Why use HPLC Columns ? High-Performance Liquid Chromatography (HPLC) is a common analytical technique used in chemistry, biochemistry, and other sciences to separate, identify, and quantify compounds. The use of columns is crucial in any HPLC laboratory as they… Read More »HPLC Column Types: A Beginner’s Guide

Similarities between High-Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC) In the last news, we  discussed “What Are The Differences Between Gas Chromatography (GC) And High-Performance Liquid Chromatography (HPLC)?” (Click here to see it ). Today, we are discussing the common function between HPLC and… Read More »Similarities between High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC)

https://www.youtube.com/watch?v=Lc2HfacFsD4   For students new to liquid chromatography, understanding how to read an HPLC chromatogram is crucial. In this blog, we’ll walk you through the basics of interpreting a chromatogram. 1. Understanding the Axes: Let’s begin by looking at a typical chromatogram. Y-Axis (Vertical): Represents… Read More »How to Read HPLC Data

HPLC Peek Tubing Color Code Peek tubing is a popular choice for HPLC systems due to its chemical resistance, durability, and flexibility. One of the key advantages of Peek tubing is the ability to use color coding to quickly and easily identify different types of… Read More »Peek Tubing Color Code You Should Know

  Why do HPLC mobile phase solvents need to be filtered and deaerated To answer this question, let’s first understand what a mobile phase is. Simply put, a mobile phase is a fluid in which a carrier carries a substance into and through the stationary phase. The… Read More »Why do HPLC mobile phase solvents need to be filtered and deaerated

Hydrophilic interaction (Hilic) chromatography appears to be the same as normal phase chromatography in the stationary phase. Can the same column be used for normal phase chromatography and Hilic chromatography? Regarding the mobile phase, which is close to reversed-phase chromatography, what is the difference between… Read More »What Is The Difference Between HILIC Columns VS Normal Reverse Columns

Synthetic chemistry is inseparable from solvents, such as reaction media, HPLC column purification as eluent, extraction, etc. TLC spot plates require us to configure the unfolding agent, which then requires us to understand the nature and polarity of the various solvents. Polarity Polarity is the degree to… Read More »Comparison Of The Polarity Of Organic Solvents

Maximize Column Efficiency Using a Single Guard Column One guard column can solve most of the problems of the column and can greatly extend the use of the column time! Why did the column break down after only three months of use? The sample is… Read More »Maximize Column Efficiency Using a Single Guard Column