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Hydrophilic interaction (Hilic) chromatography appears to be the same as normal phase chromatography in the stationary phase. Can the same column be used for normal phase chromatography and Hilic chromatography? Regarding the mobile phase, which is close to reversed-phase chromatography, what is the difference between… Read More »What Is The Difference Between HILIC Columns VS Normal Reverse Columns

Synthetic chemistry is inseparable from solvents, such as reaction media, HPLC column purification as eluent, extraction, etc. TLC spot plates require us to configure the unfolding agent, which then requires us to understand the nature and polarity of the various solvents. Polarity Polarity is the degree to… Read More »Comparison Of The Polarity Of Organic Solvents

Maximize Column Efficiency Using a Single Guard Column One guard column can solve most of the problems of the column and can greatly extend the use of the column time! Why did the column break down after only three months of use? The sample is… Read More »Maximize Column Efficiency Using a Single Guard Column

Uplc vs HPLC: Understanding the Differences and Advantages Ultra-high-performance liquid chromatography (UPLC) is a type of liquid chromatography that operates at a higher pressure than traditional HPLC. It is designed to perform faster and more efficiently, with higher resolution and sensitivity. UPLC uses smaller particle-size… Read More »Uplc vs HPLC,All Difference You Must Know

The Importance of Proper Cleaning of Flow Cells for HPLC As a scientist or researcher working with high performance liquid chromatography (HPLC), you know the importance of keeping your equipment well maintained. One of the most important components of HPLC is the flow cell. A… Read More »Expert Tips for Cleaning Your Flow Cell HPLC

I. Introduction A. Definition of Peak Purity: Peak purity refers to the degree of separation between two peaks in a chromatogram, which is a graphical representation of the components in a sample as they elute from a column in high-performance liquid chromatography (HPLC). B. Importance… Read More »How to Calculate Peak Purity in HPLC

How to Calculate HPLC Column Volume High-Performance Liquid Chromatography (HPLC) is a widely used analytical technique for separating and analyzing complex mixtures. The column is the most critical component in the HPLC system, and its volume plays a crucial role in determining the separation efficiency… Read More »How to Calculate HPLC Column Volume

How to Calculate Concentration from Peak Area in HPLC High-Performance Liquid Chromatography (HPLC) is a powerful analytical technique used in various industries, including pharmaceuticals, food and beverage, and environmental analysis. HPLC aims to separate and identify individual components in a mixture and quantify their concentrations.… Read More »How to Calculate Concentration from Peak Area HPLC

Introduction High-performance liquid chromatography (HPLC) is a powerful analytical technique used to separate, identify, and quantify the components in a sample. HPLC analysis generates large quantities of data, which can be overwhelming for beginners. However, with the right tools and knowledge, analyzing HPLC data can… Read More »How to Analyze HPLC Data

High-Performance Liquid Chromatography (HPLC) is a powerful analytical technique used in a wide range of applications. HPLC systems are used to separate, identify, and quantify the individual components of a complex mixture. In this blog post, we will explore the basics of HPLC systems, why… Read More »How Much Does An HPLC System Cost