Tailing Peaks in HPLC: A Practical Guide to Causes, Effects, and Solutions
Introduction In High Performance Liquid Chromatography (HPLC), peak shape is more than just visual—it directly reflects method performance and data reliability. Among various distortions, peak tailing is one of the most common and problematic, often indicating issues with system configuration, sample properties, or column condition. This guide provides a clear and practical roadmap to understanding…