Retention Time Drift in HPLC: Causes, Corrections, and Prevention
1. Introduction High-Performance Liquid Chromatography (HPLC) is a powerful and widely used analytical technique in chemistry, pharmaceuticals, environmental testing, food safety, and many other fields. It allows scientists to separate, identify, and quantify components in complex mixtures with high precision and accuracy. As a fundamental part of HPLC analysis, retention time (RT) serves as one…