Troubleshooting

Prevent and recover HPLC resolution loss by isolating column, method, and sample-matrix causes.

Introduction Have you ever started an HPLC run only to see the system pressure shoot up, the flow rate drop, or the baseline turn unstable? In many cases, the culprit is a clogged system. Blockages in the flow path are one of the most common issues chromatographers face. Not only do they interrupt your analysis,…

Introduction In High-Performance Liquid Chromatography (HPLC), the baseline represents the stability and reliability of your chromatographic system. A smooth and steady baseline ensures that peaks can be clearly identified and quantified with confidence. However, many analysts have faced the frustrating situation where the baseline shows irregular fluctuations, commonly referred to as baseline noise. So why…

Introduction High-Performance Liquid Chromatography (HPLC) is an essential analytical technique widely used in pharmaceuticals, food safety, environmental monitoring, and other industries. Despite its robustness, even experienced operators may encounter system malfunctions or decreased column efficiency. This article outlines the 10 most common HPLC problems and provides practical solutions to help laboratory personnel troubleshoot and optimize…

Introduction High Performance Liquid Chromatography (HPLC) is an essential analytical technique widely used in pharmaceutical, environmental, food, and chemical industries for the separation, identification, and quantification of complex mixtures. Its accuracy, sensitivity, and repeatability make it a cornerstone in modern analytical laboratories. At the heart of any HPLC system lies the column—the component responsible for…

1. Introduction High-Performance Liquid Chromatography (HPLC) is a powerful and widely used analytical technique in chemistry, pharmaceuticals, environmental testing, food safety, and many other fields. It allows scientists to separate, identify, and quantify components in complex mixtures with high precision and accuracy. As a fundamental part of HPLC analysis, retention time (RT) serves as one…

HPLC (High-Performance Liquid Chromatography) is an essential analytical technique used in laboratories for separating, identifying, and quantifying components in a mixture. However, one common issue that can arise during HPLC analysis is the appearance of a ghost peak. In this article, we’ll dive into what ghost peaks are, the potential hazards they pose, the causes…

Introduction In HPLC, back pressure refers to the resistance the mobile phase experiences as it flows through the column. It is a key factor in ensuring proper separation and efficient analysis. While back pressure is normal, significant changes—either too high or too low—can indicate issues with the system, such as column blockages, leaks, or improper…

Understanding Column Back Pressure What is Column Back Pressure? High-Performance Liquid Chromatography (HPLC) is a vital technique used across industries for precise compound separation and analysis. A key factor in maintaining HPLC performance is managing <strong>column back pressure</strong>, the resistance encountered by the mobile phase as it flows through the system. Excessive back pressure can…

What is Void Volume and Its Impact Dead volume refers to the unmoving, stagnant, or unswept space in a chromatography system, which is a part of the internal volume. The International Union of Pure and Applied Chemistry (IUPAC) defines dead volume as the mobile-phase volume that solutes experience outside of the column. Void volume, another…