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HPLC is a workhorse technique in analytical chemistry. It separates and identifies compounds in a mixture using a pressurized solvent and a specialized column packed with microscopic particles. Imagine a tiny maze specifically designed to trap certain molecules based on their size and interactions. The separated… Read More »How to Remove Air Bubbles from HPLC Columns: A Comprehensive Guide

 The HPLC column is an important component of liquid chromatography because it is the column responsible for the separation of analytes from the matrix. Protecting and maintaining the column ensures not only reliable results but also trouble-free operation.  We all know that HPLC columns have a limited life, but… Read More »Principle of HPLC Guard Column

Liquid chromatography (LC) is a powerful analytical technique widely used in various industries, including pharmaceuticals, forensics, environmental science, and food and beverage analysis. It separates and identifies components within a complex mixture by passing a liquid mobile phase through a stationary phase packed within a… Read More »Why Use a Guard Column for Liquid Chromatography System ?

Xylitol is a naturally occurring sugar alcohol found in small amounts in various fruits and vegetables, like berries, plums, and corn. However, it can also be commercially produced from birch or hardwood trees. Despite its name, xylitol isn’t technically an alcohol but a polyol, a… Read More »How HPLC Purifies Xylitol : From Plant to Sweetener

High-performance liquid chromatography (HPLC) holds a prominent position in the realm of analytical chemistry. Imagine a complex mixture, a scientific puzzle holding valuable information within. HPLC acts as the key, meticulously separating and identifying each individual component. Its role extends beyond mere identification, allowing for… Read More »Total Guide of C18 Reverse Phase Column

A range of methods can detect petroleum composition and its products. Still, comparing many methods has led to the conclusion that high-performance liquid chromatography separation and mass spectrometry detection is currently the most effective analytical method and representative of the composition of the structural families… Read More »Innovative HPLC Applications and Product Analysis in the Petroleum Industry

Dead Volume and its Role in HPLC Systems In high-performance liquid chromatography (HPLC), dead volume refers to the overall volume of the system that the mobile phase flows through but doesn’t contribute to the separation process. Think of it as the nooks and crannies –… Read More »The Dead Volume Advantage: Thriving Beyond Peak Constraints

 Sample pretreatment is the process of sample preparation and extraction, purification and concentration of the components to be measured in the sample.  Sample pretreatment aims to eliminate matrix interference, protect the instrument, and improve the assay’s sensitivity, selectivity, accuracy, and precision. Sample Pretreatment Method Sample… Read More »4 Tips for Sample Pretreatment in Liquid Chromatography

Mobile phase composition plays a crucial role in most separations. In most cases, mobile phase is mixed of water and organic solvents. In isocratic operation the composition is pre- decided and remains unchanged during the analytical run.  On the other hand in gradient elution the final… Read More »Key Strategies for Choosing the Right Mobile Phase in Chromatography