Why C18 HPLC column so popular used in market ?
Why C18 HPLC Columns Reign Supreme
C18 HPLC columns are the workhorses of the analytical chemistry laboratory. Their widespread popularity stems from a combination of factors:
Versatility
1. Wide range of compound compatibility:
C18 columns effectively retain a vast array of compounds, from non-polar to moderately polar substances.
2. Adaptability to different mobile phases:
By adjusting the composition of the mobile phase, analysts can fine-tune the retention and separation of target analytes.
Reliability and Robustness
1.Consistent performance:
C18 columns exhibit excellent reproducibility, ensuring reliable results from batch to batch.
2. Durability:
They can withstand harsh mobile phase conditions and repeated use, making them cost-effective in the long run.
Hydrophobic Nature
1.Strong retention of non-polar compounds:
The C18 bonded phase effectively interacts with hydrophobic molecules, leading to efficient separation.
2. Enhanced peak shape:
The hydrophobic interactions contribute to sharper peak shapes, improving detection sensitivity.
Established Methodology
1. Extensive literature and applications:
A wealth of information and methods are available for C18 columns, making method development and optimization relatively straightforward.
2. Industry standards:
Many regulatory bodies and industries have adopted C18 columns as the standard for various analyses.
So to choose the right C18 Chromatography Column will be the important for your HPLC System and Project.
Here we list ten of the best C18 HPLC column brands, along with detailed insights into their product features and advantages,
Hope those will be helpful for your choice.
3. Agilent Zorbax Eclipse Plus C18 HPLC Column
Introduction:
The Agilent Zorbax Eclipse Plus C18 HPLC Column is engineered for high-resolution and sensitive analytical applications. This column is particularly adept at handling both acidic and basic compounds, making it an excellent choice for complex sample matrices.
Features:
- Tailored for high resolution and sensitivity in chromatographic separations
- Exceptional peak shapes for precise quantification and identification
- Optimally designed to handle both acidic and basic compounds
- Suitable for a wide range of analytical applications, including pharmaceuticals and bioanalysis
Advantages:
- High Resolution: The Zorbax Eclipse Plus C18 column provides sharp and well-resolved peaks, enhancing the clarity and accuracy of separation, especially for complex mixtures.
- Sensitivity: Offers heightened sensitivity, which is essential for detecting low-abundance analytes and ensuring reliable quantitation.
- Versatility with Acidic and Basic Compounds: Effectively separates both acidic and basic compounds, providing flexibility in method development and robustness in routine analyses.
- Superior Peak Formation: Known for delivering superior peak shapes, this column ensures consistent and reproducible results, which is crucial for high-precision analytical work.
The Agilent Zorbax Eclipse Plus C18 HPLC Column is an ideal choice for analysts requiring high resolution and sensitivity, combined with the ability to handle a diverse range of compounds.
4. GL Sciences Inertsil ODS-3 HPLC Column
Introduction:
The GL Sciences Inertsil ODS-3 HPLC Column is highly regarded for its durability and the exceptional peak shapes it produces. This column is designed to operate effectively across a broad pH range, making it a versatile and reliable option for a variety of analytical applications.
Features:
- Renowned for durability and long-lasting performance
- Provides exceptional peak shapes, enhancing analytical accuracy
- Suitable for a wide range of pH conditions
- Ideal for various applications, including pharmaceuticals, environmental testing, and food analysis
Advantages:
- Durability: The Inertsil ODS-3 column is built to withstand rigorous use, offering long-term reliability and reducing the need for frequent replacements.
- Exceptional Peak Shapes: Delivers well-defined and symmetrical peaks, which are crucial for precise quantification and identification of analytes.
- Versatility Across pH Range: Capable of handling both acidic and basic conditions, providing flexibility in method development and robustness in routine analyses.
- Robust Performance: Known for consistent and reproducible results, making it a dependable choice for laboratories with diverse analytical needs.
The GL Sciences Inertsil ODS-3 HPLC Column is a robust and versatile solution, ideal for analysts requiring reliable performance across a wide range of applications and pH conditions.
5. YMC-Pack Pro C18 HPLC Column
Introduction:
The YMC-Pack Pro C18 HPLC Column is recognized for its superior peak shape and extended column life. This column is designed to perform well across a broad pH range, making it a versatile and durable option for various analytical applications.
Features:
- Noted for excellent peak shape, enhancing analytical precision
- Extended column life, ensuring long-term usability
- Capable of handling a wide pH range, from 1 to 10
- Suitable for diverse applications, including pharmaceuticals, biotechnology, and environmental analysis
Advantages:
- Remarkable Peak Shape: Provides sharp, symmetrical peaks, which are essential for accurate quantification and detection of analytes.
- Longevity: The YMC-Pack Pro C18 column is designed for durability, offering a longer lifespan compared to many other columns, which helps reduce operational costs.
- Wide pH Range Adaptability: Its ability to operate across a wide pH spectrum makes it adaptable for various analytical methods, enhancing its versatility.
- Versatile Application: Suitable for a broad range of applications, this column is an excellent choice for laboratories requiring reliable and consistent performance under different conditions.
The YMC-Pack Pro C18 HPLC Column is a dependable and versatile choice, offering excellent peak shapes and extended durability, ideal for a wide range of analytical challenges.
6. Shimadzu Shim-pack GIST C18 HPLC Column
Introduction:
The Shimadzu Shim-pack GIST C18 HPLC Column is highly regarded for its exceptional reproducibility and durability. This column is designed to handle both simple and complex mixtures, making it an ideal choice for various analytical fields, including pharmaceuticals, environmental studies, and food safety analysis.
Features:
- Renowned for high reproducibility, ensuring consistent analytical results
- Durable construction for long-lasting performance
- Suitable for analyzing both simple and complex mixtures
- Ideal for a wide range of applications, including pharmaceutical, environmental, and food safety analysis
Advantages:
- High Reproducibility: The Shim-pack GIST C18 column consistently delivers reliable results, making it a trusted choice for routine and complex analyses.
- Durability: Built to withstand rigorous use, this column offers extended service life, reducing the frequency of replacements and associated costs.
- Versatility: Its capability to handle diverse types of mixtures makes it suitable for various analytical applications, from simple to complex sample matrices.
- Wide Application Range: Ideal for use in critical fields such as pharmaceuticals, environmental monitoring, and food safety, where accuracy and reliability are paramount.
The Shimadzu Shim-pack GIST C18 HPLC Column is a versatile and robust solution for analysts seeking high reproducibility and durability across a range of challenging applications.
7. Sigma-Aldrich Ascentis® Express C18 HPLC Column
Introduction:
The Sigma-Aldrich Ascentis® Express C18 HPLC Column utilizes Fused-Core® particle technology, which enhances its performance in high-speed and high-efficiency liquid chromatography applications. This technology is particularly beneficial for achieving excellent resolution and sensitivity, making it an ideal choice for fast LC analyses.
Features:
- Incorporates Fused-Core® particle technology for superior performance
- High speed and efficiency, suitable for rapid LC applications
- Excellent resolution, crucial for separating complex mixtures
- High sensitivity, ideal for detecting low-abundance analytes
Advantages:
- Fast LC Applications: The Ascentis® Express C18 column is optimized for high-speed separations, significantly reducing analysis time while maintaining high-quality results.
- High Efficiency: Offers sharp and narrow peaks, which enhance the resolution and accuracy of separations, crucial for complex analytical challenges.
- Excellent Sensitivity: Capable of detecting low concentrations of analytes, making it valuable for quantitative and trace analysis.
- Advanced Particle Technology: The Fused-Core® technology improves column efficiency and durability, providing consistent and reliable performance over extended periods.
The Sigma-Aldrich Ascentis® Express C18 HPLC Column is a cutting-edge solution for chromatographers seeking fast, efficient, and sensitive separations in a variety of analytical settings.
8. uHPLCs New Design C18 HPLC Column
Introduction:
The uHPLCs New Design C18 HPLC Column is an innovative solution tailored for chromatographers who require exceptional aromatic selectivity and stability under high pH conditions. This column is equipped with high smooth column technology, enhancing its performance in complex separations.
Features:
- Provides excellent aromatic selectivity, ideal for separating aromatic compounds
- Stability under high pH conditions, broadening its application range
- High smooth column technology for enhanced separation efficiency
- Suitable for a wide array of analytical applications, including pharmaceuticals and chemical research
Advantages:
- Aromatic Selectivity: The column’s superior selectivity for aromatic compounds ensures precise separation, making it particularly useful for analyzing complex mixtures containing aromatic substances.
- Stability at High pH: Capable of maintaining performance in high pH environments, this column offers greater flexibility and robustness in method development.
- High Smooth Column Technology: Enhances column efficiency, providing sharper peaks and better resolution, which is crucial for accurate identification and quantification of analytes.
- Versatile Application: Ideal for a variety of applications, from pharmaceuticals to chemical analysis, offering reliable and reproducible results across different analytical challenges.
The uHPLCs New Design C18 HPLC Column is a top choice for those needing advanced separation capabilities, particularly for aromatic compounds and high pH conditions, backed by the latest column technology innovations.
9. Grace Vydac 201TP C18 HPLC Column
Introduction:
The Grace Vydac 201TP C18 HPLC Column is a specialized column renowned for its effectiveness in peptide and protein separations. This column is a preferred choice in proteomics and biotechnology due to its high resolution and unique selectivity for biomolecules.
Features:
- Specialized for peptide and protein separations
- Offers high resolution, crucial for detailed biomolecule analysis
- Unique selectivity tailored for biomolecules, enhancing separation quality
- Ideal for proteomics, biotechnology, and related fields
Advantages:
- High Resolution: The 201TP C18 column delivers sharp and well-resolved peaks, which are essential for the precise analysis of complex peptide and protein mixtures.
- Unique Selectivity for Biomolecules: This column’s design provides unique selectivity, making it particularly effective for separating and analyzing peptides, proteins, and other biomolecules.
- Specialized Application: Optimized for use in fields like proteomics and biotechnology, where accurate and reliable biomolecule analysis is critical.
- Consistent Performance: Known for its reliability and consistency, the Vydac 201TP C18 column ensures reproducible results across different batches and applications.
The Grace Vydac 201TP C18 HPLC Column is an excellent choice for researchers and analysts focusing on peptide and protein separations, offering unparalleled resolution and selectivity for biomolecular analyses.
10. Jasco X-LC® C18 HPLC Column
Introduction:
The Jasco X-LC® C18 HPLC Column is engineered for extra-low dispersion, making it an optimal choice for high-throughput and high-resolution systems. This column is particularly well-suited for sensitive applications that require lower detection limits, providing precise and accurate results.
Features:
- Designed for extra-low dispersion, enhancing sensitivity and resolution
- Suitable for high-throughput systems, increasing analytical efficiency
- High-resolution capabilities for detailed separations
- Ideal for sensitive applications, particularly those requiring lower detection limits
Advantages:
- Low Dispersion: The X-LC® C18 column minimizes peak broadening, which is crucial for maintaining the integrity of high-resolution separations and achieving precise quantitation.
- High Throughput: Designed to work efficiently in high-throughput systems, this column supports rapid analysis without compromising on quality, making it ideal for busy laboratories.
- Sensitivity: Offers enhanced sensitivity, which is vital for detecting and quantifying low-abundance compounds, making it a valuable tool in applications such as pharmaceuticals and environmental analysis.
- Versatile Performance: Suitable for a wide range of analytical applications, the Jasco X-LC® C18 column is a versatile choice for laboratories looking to optimize both resolution and throughput.
The Jasco X-LC® C18 HPLC Column is a top-tier choice for analysts requiring extra-low dispersion and high sensitivity, making it an excellent option for high-throughput and high-resolution systems.
Those just some Top10 C18 HPLC Column, so if you want to know more details of C18 HPLC Column and price, please feel free to contact uHPLCs by emal sales@uhplcs.com.
