Day: March 31, 2023

  Why do HPLC mobile phase solvents need to be filtered and deaerated To answer this question, let’s first understand what a mobile phase is. Simply put, a mobile phase is a fluid in which a carrier carries a substance into and through the stationary phase. The mobile phase can be either a water-organic mixture of the…

Hydrophilic interaction (Hilic) chromatography appears to be the same as normal phase chromatography in the stationary phase. Can the same column be used for normal phase chromatography and Hilic chromatography? Regarding the mobile phase, which is close to reversed-phase chromatography, what is the difference between the retention behavior of the two modes and the pattern…