How Sample Solvent Affects Peak Shape in HPLC (and How to Fix It)
1. Why Peak Shape Problems in HPLC Often Start with the Sample Solvent When troubleshooting poor peak shape in HPLC, many analysts focus first on the column, mobile phase, or detector settings. However, one of the most common and often overlooked causes is the sample solvent itself. In many cases, the sample is dissolved in…