FAQ
1. How to make the HPLC columns more durable?
– In order to maintain the initial performance of the chromatographic column stably for as long as possible, during use, it should be used according to the precautions recorded in the instruction manual of each chromatographic column, but it is also necessary to perform daily maintenance on the column. Such as optimizing the analysis conditions (eluent, feed flow rate, injected sample, etc.), cleaning the chromatographic column regularly, etc.
2. How to avoid damage to the aneroid chromatographic column and prolong its service life?
– In order to reduce the damage of liquid chromatographic columns, uHPLCs recommends that you take some precautions when using chromatographic columns. For example:
(1). Add an in-line filter to the liquid phase system;
(2). Use a guard column at the front of the column;
(3). Purify the sample.
3. How to better store liquid chromatography columns?
– In actual work, the storage of the liquid chromatography column will also affect the service life of the column. Then for the storage of chromatographic columns, there are also the following suggestions:
(1). For short-term storage
It should be stored under conditions close to those of the commonly used mobile phase. Also, be sure to remove buffer salts, acids, etc. from the system.
(2). For long-term storage
Store the chromatographic column in the corresponding solvent according to the instructions, remove the chromatographic column from the system, and block both ends with plugs.