c18 hplc column 4.6 and 250mm
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uHPLCs C18 HPLC Column 0.1µ, 0.4µ 250 Mm × 4.6 Mm
The C18 column is a durable reversed-phase HPLC column that offers longer life at extreme pH conditions and provides excellent reproducibility stability and efficient separation results.
Features and Applications:
- Strict quality control of alkaline and heave metals content during silica gel production process
- Narrow particle size and pore size distribution
- Unique production process ensuring high lot-to-lot reproducibility
- Good stability at higher temperatures
UHPLCS C18 HPLC column, 0.1µ, 0.4µ 250 mm × 4.6 mm
Method | HPLC, analytical |
Brand | uHPLCs |
Phase | USHA C18 |
USP listing | L1 |
Mode | Reversed phase (RP) |
Type | USHA HPLC column (analytical) |
Hardware | Stainless steel |
Column length | 250 mm |
Column inner diameter | 4.6 mm |
Particle shape | Spherical |
Particle size | 5 µm |
Recommended application(s) | Aldehydes, Basic, neutral or acidic drugs, Derivatized amino acids, Fat-soluble vitamins, Ketones, Pesticides, Phenolic compounds, Reversed phase, RP applications HPLC |
Endcapped | Yes |
Carbon content | 17.0 % |
pH stability | 2.0–8.0 |
Pore size | 100 Å |
Temperature stability (max) | 60 °C |
Tmax (phosphate buffer) | 40 °C |
Pressure stability (max) | 400 bar |
Scope of delivery | 1x analytical HPLC column, instruction leaflet, certificate of analysis |
Service
uHPLCs special services:
1. All columns will be tested before delivery and a column efficiency report will be provided.
2. Column loading service is available.
Features of C18 HPLC Column From uHPLCs
C18 HPLC columns are the most widely used type of column in HPLC. They are made of silica gel that has been modified with a layer of octadecylsilane (C18). This layer makes the column hydrophobic, meaning that it will preferentially retain nonpolar compounds. C18 columns are used to separate a wide variety of compounds, including organic molecules, pharmaceuticals, and environmental pollutants.
Some of the features of C18 HPLC columns include:
- High efficiency: C18 columns are very efficient, meaning that they can separate compounds with very close retention times. This makes them ideal for applications where high resolution is required, such as the analysis of complex mixtures.
- Wide range of applications: C18 columns can be used to separate a wide variety of compounds, making them a versatile tool for a variety of applications.
- Robustness: C18 columns are relatively robust and can withstand high pressures, making them a good choice for high-throughput applications.
C18 HPLC columns are a valuable tool for a variety of applications. They are highly efficient, versatile, and robust, making them a good choice for a wide range of analytical tasks.
Here are some additional details about the features of C18 HPLC columns:
- High efficiency: C18 columns are made with small particles, which increases the surface area of the column. This allows for more efficient separation of compounds.
- Wide range of applications: C18 columns can be used to separate a wide variety of compounds, including organic molecules, pharmaceuticals, and environmental pollutants. This makes them a versatile tool for a variety of applications.
- Robustness: C18 columns are relatively robust and can withstand high pressures. This makes them a good choice for high-throughput applications.
C18 HPLC columns are a valuable tool for a variety of applications. They are highly efficient, versatile, and robust, making them a good choice for a wide range of analytical tasks.
Application
C18 HPLC columns are used in a wide variety of applications, including:
- Pharmaceutical analysis: C18 columns are used to analyze the purity of pharmaceuticals, to identify impurities, and to determine the concentration of active ingredients.
- Environmental analysis: C18 columns are used to analyze environmental pollutants, such as pesticides, herbicides, and polycyclic aromatic hydrocarbons (PAHs).
- Food analysis: C18 columns are used to analyze food products for the presence of contaminants, such as pesticides, herbicides, and phthalates.
- Forensic analysis: C18 columns are used to analyze evidence in forensic investigations, such as drugs, explosives, and gunshot residue.
- Biotechnology: C18 columns are used to analyze biological samples, such as proteins, DNA, and RNA.
- Natural products: C18 columns are used to analyze natural products, such as essential oils, plant extracts, and marine compounds.
C18 HPLC columns are a versatile tool that can be used for a wide variety of applications. They are highly efficient, versatile, and robust, making them a good choice for a wide range of analytical tasks.
Here are some additional details about the applications of C18 HPLC columns:
- Pharmaceutical analysis: C18 columns are used to analyze the purity of pharmaceuticals by separating the active ingredient from other components in the drug. This is important to ensure that the drug is effective and safe to use.
- Environmental analysis: C18 columns are used to analyze environmental pollutants by separating them from other components in the environment. This is important to identify and track pollutants so that they can be removed or controlled.
- Food analysis: C18 columns are used to analyze food products for the presence of contaminants by separating the contaminants from other components in the food. This is important to ensure that food is safe to eat.
- Forensic analysis: C18 columns are used to analyze evidence in forensic investigations by separating the evidence from other materials at the crime scene. This is important to identify and collect evidence so that it can be used to solve crimes.
- Biotechnology: C18 columns are used to analyze biological samples by separating the components of the sample. This is important to study the structure and function of biological molecules.
- Natural products: C18 columns are used to analyze natural products by separating the components of the product. This is important to study the composition and properties of natural products.
C18 HPLC columns are a valuable tool for a variety of applications. They are highly efficient, versatile, and robust, making them a good choice for a wide range of analytical tasks.
FAQ of C18 HPLC Column
A 0.1µ C18 HPLC column is a high-performance liquid chromatography column that uses a C18 stationary phase with a particle size of 0.1µm for high-resolution and high-efficiency separation of complex sample mixtures.
The benefits of using this column include high-resolution separation, high efficiency and sensitivity, extreme stability, and versatility. This column allows for the separation of complex mixtures with faster and more accurate separations than previously possible.
This column is ideal for applications such as peptide mapping, proteomics, and small molecule analysis, as well as other analytical and preparative chromatography applications.
Most of the HPLC detectors, such as UV-Vis, PDA, fluorescence, and mass spectrometry, can be used with a 0.1µ C18 HPLC column.
The lifespan of a 0.1µ C18 HPLC column can vary depending on the sample type and other factors. Using proper care and maintenance, this column can provide consistent and reliable performance for many samples.
No, once the column has been used, it can’t be reused, as the stationary phase and particle size may become altered or contaminated.
The column should be flushed with suitable solvent after every use and stored in a cool, dry, and clean place. Ensure that the column is not exposed to excessive heat and humidity to avoid any damage.
Selecting the right column for your application requires careful consideration of your specific requirements, including compound properties, analysis conditions, and the column specification. It’s essential to consult with a trusted supplier or manufacturer to ensure the column is appropriate for your needs.
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