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1. Introduction to HPLC Column Size High-Performance Liquid Chromatography (HPLC) is a cornerstone of modern analysis across pharmaceuticals, biotechnology, food safety, and environmental testing. At the heart of every separation is the HPLC column, and its size—length, internal diameter (ID), particle size, and pore size—directly… Read More »HPLC Column Size – A Complete Guide to Dimensions, Particle Size, and Applications

Introduction High-Performance Liquid Chromatography (HPLC) is an essential analytical technique widely used in pharmaceuticals, food safety, environmental monitoring, and other industries. Despite its robustness, even experienced operators may encounter system malfunctions or decreased column efficiency. This article outlines the 10 most common HPLC problems and… Read More »Top 10 Common HPLC Problems and How to Fix Them

Introduction High Performance Liquid Chromatography (HPLC) is an essential analytical technique widely used in pharmaceutical, environmental, food, and chemical industries for the separation, identification, and quantification of complex mixtures. Its accuracy, sensitivity, and repeatability make it a cornerstone in modern analytical laboratories. At the heart… Read More »HPLC Column Leakage: A Practical Guide to Causes, Detection, and Troubleshooting

1. Introduction High-Performance Liquid Chromatography (HPLC) is a powerful and widely used analytical technique in chemistry, pharmaceuticals, environmental testing, food safety, and many other fields. It allows scientists to separate, identify, and quantify components in complex mixtures with high precision and accuracy. As a fundamental… Read More »Retention Time Drift in HPLC: Causes, Corrections, and Prevention

In the world of analytical chemistry, chromatography plays a vital role in separating, identifying, and quantifying components in complex mixtures. Among the most widely used chromatographic techniques are Liquid Chromatography (LC) and Gas Chromatography (GC). While both aim to achieve separation and analysis, they operate… Read More »LC vs GC: Key Differences, Applications, and How to Choose

High-performance liquid chromatography (HPLC) plays a critical role in analytical laboratories for the separation, identification, and quantification of compounds. One of the key indicators of HPLC performance is column efficiency. High column efficiency not only improves resolution and peak shape but also leads to faster… Read More »How to Improve Your HPLC Column Efficiency

High-performance liquid chromatography (HPLC) systems rely on a range of components, from tubing and fittings to valves and columns, to ensure accurate, reliable separations. Among the critical decisions in system setup and maintenance is the choice of materials for these components. Two of the most… Read More »Choosing Between Stainless Steel and PEEK Components in HPLC

In high-performance liquid chromatography (HPLC), accuracy and consistency are paramount. However, even with the most precise instrumentation and high-quality reagents, your analysis can be compromised by one often-overlooked factor: contamination from particulates. These microscopic invaders—originating from solvents, samples, or worn system components—can clog columns, damage… Read More »Three Essential HPLC Filters for Purity in Your Analyses

Types of Columns Used in HPLC Introduction High-performance liquid chromatography (HPLC) is a technique that is used to separate and quantify the components of a mixture. It does this by using a column that is packed with a stationary phase. The stationary phase can be… Read More »How Many Types of Columns Used in HPLC ?